Dynamics of α-D-mannose carbohydrate-binding sites distribution in rats’ pancreatic structures during the morpho-functional formation
DOI:
https://doi.org/10.14739/2310-1210.2017.6.114718Keywords:
pancreas, rats, antigen, carbohydrates, mannoseAbstract
Recently, the pathology of pancreas in children is observed more frequently. Precursors of inflammation development in pancreas are dysfunctional disorders, which include conditions accompanied by changes in pancreatic secretion in the absence of morphological abnormalities. The pancreas is especially sensitive to irritants in the antenatal period of development and during its morpho-functional formation. Placental function insufficiency and prenatal effects of various pathogens on the fetus can cause abnormalities of morphogenesis. The above determines the relevance of the study, which allows ascertaining the features of the fetus pancreas formation in conditions of antigenic nature substances action.
The aim – to study the dynamics of α-D-Mannose carbohydrate-binding sites distribution in rats’ pancreatic structures during the morpho-functional formation, using lectin histochemistry analysis.
Material and мethods. Research was conducted on pancreases of white laboratory rats from the day of birth to ninety days of postnatal development. The experimental group of rats was injected intrafetal with an antigenic solution subcutaneously in the interscapular region. Detection of carbohydrate residues of α-D-Mannose was carried out by lens culinaris agglutinin (LCA), which conjugated with horseradish peroxidase.
Results. Antigenic action in the antenatal period results to decrease in density of α-D-Mannose residues in pancreatic structures from the first to the third day of postnatal life. From the seventh day and until the fourteenth day the density of LCA receptors in experimental group has increased. From the twenty first until ninetieth days of life the distribution of carbohydrate receptors to LCA in the experimental group was not differ from the control and intact group.
Conclusions. The increase in density of carbohydrate residues of α-D-Mannose in pancreatic structures in early postnatal period is the sign of immaturity, which may have the direct effect on secretory activity of organ.
References
Antonyuk, R. V., & Lutsyk, O. D. (2015). Lektynova histokhimiia vuhlevodnykh determinant kyshky predstavnykiv riznykh klasiv khrebetnykh [Lectin histochemistry of intestinal carbohydrate determinants in representatives of different classes of vertebrates]. Morfolohiia, 9(4), 7–20. [in Ukrainian].
Boiko, V. V., Akhtemiichuk, Yu. T., & Slobodian O.M. (2008). Suchasni pohliady na poshkodzhennia pidshlunkovoi zalozy (khirurhichni aspekty) [Modern views on damage of the pancreas]. Ukrainskyi zhurnal khirurhii, 1, 87–89. [in Ukrainian].
Chu, L., Zhang, J., Li, Y. N., Meng, X., & Liu, Y. Y. (2016). Clinical treatment of infective endocarditis with vegetations in pregnant women and the outcomes of gestation. Zhonghua Fu Chan Ke Za Zhi., 51(5), 331–8. doi: 10.3760/cma.j.issn.0529-567X.2016.05.003.
Dovgal,´ G. V., Dovgal,´ M. A., Supon´ko, Yu. V., & Voloshin, N. A. (2016). Gistokhimicheskaya dinamika glikozaminoglikanov v podzheludochnoj zheleze krys pri estestvennom izmenenii tipa pitaniya v postnatal'nom periode [Histochemical dynamics of glycosaminoglycans in the pancreas of rats with the natural change of the type of power in the postnatal period]. Visnyk problem biolohi ta medytsyny, 2(129), 27–29. [in Ukrainian].
Koctiuk, H. Ya. (2014). Naslidky pidvyshchenoho tysku u prototsi pidshlunkovoi zalozy [Consequences of increased pressure in the pancreatic duct]. Visnyk Vinnytskoho natsionalnoho medychnoho universytetu, 1(18), 30–32. [in Ukrainian].
Maslova, I. N. (2014). Glyconjugates distribution features in the rat’s major salivary glands structures after antenatal antigen action. Ukrainian scientific medical youth jornal. 4(83), 135–136.
Oz, H. S., & Tobin, T. (2012). Atovaquone ameliorate gastrointestinal toxoplasmosis complications in a pregnancy model. Med Sci. Monit., 18(9), BR337–345. doi: 10.12659/MSM.883342.
Slobodian, O. M. (2013). Histotopohrafichni osoblyvosti pankreatoduodenalnoho orhanokompleksu u plodiv ta novonarodzhenykh [Histotopographic features of the pancreatoduodenal organo – complex in fetus and newborns]. Morfolohiia, 2(4), 47–50. [in Ukrainian].
Voloshin, N. A., & Grigor´eva, E. A. (2005). Lektiny zhivotnogo i rastitel'nogo proiskhozhdeniya: rol' v processakh morfoheneza [Lectins of animal and vegetable origin: a role in morphogenesis processes]. Zhurnal Akademii medicinkikh nauk Ukrainy, 11(2), 223–227. [in Russian].
Voloshin, N. A., Grigor´eva, E. A., & Dovbysh, M. A. (2004). Ispol'zovanie metodov lektinovoj gistologii v citologii [The use of the lectin in histology and cytology]. Tavricheskij mediko-biologicheskij vestnik, 7(4), 40–41. [in Russian].
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