Indicators of phagocytic activity, autosensibilization and purine metabolism in patients with pneumoconiosis

Authors

  • S. S. Boieva Donetsk National Medical University, Kramatorsk, Ukraine,
  • О. A. Raksha-Sliusareva Donetsk National Medical University, Kramatorsk, Ukraine,
  • O. A. Sliusarev Donetsk National Medical University, Kramatorsk, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.4.135362

Keywords:

silicosis, pneumoconiosis, autoimmunity, phagocytosis, xanthine oxidase, uric acid

Abstract

The purpose of the work is to detect changes in the nonspecific component of resistance as well as to ascertain the probable presence of autoimmune reactions and disturbances of purine metabolism in miners of coal mines suffering from pneumoconiosis.

Materials and methods. 120 coal-miners with pneumoconiosis and 30 healthy miners were examined. The status of nonspecific body resistance was defined by indicators of phagocytic activity (phagocytes relative content, phagocytic number, phagocytic index), bactericidal activity in the spontaneous nitro blue tetrazolium test (NST-tests). The presence and severity of autoimmune processes were determined by the presence and level of circulating immune complexes, autosensibilization to lungs tissue, thymus, joint tissue, kidneys, nDNA and dDNA by passive haemaglutination test. Purine metabolism state was assessed by measuring of xanthine oxidase activity and uric acid level.

Results. Among coal-miners with pneumoconiosis the significant changes in the indicators of cellular and humoral immune responses have been revealed, in particular a reduction of spontaneous and reserve neutrophil phagocytic capacity as evidenced by marked and significant decrease in capable of ingesting microbial cells neutrophils relative count as well as phagocytic number and phagocytic index. Changes in neutrophils metabolism caused a decrease in neutrophil bactericidal activity in the spontaneous NST-tests and index of their activation; the development of autoimmune processes, which manifested as an increase in the blood serum content of circulating immune complexes, increased body sensibilization to its organs and even to nDNA and dDNA; an increase in xanthine oxidase activity and uric acid level.

Conclusions. The disturbances revealed demonstrated the mechanism of pneumoconiosis development among miners associated with the changes in purine metabolism and autoimmune reactions development against the background of nonspecific resistance system deficiency which reflected in a decrease of peripheral blood neutrophils absorbing and bactericidal capacity and an increase in circulating immune complexes level, which in turn resulted in inflammatory and autoimmune processes intensification.

 

References

Soriano, J.B., & Lamprecht, B. (2012). Chronic obstructive pulmonary disease: a worldwide problem. Med Clin North Am., 96(4), 671–680. doi: 10.1016/j.mcna.2012.02.005.

Shpagina, L. A., Artamonova, V. G., Fishman, B. B., Panacheva, L. A., Mazitova, N. N., Plyukhin, A. T., et al. (2015). Pnevmokoniozy. Klassyfikacija. Jepidemiologija. Patogenez. [Pneumoconiosis. Classification. Epidemiology. Pathogenesis]. Professional'nye zabolevaniya organov dykhaniya. (P. 363–488). Moscow: GEHOTAR-Media. [in Russian].

Nahorna, A. M., Vіtte, P. M., Sokolova, M. P., Kononova, I. G., Orekhova, O., & Mazur, V. V. (2012). Otsіnka ryzyky rozvytku profesіinykh zakhvoriuvan u pratsіvnykіv metalurgіinoi, vuhіlnoi promyslovostі ta mashynobuduvannіa Ukrainy [Assessment of risk development of occupational diseases in workers of metallurgic, mining industries and machine building Ukraine]. Ukrainskyi zhurnal z problem medytsyny pratsі, 3(31), 3–13. [in Ukrainian].

Jiang, Y., & Shao, F. (2013). A stone miner with both silicosis and constrictive pericarditis: case report and review of the literature. BMC Pulmonary Medicine, 13(1), 71. doi: 10.1186/1471-2466-13-71.

Nishimura, Y., Kumagai, N., Maeda, M., Hayashi, H., Fukuoka, K., Nakano, T., et al. (2011). Suppressive effect of asbestos on cytotoxicity of human NK cells. International Journal of Immunopathology and Pharmacology, 24(1), 5–10. doi: 10.1155/2012/492608.

Balamuralikrishnan, B., Balachandar, V., Subramaniam, M., Alagumuthu, K., Sureshkumar, S., Arun, M., et al. (2014). Assessment of genotoxic and humoral immune system alterations in silica exposed workers from pottery industries in South India. Stoch Environ Res Risk Assess, (28), 1801–1814. doi: 10.1007/s00477-013-0843-6.

De Capitani, E. M., Schweller, M., Silva, C. M., Metze, K., Cerqueira, E. M., & Bértolo, M. B. (2009). Rheumatoid pneumoconiosis (Caplan's syndrome) with a classical presentation. J. Bras Pneumol, 35(9), 942–946. doi: 10.1590/S1806-37132009000900017.

Il'derbaev, O.Z., Nurmukanov, D.K., & Il'derbaeva, G.O., D.Е. Usbekov (2015). Vliyanie ugol'noj pyli na aktivnost' fermentov cikla purinovyh nukleotidov v ehksperimente [Effect of coal dust on the activity of enzymes of the purine nucleotide cycle in the experiment]. Mezhdunarodnyj zhurnal ehksperimental'nogo obrazovaniya, (11-3), 457-459. [in Russian].

Shilova, L. N., Gontar, I. P., & Zborovskaja, I. A. (2011). Antitela k fermentam purinovogo metabolizma kak odin iz faktorov jendogennoj reguljacii jenzimaticheskoj aktivnosti pri sistemnoj sklerodermii [Antibodies to the enzymes of purine metabolism as one of the factors of endogenous regulation of enzymatic activity in the systemic scleroderma]. Vestnik SPbGU. Seriya 11. Medicina, (4), 42-47. [in Russian].

Men'shikov, V. V., Delektorskaja, L. N., & Zolotnickaja, R. P. (1987). Laboratornye metody issledovanija v klinike: sprav [Laboratory methods of research in the clinic: a reference book]. M. : Medicina. [in Russian].

Nagoev, B.S., & SHubich, M.G. (1981). Znachenie testa vosstanovleniya nitrosinego tetrazoliya dlya izucheniya funkcional'noj aktivnosti lejkocitov [Value of the test for the reduction of nitrosine tetrazolium for the studying of the functional activity of the leukocytes]. Laboratornoe delo, (4), 195-198. [in Russian].

Frimel', G., & Tarasova, A. (1987). Immunologicheskie metody [Immunological methods]: Medicina. [in Russian].

Karpishchenko, A. I. (2002). Spravochnik medicinskie laboratornye tekhnologii [Handbook: medical laboratory technologies]. SPb. : Intermedika, (Vols. 2). ISBN: 978-5-9704-2958-7 [in Russian].

Prisedskiy, Y. G. (1999). Statystychna obrobka rezultativ biolohichnykh eksperymentiv [Statistical processing of biological experiments results [Statistical processing of the results of biological experiments]. Kassіopeіa, Donetsk. [in Ukrainian].

How to Cite

1.
Boieva SS, Raksha-Sliusareva ОA, Sliusarev OA. Indicators of phagocytic activity, autosensibilization and purine metabolism in patients with pneumoconiosis. Zaporozhye Medical Journal [Internet]. 2018Jul.13 [cited 2024Nov.7];(4). Available from: http://zmj.zsmu.edu.ua/article/view/135362

Issue

Section

Original research