Antibiotic-associated disorders of prothrombin synthesis and their probiotic correction with B. Clausii in breastfeed infants

Authors

  • O. G. Ivanko Zaporizhzhia State Medical University, Ukraine,
  • O. V. Solianyk Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.3.130489

Keywords:

infant, Baccilus clausi, , breast seeding, decarboxyprothrombin, blood coagulation tests, anti-bacterial agents

Abstract

The aim of the investigation was to study the prothrombin test and PIVKA-II serum levels changes in infants aged 1-6 month after the broad-spectrum antibiotic treatment and determine the possibility of coagulation disorders correction with the help of probiotic Bacillus clausii strains OC, NR, T, SIN spores capable to produce vitamin K2.

Materials and methods. The study involved 57 convalescents after an acute respiratory infection exclusively breastfed infants aged 1 to 6 months one week after the 7-day parenteral treatment with ceftriaxone. Among them 13 children with laboratory signs of blood hypocoagulation (INR >1.3) had received probiotic Bacillus clausii spores in a daily dose of 2 × 109 CFU for 10 days. Another 13 children who had not received a probiotic made up a comparison group. Laboratory data was compared with the results of 27 healthy children of the control group. Prothrombin test parameters as a prothrombin (PT), prothrombin time (PTT), international normalized ratio (INR) and protein induced by vitamin K absence or antagonist II (PIVKA-II) were determined using the hematologic analyzer COAGUCHEC XS and ELISA kit.

Results. It was found that after antibacterial treatment in infants PT was decreased and reached only 50 % of the norm. At the same time, PTT and INR were increased up to 2-folds together with a significant PIVKA-II elevation, which is typical for vitamin K deficiency. The use of probiotic treatment within 10 days has contributed to normalization of prothrombin in 11 out of 13 observed infants versus 3 out of 13 in the comparison group (χ2 = 7,58 corrected by Yates, P < 0.05).

Conclusions. Cephalosporin administration for breastfed infants can lead them to latent vitamin K deficiency development, which is manifested by high levels of PIVKA-II and signs of hypocoagulation development revealed by prothrombin test. The Bacillus clausii probiotic spores prescription to infants after antibiotic therapy course for 10 days normalizes the plasma prothrombin concentration while in children of the comparison group it remains lower than normal.

 

References

Nakaz Ministerstva okhorony zdorovia Ukrainy «Pro zatverdzhennia Protokolu medychnoho dohliadu za zdorovoiu novonarodzhenoiu dytynoiu» vid 04.04.2005 riku №152 [Order of the Ministry of Health of Ukraine «Protocol for the care of a healthy newborn» from April, 4, 2005, №152]. [in Ukrainian].

Ivan'ko O. G., & Solyanik, A. V. (2016). PIVKA-II kak pokazatel' deficyta vitamina K u detej pervykh mesyacev zhizni, poluchayushhikh isklyuchitel'no grudnoe vskarmlivanie. Ukrainskoe issledovanie [PIVKA-II as an indicator of vitamin K deficiency in children of the first months of life who are exclusively breastfed. The Ukrainian study]. Zdorov'e rebenka, 7(75), 95–100 [in Russian].

Novikova, N. A., & Volovchenko, A. N. (2016). Varfarin: mesto v sovremennoj antikoagulyantnoj terapii [Warfarin: place in the anticoagulant therapy today]. Aterotromboz, 1, 50–58 [in Russian].

Chawla, D., Deorari, A. K., Saxena, R., Paul, V. K., Agarwal, R., Biswas, A., & Meena, A. (2007) Vitamin K1 versus vitamin K3 for prevention of subclinical vitamin deficiency: a randomized controlled trial. Indian Pediatrics, 44(11), 817–22.

Ero, M. P., Harvey, N. R. Harbert, J. L., Janc, J. W., Chin, K. H., & Barriere, S. L. (2014) Impact of telavancin on prothrombin time and activated partial thromboplastin time as determined using point-of-care coagulometers. J Thromb Thrombolysis, 38(2), 235–40. doi: 10.1007/s11239-013-1004-y.

Hosoi, T. & Kiuchi, K. (2003) A food made by fermenting cooked soybeans with Bacillus subtilis. Handbook of fermented Functional Foods. Boca Raton, Fla: CRC Press,.

Yong, A. B. (patentee) (2011) Patent WO20111589989 (A1) Strain of bacillus amyloliquefaciens having a high vitamin k2 producing ability.

Downloads

How to Cite

1.
Ivanko OG, Solianyk OV. Antibiotic-associated disorders of prothrombin synthesis and their probiotic correction with B. Clausii in breastfeed infants. Zaporozhye Medical Journal [Internet]. 2018May30 [cited 2024Nov.23];(3). Available from: http://zmj.zsmu.edu.ua/article/view/130489

Issue

No. 3 (2018)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)