Effectiveness of antimicrobial drugs against planktonic and biofilm forms of bacteria isolated from children with urinary tract infections

Authors

  • A. A. Vodianyk Bogomolets National Medical University, Kyiv, Ukraine,
  • Yе. O. Hrechukha Bogomolets National Medical University, Kyiv, Ukraine,
  • K. H. Lopatko National University of Life and Environmental Sciences of Ukraine, Kyiv,
  • V. A. Poniatovskyi Bogomolets National Medical University, Kyiv, Ukraine,
  • H. V. Hniloskurenko Bogomolets National Medical University, Kyiv, Ukraine,
  • I. O. Mitiuriaieva-Kornijko Bogomolets National Medical University, Kyiv, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.6.146191

Keywords:

biofilms, urinary tract infections, microbial drug resistance

Abstract

 

The aim of our work is to determine the susceptibility of clinical isolates of bacteria in biofilm to the most common antimicrobial drugs used in clinical practice.

Materials and methods. By means of serial dilutions of antimicrobial drugs, we analyzed the effectiveness of amoxicillin clavulanate, ceftriaxone, cefixime, gentamicin, furazidin and silver nanoparticles on planktonic and biofilm forms of 60 strains of bacteria isolated from children with lower urinary tract infections, acute and chronic pyelonephritis.

Results. Through the use of serial dilutions method it was found that 77 % of planktonic strains were susceptible to amoxicillin clavulanate, 70 % – to ceftriaxone, 47% – to cefixime, 78 % – to gentamicin and 92 % – to furazidin.

The percentage of the strains susceptible to amoxicillin clavulanate after biofilm formation was 63 %, to ceftriaxone – 17 %, to cefixime – 13 %, to gentamicin – 37 %, to furazidin – 80 %.

Conclusions. It was established that biofilm bacteria gain resistance to all the investigated drugs, but the change of susceptibility manifests itself in varying degrees.

The number of resistant strains of bacteria depends on the form of the urinary tract infection, in acute and chronic pyelonephritis the number of resistant strains is much greater than in infection of the lower urinary tract.

Planktonic and biofilm forms of bacteria were the most effectively influenced in vitro by furazidin, amoxicillin clavulanate and gentamicin. Cephalosporins (ceftriaxone, cefixime) were less effective against both forms of bacteria.

 

References

Butler, C. C., et al (2015). Childhood urinary tract infection in primary care: a prospective observational study of prevalence, diagnosis, treatment, and recovery. British Journal of General Practice. Apr; 65(633):e217-23.10.3399/bjgp15X684361

Centers for Disease Control and Prevention (CDC), (2013). Antibiotic Resistance Threats in the United States, https://www.cdc.gov. Retrieved from https://www.cdc.gov/drugresistance/threat-report-2013/index.html

Delcaru, C., et al (2016). Microbial Biofilms in Urinary Tract Infections and Prostatitis: Etiology, Pathogenicity, and Combating strategies. Pathogens. Dec; v. 5(4), 65. https://dx.doi.org/10.3390%2Fpathogens5040065

Dik, JWH., et al (2016). Cross-border comparison of antibiotic prescriptions among children and adolescents between the north of the Netherlands and the north-west of Germany. Antimicrobial Resistance and Infection Control. 5, 14.10.1186/s13756-016-0113-8

Di Martino, M., et al (2017). Prevalence of antibiotic prescription in pediatric outpatients in Italy: the role of local health districts and primary care physicians in determining variation. A multilevel design for healthcare decision support. BMC Public Health. Nov. 17, 17(1), 886. 10.1186/s12889-017-4905-4

European Centre for Disease Prevention and Control. Surveillance of antimicrobial resistance in Europe 2016. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net). Stockholm: ECDC.

Hay, AD., et al (2016). Improving the Diagnosis and Treatment of Urinary Tract Infection in Young Children in Primary Care: Results from the DUTY Prospective Diagnostic Cohort Study. Annals of Family Medicine. Jul; 14(4), 325–336.i: 10.1370/afm.1954

Macia, MD., et al (2014). Antimicrobial susceptibility testing in biofilm-growing bacteria. Clinical Microbiology and Infection Volume 20, Issue 10, October, 981-990. 10.1111/1469-0691.12651.

Shallcross, L., et al (2017). Antibiotic prescribing frequency amongst patients in primary care: a cohort study using electronic health records. J Antimicrob Chemother. Jun; 72(6), 1818–1824. : 10.1093/jac/dkx048

Soto, SM., et al (2014). Importance of Biofilms in Urinary Tract Infections: New Therapeutic Approaches. Advances in Biology. 13, 543974./10.1155/2014/543974

Downloads

How to Cite

1.
Vodianyk AA, Hrechukha YO, Lopatko KH, Poniatovskyi VA, Hniloskurenko HV, Mitiuriaieva-Kornijko IO. Effectiveness of antimicrobial drugs against planktonic and biofilm forms of bacteria isolated from children with urinary tract infections. Zaporozhye medical journal [Internet]. 2019Feb.1 [cited 2024Apr.25];(6). Available from: http://zmj.zsmu.edu.ua/article/view/146191

Issue

No. 6 (2018)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)