Single nucleotide polymorphisms of TLR-2, TLR-3, TLR-4 and susceptibility to inflammatory diseases of the respiratory tract

Authors

  • N. O. Pryimenko Higher State Educational Establishment of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • T. M. Kotelevska Higher State Educational Establishment of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • H. M. Dubynska Higher State Educational Establishment of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • I. P. Kaidashev Higher State Educational Establishment of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,
  • K. V. Pikul Higher State Educational Establishment of Ukraine “Ukrainian Medical Stomatological Academy”, Poltava,

DOI:

https://doi.org/10.14739/2310-1210.2018.5.141527

Keywords:

genotype, respiratory tract diseases, Arg753Gln polymorphism of TLR-2 gene, Leu412Phe of TLR-3 gene, Asp299Gly of TLR-4 gene

Abstract

The aim of the research is to establish an association between Arg753Gln polymorphism of TLR-2 gene, Leu412Phe of TLR-3 gene, Asp299Gly of TLR-4 gene and susceptibility to inflammatory diseases of the upper and lower respiratory tract and the complicated forms of АRI development.

Materials and methods. 98 healthy subjects distributed according to the genotype of TLR-2, TLR-3, TLR-4 were enrolled in the study of association between Arg753Gln polymorphism of TLR-2, Leu412Phe of TLR-3 and Asp299Gly of TLR-4 genes and inflammatory diseases of the upper and lower respiratory tract. The polymorphic site of TLR-2 Arg753Gln, TLR-3 Leu412Phe and TLR-4 Asp299Gly genotyping was performed by polymerase chain reaction using oligonucleotide primers. The relative risk of the disease and complications development was estimated using the odds ratio with 95 % confidence interval. The statistical significance of differences in qualitative characteristics was evaluated using Fischer's exact test.

Results. It has been revealed that individuals with polymorphic TLR-2, TLR-3 and TLR-4 genes have an increased susceptibility to АRI with frequent episodes during the year that are complicated by lower respiratory tract inflammation as well as chronic inflammatory diseases of the upper respiratory tract. It has been shown that the risk of bronchitis and pneumonia development in АRIs is higher in subjects with polymorphic genotypes of TLR-2, TLR-3 and TLR-4 as compared to the carriers of normal alleles distribution: 2.9 times with Leu/Phe genotype of TLR-3, 20.0 times with Phe/Phe of TLR-3 and 12.8 times with combinations of polymorphic genotypes of TLR-2, TLR-3, TLR-4.

Conclusions. The results of the study indicate that the presence of single-nucleotide polymorphisms TLR-2 Arg753Gln, TLR-3 Leu412Phe and TLR-4 Asp299Gly is the marker of high susceptibility to respiratory diseases. Individuals with polymorphic status of TLR-2, TLR-3 and TLR-4 genotypes have an increased susceptibility to inflammatory diseases of the upper and lower respiratory tract with АRI frequency of 4 or more episodes during the year.

 

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How to Cite

1.
Pryimenko NO, Kotelevska TM, Dubynska HM, Kaidashev IP, Pikul KV. Single nucleotide polymorphisms of TLR-2, TLR-3, TLR-4 and susceptibility to inflammatory diseases of the respiratory tract. Zaporozhye Medical Journal [Internet]. 2018Sep.24 [cited 2024Nov.5];(5). Available from: http://zmj.zsmu.edu.ua/article/view/141527

Issue

No. 5 (2018)

Section

Original research

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