Efficiency of assisted hatching of the cryopreserved–melted embryos

Authors

  • V. A. Pitko Government institution «Ukrainian Medical Center of Obstetrics, Gynecology and Reproductology Ukrainian Ministry of Health», Kharkiv, Ukraine,
  • А. Yu. Shcherbakov Kharkiv Medical Akademy of Postgraduate Education, Kharkiv, Ukraine,
  • O. A. Lohinova Kharkiv Medical Akademy of Postgraduate Education, Kharkiv, Ukraine,
  • N. N. Sinilo Government institution «Ukrainian Medical Center of Obstetrics, Gynecology and Reproductology Ukrainian Ministry of Health», Kharkiv, Ukraine,
  • Е. Ye. Nipot Institute of Problems of Cryobiology and Cryomedicine NAS of Ukraine, Kharkiv, Ukraine,
  • Ya. O. Cherkashina Institute of Problems of Cryobiology and Cryomedicine NAS of Ukraine, Kharkiv, Ukraine,
  • I. V. Pavlov Government institution «Ukrainian Medical Center of Obstetrics, Gynecology and Reproductology Ukrainian Ministry of Health», Kharkiv, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.02.125274

Keywords:

embryos, vitrification, pellucida zona, embryo transfer, pregnancy

Abstract

Purpose. To measure outcomes of clinical research of efficiency of assisted hatching of cryopreserved embryos.

Materials and methods. Patients who had un successful cycles IVF/ICSI with transfer of fresh embryos have been selected for participation in the research between 2014 and 2016 years. Patients were distributed in a random way for participation in the experiment and control groups. Results of embryos transfer of one or two cryopreserved and melted embryos were considered only. Embryos were cryopreserved at a stage of blastocyst, 5 days after extraction of oocytes by method of vitrification. Melting procedure was conducted in the morning of a day of embryos transfer following the instructions of the vitrification medium producer Cryotech (Japan). Assisted hatching was conducted with use of micropipettes of Holding Pipette Cook Medical (Australia) and Assisted Hatching/Zona Drilling Pipette Cook Medical (Australia). The treated embryos were cultivated up to a repeated estimation of morphology of embryos before transfer. Transfer of embryos has been conducted by a standard method with the use of catheter for non-invasive transfer of embryo Sydney IVF Cook Medical (Australia). The quantity of the transferred embryos varied from one to two.

Results. 100 cryopreserved embryos were transferred which have been distributed in a random way either to the group with the assisted hatching or to the control group (without assisted hatching). A number of parameters of patients from both groups was analyzed, i.e. age of the patient at the time of melting of embryos, duration of infertility, causes of infertility, quantity of previous unsuccessful cycles IVF/ICSI. Any essential differences between patients within two groups based on the aforementioned parameters were not revealed. Also, there were no essential differences in number of the melted embryos, survival rate of embryos, quantity of the embryos transferred to patients. However, at the same time, parameters of positive results of tests on HCG (human chorionic gonadotropin) (42 % against 27 %) and quantity of clinical pregnancy (35 % against 24 %) were statistically higher in the group with assisted hatching comparatively to the control group (P < 0.004; P < 0.001 accordingly).

Conclusions. Implantation of the transfered embryos and number of clinical pregnancies were statistically improved due to selection of the optimum freezing conditions and subsequent cultivation and conducting of procedure of mechanical incision of ZP.

References

Hammadeh, M. E., Fischer-Hammadeh, C., & Ali, K. R. (2011) Assisted hatching in assisted reproduction: a state of the art. J AssistReprodGenet., 28(2), 119–128. doi: 10.1007/s10815-010-9495-3.

Kissin, D. M., Kawwass J. F., Monsour, M., Boulet, S. L., Session D. R., Jamieson D. J., et al. (2014) Assisted hatching: trends and pregnancy outcomes, United States, 2000–2010. Fertility and Sterility, 102(3), 795–801. doi: 10.1016/j.fertnstert.2014.06.013.

Li, Z., Wang, Y., Ledger, W., Edgar, D., & Sullivan, E. (2014). Clinical outcomes following cryopreservation of blastocysts by vitrification or slow freezing: a population-based cohort study. Human Reproduction, 29(12), 2794–2801. doi: 10.1093/humrep/deu246.

Ghobara, T., Gelbaya, T. A., & Ayeleke, R. O. (2017) Cycle regimens for frozen-thawed embryo transfer. Cochrane Database of Systematic Reviews, 7, 1–102. doi: 10.1002/14651858.CD003414.pub3.

Ge, H., Zhou, W., Zhang, W., & Lin, J. (2008). Impact of assisted hatching on fresh and frozen–thawed embryo transfer cycles: a prospective, randomized study. Reproductive Bio Medicine Online, 16(4), 589–596. doi: 10.1016/s1472-6483(10)60466-x.

Cohen, J., Elsner, C., Kort, H., Malter, H., Massey, J., Mayer, M., & Wiemer, K. (1990). Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Human Reproduction, 5(1), 7–13. doi: 5(1), 7–13.

Petersen, C. G., Mauri, A. L., Baruffi, R. L., Oliveira, J. B., Felipe, V., Massaro, F. C., & Franco, J. G. (2006) Laser-assisted hatching of cryopreserved-thawed embryos by thinning one quarter of the zona. Reproductive Bio Medicine Online, 13(5), 668–675. doi: 10.1016/s1472-6483(10)60657-8.

Fang, C., Li, T., Miao, B., Zhuang, G., & Zhou, C. (2010). Mechanically expanding the zona pellucida of human frozen thawed embryos: a new method of assisted hatching. Fertilityand Sterility, 94(4), 1302–1307. doi: 10.1016/j.fertnstert.2009.08.014.

Jwa, J., Jwa, S., Kuwahara, A., Yoshida, A., & Saito, H. (2015). Risk of major congenital anomalies after assisted hatching: analysis of three-year data from the national assisted reproduction registry in Japan. Fertility and Sterility, 104(1), 71–78. doi: 10.1016/j.fertnstert.2015.03.029.

Kanyo, K., Zeke, J., Kriston, R., Szücs, Z., Cseh, S., Somoskoi, B., & Konc, J. (2016). The impact of laser-assisted hatching on the outcome of frozen human embryo transfer cycles. Zygote, 24(5), 742–447. doi: 10.1017/S0967199416000058.

Downloads

How to Cite

1.
Pitko VA, Shcherbakov АY, Lohinova OA, Sinilo NN, Nipot ЕY, Cherkashina YO, Pavlov IV. Efficiency of assisted hatching of the cryopreserved–melted embryos. Zaporozhye Medical Journal [Internet]. 2018Mar.7 [cited 2024Nov.13];(2). Available from: http://zmj.zsmu.edu.ua/article/view/125274

Issue

No. 2 (2018)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)