Changes in the functional activity of thrombocytes in rats with experimental disruption of the periodontal connective tissue

Authors

  • V. F. Cheremisina National Pharmaceutical University, Kharkіv, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.4.135325

Keywords:

platelets, periodontitis, connective tissue

Abstract

 

Objective. The aim was to study the functional activity of platelets in periodontitis.

Materials and methods. Experiments were performed on 20 white nonlinear male rats weighing 320.0 ±30.0 g. Experimental periodontitis was modeled by the method of L. V. Peshkova. Blood for the study of platelet aggregation was taken by a syringe from the heart. Induced platelet aggregation was examined using a Computerized Platelet Aggregation Analyzer SOLAR 2110. Adenosine diphosphate (ADP) was used as an inducer at 2.5 μmol/L and 10.0 μmol/L concentration. The resulting aggregation was recorded at 37 °C for 10 min. Samples of periodontal tissues were fixed in a 10 % neutral-buffered formalin solution, dehydrated in an ascending concentration series of alcohol, followed by chloroform, chloroform-paraffin and embedded into paraffin. Sections 4–6 microns thick were cut on a sliding microtome and stained with hematoxylin-eosin and van Gieson’s stain.

Results. Analysis of aggregograms revealed a decrease in the functional activity of platelets in rats with experimental periodontitis at aggregation inducer concentrations of 2.5 μmol/L and 10.0 μmol/L. Reduction in the degree of platelets aggregation and aggregation rate were observed at an inductor concentration of 2.5 μmol/L in a group of rats with experimental periodontitis in comparison with the control. Microscopic examination showed a violation of the connective periodontal tissue histoarchitectonic. Dilatation of small capillaries in the connective tissue layers and in some preparations microvascular congestion were observed which indicated a violation of blood microcirculation, failure of repair regulatory mechanisms and the pathological process’s reaching the systemic level. The interaction of connective tissue cells with platelets played a significant role in these processes.

Conclusions. Decrease in thrombocytes aggregation activity is observed in rats with experimental periodontitis. Changes in the periodontal connective tissue manifested by the vascular pattern transformation, microcirculation violations, disruption of repair regulatory mechanisms and the pathological process’s reaching the systemic level.

References

Vygotskaya, Ya. I., Korol', Yu. S., & Buzherak, N. F. (2006). Priobretennye trombocitopatii [Acquired thrombocytopathy]. Mystetstvo líkuvannіa, 1, 23–25 [in Russian].

Serov, V. V, & Paukov, V. S. (Eds.). (1995). Vospalenie: Rukovodstvo dlya vrachej [Inflammation: a guide for doctors]. Moscow: Medicina [in Russian].

Peshkova, L. V. (1997). Spontannoe porazhenie tkanej parodonta u krys v usloviyahk vivariya, kak model' parodontita [Spontaneous destruction of periodontal tissues in vivarium conditions, as a model of periodontitis]. Vísnyk stomatolohíí, 2, 163–168. [in Russian].

Pavlov, S. B., Babenko, N. M., Kumechko, M. V., & Chernykh, L. V. (patentee) (2013). Patent 77372 Ukraina, MPK 601N 33/86 (2006.01). Sposib otsinky ahrehatsiinoi aktyvnosti trombotsytiv [Patent 77372 Ukraine, MPK 601N 33/86 (2006.01). A method for evaluating the aggregative activity of platelets]. Biuleten, 3, 3–4. [in Ukrainian].

(2001). Obschie e'ticheskie principy e'ksperimentov na zhivotnykh. Proceedings of the I National Congress on Bioethics. Kyiv: NANU. [in Russian].

Gubler, Ye. V., & Genkin, A. A. (1973). Primenenie neparametricheskikh kriteriev statistiki v mediko-biologicheskikh issledovaniyakh [Application of nonparametric statistical criteria in biomedical research]. Leningrad: Medicina. [in Russian].

Yagoda, A. V., & Koroi, P. V. (2002). Vliyanie interferona na agregatsiyu i sistemu ciklicheskikh nukleotidov trombocitov pri khronicheskikh virusnykh zabolevaniyakh pecheni [Effect of interferon-α on the thrombocyte aggregation and cyclic nucleotide system of patients with chronic viral hepatitis]. E'ksperimental'naya i klinicheskaya farmakologiya, 65(5), 46–49. [in Russian].

Downloads

How to Cite

1.
Cheremisina VF. Changes in the functional activity of thrombocytes in rats with experimental disruption of the periodontal connective tissue. Zaporozhye Medical Journal [Internet]. 2018Jul.13 [cited 2024Nov.23];(4). Available from: http://zmj.zsmu.edu.ua/article/view/135325

Issue

No. 4 (2018)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)