Histological research of hepatoprotective activity of tablets Lavaflam in rats with subchronic hepatitis

Authors

  • M. A. Aslanian National University of Pharmacy, Kharkiv, Ukraine,
  • L. A. Bobrytska National University of Pharmacy, Kharkiv, Ukraine,
  • N. L. Bereznyakova National University of Pharmacy, Kharkiv, Ukraine,
  • O. S. Shpychak National University of Pharmacy, Kharkiv, Ukraine,
  • V. I. Hrytsenko National University of Pharmacy, Kharkiv, Ukraine,
  • T. A. Germanyuk National Pirogov Memorial Medical University, Vinnytsia, Ukraine,
  • T. I. Ivko National Pirogov Memorial Medical University, Vinnytsia, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.02.124940

Keywords:

liver diseases, bile duct diseases, histology, Lavaflam

Abstract

Hepatobiliary system diseases are medical and social problem at the present time. Important issue is the development of a new complex of herbal medicine and its pharmacoeconomic study.

The purpose of the work was an experimental substantiation of the hepatoprotective properties of the drug Lavaflam.

Methods of research: pharmacological, morphological, histological, methods of mathematical statistics.

Materials of research. Experimental pharmacological studies were performed on the white rats weighing 180–220 g. The study was carried out in the Central Research Laboratory (CRL) of the National University of Pharmacy,Kharkіv,Ukraine (Certificate № 023/13 of 05.03.2013) according to the methodological recommendations of theStateExpertCenter of the Ministry of Health ofUkraine. The rats were divided into four experimental groups: the first group – intact control; the second group – positive pathology – the animals were injected TChM; the third group – animals, received TChM and Lavaflam; the fourth group – animals, received TChM and reference drug Сarsil.

Results. It has been found that the test drug Lavaflam showed hepatoprotective properties in experimental model of the subchronic hepatitis (diffuse inflammatory liver disease) in rats.

Conclusions. Lavaflam, in comparison with Carsil contributed to the elimination and reduction in a number of pathological changes, increased adaptive capacity of hepatocytes, and Lavaflam promoted the physiological regeneration of cells. Relating to the effect of a positive influence on the hepatic parenchyma condition with induced subchronic hepatitis by TChM, the drug Lavaflam was not inferior to the comparison drug Carsil (by the effect on necrotic manifestations), or was superior to the comparison drug Carsil (by the zones of destruction spread, dystrophic changes in hepatocytes).

References

Mykhailiuk, Ye. O. (2015). Farmakolohichna aktyvnist novykh pokhidnykh 4-r-5-(alkil-, aryl-,heteryl-)-1,2,4-triazolu pry eksperymentalnomu toksychnomu hepatyti (Avtoref. dis…kand. tekh. nauk). [Pharmacological activity of new derivatives of 4-r-5- (alkyl-, aryl-, heteryl) -1,2,4-triazole in experimental toxic hepatitis]. (Extended abstract of candidate’s thesis). Zaporizhzhia [in Ukrainian].

(2017) WHOCC-ATC / DDD Index. Retrieved from https://www.whocc.no/atc_ddd_index.

Bobryts'ka, L.A., Arakelyan, M.A., Popova, N.V. (2014). Marketynhove doslidzhennya rynku likiv z zhovchohinnoyu diyeyu [Marketing research of the market of drugs with the choleretic action]. Visnyk farmatsiyi, 2, 63-67. [in Ukrainian].

Aslanyan, M. A. (patentee), Bobrytska, L., Honcharov, M. I., Nazarova, E. S., Popova, N. V., & Lytvynenko, V. I. (2015) Patent 97298 Ukrayina, MPK A 61 K 36/53. Likarskyi zasib zhovchohinnoho efektu [Patent 97298 Ukraine, IPC A 61 K 36/53. Medicinal product of choleretic effect]. Bulleten, 5. [in Ukrainian].

Bobrytska, L. A., Arakelian, M. A., & Popova, N. V. (2014) Marketynhove doslidzhennia rynku likiv z zhovchohinnoiu diieiu [Market research of the market of medicines with choleretic effect]. Visnyk farmatsii, 2(78), 63–67. [in Ukrainian].

Stefanov, O. V. (ed) (2001). Doklinichni doslidzhennia likiv [Preclinical research of medicines]. Kyiv: Avitsenna [in Ukrainian].

(1986). European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe, Strasbourg.

Pozdniakov, V. S., & Ivanov, N. H. (1979). Zmina funktsionalnoho stanu shchuriv pid vplyvom tetrakhlorydu vuhletsiu [Changing the functional state of rats under the influence of carbon tetrachloride]. Toksykolohiia novykh promyslovykh khimichnykh substantsii, 15, 87–90 [in Ukrainian].

Rybolovlev, Yu. R., & Rybolovlev, R. S. (1979). Dozirovanie veshhestv dlya mlekopitayushchikh po konstantam biologicheskoj aktivnosti [Dosage of substances mammals on biological activity constants]. Doklady AN SSSR, 247(6), 1513–1516 [in Russian].

Halafyan, A. A. (2007) STATISTICA 6. Statisticheskij analiz danykh [Statistics 6. Statistical analysis of data]. Moscow: Binom Press [in Russian].

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How to Cite

1.
Aslanian MA, Bobrytska LA, Bereznyakova NL, Shpychak OS, Hrytsenko VI, Germanyuk TA, Ivko TI. Histological research of hepatoprotective activity of tablets Lavaflam in rats with subchronic hepatitis. Zaporozhye Medical Journal [Internet]. 2018Mar.7 [cited 2024Nov.23];(2). Available from: http://zmj.zsmu.edu.ua/article/view/124940

Issue

No. 2 (2018)

Section

Original research

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