The composition of bile acids in patients with cholelithiasis according to the data of liquid chromatography with mass spectrometric detection

Authors

  • V. M. Klymenko Zaporizhzhia State Medical University, Ukraine,
  • D. V. Syvolap Zaporizhzhia State Medical University, Ukraine,
  • A. G. Kaplaushenko Zaporizhzhia State Medical University, Ukraine,
  • B. O. Varynskyi Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2017.6.114710

Keywords:

bile acids, cholelithiasis, liquid chromatography, mass spectrometric detection

Abstract

Bile acids play a leading role in the physical and colloidal properties of bile stabilization. Lack of bile acids consequences result in the formation of cholesterol stones in the gall bladder, diarrhea and steatorrhea, fat-soluble vitamins impaired absorption, and kidney stones formation (oxalates).

Investigation of altered bile composition, especially the content of bile acids, in patients with gallstone disease by means of modern analytical analysis methods (liquid chromatography with mass spectrometric detection) would complement the modern ideas about mechanisms of lithogenesis and aim efforts at prevention of stone formation in the gall bladder, that was the purpose of our work.

Materials and methods. Bile samples were tested for bile acid content using liquid chromatography with mass spectrometry. 14 samples of bile from patients with cholelithiasis were included in the main group, and control group consisted of 7 bile samples from practically healthy persons.

Results. In patients with cholelithiasis there is an increase in the content of conjugated forms of bile acids – glycolic acid in 2 times (p = 0.002), taurocholic acid in 1.57 times (p = 0.062) compared with practically healthy persons. In patients with cholelithiasis, the ratio of taurocholic to glycolic acidі content (0.95 vs. 1.27, p = 0.0179), as well as glycogenodeoxycholic to glycodeoxycholic acid (1.11 vs. 1.58, p = 0.027) is significantly less than that in practically healthy persons. In addition, one in two patients with cholelithiasis does not reveal the presence of ursodeoxycholic acid in the bile.

Conclusions. The lithogenic properties of bile are primarily caused by conjugated forms of cholic acid with glycine and taurine content violation. The ratio of taurocholic to glycolic acid content in patients with cholelithiasis is significantly lower than the similar index in practically healthy persons (0.95 vs. 1.27, p = 0.0179). The ratio of glycine conjugated bile acids content, namely glycogenodeoxycholic to glycodeoxycholic acid, in patients with cholelithiasis is also significantly lower than in practically healthy individuals (1.11 versus 1.58, p = 0.027). Ursodeoxycholic acid is not defined in bile of one in two patients with cholelithiasis (50 %), while it is absent in every seventh among practically healthy persons (14.29 %).

References

Ivaschkin, V. Т. (Ed.) (2008) Bolezni pecheni i zhelchevyvodyaschikh putej [Diseases of the liver and biliary tract]. Мoscow: М.-Vesty. [in Russian].

Kozlov, А. V., Slepysheva, V. V., & Rebyakova, E. N. (2013) Metody opredeleniya zhelchnyh kislot [Metods of estimation bile acids]. Terra Medica. Laboratornaya diagnostica, 1(28), 3–8. [in Russian].

urkard, I., von Eckardstein, A., & Rentsch, K. M. (2005). Differentiated quantification of human bile acids in serum by high-performance liquid chromatography–tandem mass spectrometry. J. Chromatogr, 826(1-2), 147–59. doi: 10.1016/j.jchromb.2005.08.016.

Carey, M. C., Duane, W. C., Arias, I. M., Boyer, J. L., Fausto, N., Jakoby, W. B., et al. (Eds.) (1994). The Liver: Biology and Pathobiology. New York: Raven Press.

Hofmann, A. F., Arias, I. M., Boyer, J. L., Fausto, N., Jakoby, W. B., Schachter, D. A., & Shafritz, D. A. (Eds.) (1994). The Liver: Biology and Pathobiology. New York: Raven Press.

Jansen, P. L., & Groen, A. K. (2006) Mechanisms of bile secretion. Zakim and Boyer's Hepatology. A Textbook of liver Disease. M.D. Boyer, T.L. Wright, M.P. Manns (Eds). (Vol. 1), (P. 67–85).

Kuntz, E., & Kuntz, H.-D. (2006) Hepatology principles and practice. Springer-Verlag Berlin, Heidelberg.

Palmeira, C. M., & Rolo, A. P. (2004) Mitochondrially-mediated toxicity of the bile acids. Toxicology, 203(1-3), 1–15. doi: 10.1016/j.tox.2004.06.001.

Roda, A., Minutello, A., Angellotti, M. A., & Fini, A. (1990) Bile acid structure-activity relationship: evaluation of bile acid lipophilicity using I-octanol/water partition coefficient and reverse phase HPLC. Journal of Lipid Research, 31, 1433–1443.

Downloads

How to Cite

1.
Klymenko VM, Syvolap DV, Kaplaushenko AG, Varynskyi BO. The composition of bile acids in patients with cholelithiasis according to the data of liquid chromatography with mass spectrometric detection. Zaporozhye Medical Journal [Internet]. 2017Nov.15 [cited 2024Nov.25];(6). Available from: http://zmj.zsmu.edu.ua/article/view/114710

Issue

No. 6 (2017)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)