Antimicrobial effect of proteflazid extract on microflora of peri-implant areas in infectious and inflammatory complications after dental implantation

Authors

  • M. M. Ananieva Higher State Educational Institution of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • M. O. Faustova Higher State Educational Institution of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • Ya. O. Basarab Higher State Educational Institution of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • G. A. Loban’ Higher State Educational Institution of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,

DOI:

https://doi.org/10.14739/2310-1210.2017.6.115264

Keywords:

proteflazid extract, flavonoids, peri-implantitis, microbial sensitivity tests, antimicrobial agents

Abstract

The purpose of this study was to investigate the proteflazid extraction antimicrobial action onto causative agents of infectious and inflammatory diseases occurring during dental implant placement.

Materials and Methods. We studied clinical strains of Streptococcus sanguinis, Staphylococcus warneri and Kocuria kristinae, isolated from oral mucosa of patients who were diagnosed with peri-implant mucositis and peri-implantitis. The cultivation of strains was carried out by standard method. Final identification was carried out with an automatic bacteriological analyzer Viteck 2 compact bioMérieux (France) according to manufacturer's instructions. The sensitivity of derived strains to proteflazid and to 96 % ethyl alcohol assessment was done by double serial dilutions according to the standard procedure.

Results. The clinical strains of Streptococcus sanguinis, Staphylococcus warneri and Kocuria kristinae were found to be sensitive to effects of proteflazid extract. K. kristinae was proven to be the least susceptible strain. But the clinical strain of Streptococcus sanguinis was found to be the most sensitive to proteflazid extract. 96 % ethyl alcohol, which is a component of the extract, showed a bactericidal effect on the clinical strains in the study, but its minimal bactericidal concentration was significantly lower than minimal bactericidal concentration of the proteflazid extract.

Conclusions. The proteflazid extract can produce antimicrobial effect on the clinical strains of Streptococcus sanguinis, Staphylococcus warneri and Kocuria kristinae, which colonize the oral mucosa of patients with infectious and inflammatory complications following dental implant placement. Antimicrobial action of the proteflazid extract is much higher than action of 96% ethyl alcohol that confirms the direct antimicrobial action of flavonoids, which are components of the studied extract.

References

Kawamata, H., Hakata, K., Okubo, M., & Uchida, D. (2015). Association of the oral bacteria with onset of the infective endocarditis. International Journal of Oral and Maxillofacial Surgery, 44, e240.

DeSimone, D. C., DeSimone, C. V., Tleyjeh, I. M., de Sa, D. D. C., Anavekar, N. S., Lahr, B. D. & Baddour, L. M. (2015). Association of mitral valve prolapse with infective endocarditis due to Viridans Group Streptococci. Clinical Infectious Diseases, 61(4), 623–625. doi: 10.1093/cid/civ375.

Duggal, S., & Chugh, T. D. (2014). Pasteurella multocida: Cause of subdural and paranasal sinus abscesses in an adolescent. Journal of Pediatric Infectious Diseases, 9(2), 115–118. doi: 10.3233/JPI-140423.

Bhardwaj, B., Bhatnagar, U. B., & Conaway, D. G. (2016). An Unusual Presentation of Native Valve Endocarditis Caused by Staphylococcus warneri. Reviews in Cardiovascular Medicine, 17.

Petrushanko, T. A., Tchereda, V. V., & Loban, G. A. (2014). Skriningovaya diagnostika mikroe´kologicheskikh narushenij polosti rta [The screening diagnostic of micro ecological disorders of oral cavity]. Klinicheskaya laboratornaya diagnostika, 59(6), 48–50. [in Russian].

Ahmed, N. H., Biswal, I., Roy, P., & Grover, R. K. (2014). Kocuria kristinae, an unusual pathogen causing opportunistic infections in patients with malignancy. Indian journal of medical microbiology, 32(40), 456. doi: 10.4103/0255-0857.142232.

Citro, R., Prota, C., Greco, L., Mirra, M., Masullo, A., Silverio, A., et al. (2013). Kocuria kristinae endocarditis related to diabetic foot infection. Journal of medical microbiology, 62(6), 932–934. doi: 10.1099/jmm.0.054536-0.

Faustova, M. O., Ananieva, M. M., & Basarab, Ya. O. (2016). Bakterytsydna ta funhitsydna aktyvnist ekstraktu proteflazydu shchodo muzeinykh shtamiv mikroorhanizmiv Staphylococcus aureus AT•SS 25923, Staphylococcus epidermidis AT•SS 14990, Escherichia coli AT•SS 25922, Streptococcus faecalis AT•SS 29212, Micrococcus luteus ATCC 4698, Candida albicans AT•SS10231. [Bactericidal and fungicidal activity of the extract on proteflazid museum strains Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 14990, Escherichia coli ATCC 25922, Streptococcus faecalis ATCC 29212, Micrococcus luteus ATCC 4698, Candida albicans ATSS10231]. Aktualni problemy suchasnoi medytsyny, 16, 4(56), 72–74. [in Ukrainian].

Kaminskyi, V. V., Shalko, M. N., Michailov, V. S., Lesnikova, M. V., Yashchenko, L. A., Grynevych, O. I., & Broun, T. A. (2015). Ocenka e´ffektivnosti preparata Proteflazyd pry lechenii papillomavirusnoj infekcii: meta-analiz rezulʹtatov mnogoletnikh klinicheskikh issledovanij [Evaluation of efficacy of Proteflazid in the treatment of HPV-infection: meta-analysis of long-term clinical trials results]. Medicinskie aspekty zdorov'ya zhenshchiny, 6(92), 5–14. [in Russian].

Nakaz Ministerstva okhorony zdorovia Ukrayiny «Pro zatverdzhennia metodychnykh vkazivok «Vyznachennia chutlyvosti mikroorhanizmiv do antybakterialʹnykh preparativ» vid 05.04.2007 r. №167 [Order №167 of the Ministry of Public Health of Ukraine on "On Approval of Training Guidance "Assessment of the sensitivity of microorganisms to antibiotics ", April, 5, 2007]. Retrieved from: http//www.moz.gov.ua. [in Ukrainian].

Downloads

How to Cite

1.
Ananieva MM, Faustova MO, Basarab YO, Loban’ GA. Antimicrobial effect of proteflazid extract on microflora of peri-implant areas in infectious and inflammatory complications after dental implantation. Zaporozhye Medical Journal [Internet]. 2017Nov.15 [cited 2024Dec.27];(6). Available from: http://zmj.zsmu.edu.ua/article/view/115264

Issue

No. 6 (2017)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)