Pathomorphological changes in the periodontal complex tissues in the period of experimental bacterial-immune periodontitis chronization

Authors

  • A. Ye. Demkovych I. Horbachevsky Ternopil State Medical University, Ukraine,
  • Yu. I. Bondarenko I. Horbachevsky Ternopil State Medical University, Ukraine,
  • T. K. Нolovata I. Horbachevsky Ternopil State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.6.146196

Keywords:

periodontitis, inflammation, periodontium, cell proliferation, collagen

Abstract

Objective – to investigate pathomorphological changes in the periodontal complex on the 30th day of the experimental bacterial-immune periodontitis development.

Materials and methods. The study was conducted with the use of non-breeding clinically healthy male rats. Experimental bacterial-immune periodontitis in experimental animals was induced by injection of complex microbial mixture diluted with ovalbumin into periodontal tissue. Morphological analysis was used for estimation of the structural changes degree in the maxillofacial tissues. The mandible-related tissue fragments, in particular the periodontal complex material was embedded in the paraffin blocks. The cross sections of 5–6 microns thickness were made on a microtome. The preparations obtained were stained with hematoxylin and eosin.

Results. The high intensity of inflammatory infiltration was noted, although the progression of inflammatory and destructive processes was significantly slowed down. The histological examination showed that inflammatory process was characterized by signs of disorganization and destruction of the connective tissue and walls of the dental alveoli, the structural reconstruction of the gingival epithelium and its lamina propria. Inflammatory infiltration was accompanied by expressive signs of the connective tissue disorganization. At the same time there were morphological signs of reparative processes, proliferation and collagen fibers thickening. In this period granulation tissue was formed. At this stage inflammatory infiltration extended the crista of the cellular bone and penetrated the epithelium of dento-gingival junction and gingival sulcus.

Conclusions. The revealed pathomorphological changes in the periodontal complex tissues in the long-term period of the experimental bacterial-immune periodontitis are indicative of the inflammatory process chronization.

References

Slots, J. (2017). Periodontitis: facts, fallacies and the future. Periodontol. 2000, 75(1), 7–23. doi: 10.1111/prd.12221.

Di Benedetto, A., Gigante, I., Colucci, S., & Grano M. (2013). Periodontal disease: linking the primary inflammation to bone loss. Clin. Dev. Immunol., 2013, 503754. doi: 10.1155/2013/503754.

Chapple, I. L., Van der Weijden, F., Doerfer, C., Herrera, D., Shapira, L., Polak, D., et al. (2015). Primary prevention of periodontitis: managing gingivitis. J. Clin. Periodontol., 42(16), S71–6. doi: 10.1111/jcpe.12366.

Hodovana, O. (2015). Investigation of the level of mineral density of skeletal osseous tissue in patients with periodontal tissue diseases. Lik Sprava, 7–8, 123–128.

Hyde, S., Dupuis, V., Mariri, B. P., & Dartevelle, S. (2017). Prevention of tooth loss and dental pain for reducing the global burden of oral diseases. Int. Dent. J., 67(2), 19–25. doi: 10.1111/idj.12328.

Arimatsu, K., Yamada, H., Miyazawa, H., Minaqawa, T., Nakajima, M., Ryder, M. I., et al. (2014). Oral pathobiont induces systemic inflammation and metabolic changes associated with alteration of gut microbiota. Sci. Rep., 4, 4828. doi: 10.1038/srep04828.

Ioannidou, E. (2017). The Sex and Gender Intersection in Chronic Periodontitis. Front Public Health, 5, 189. doi: 10.3389/fpubh.2017.00189.

Dimitrova, A., & Kolenko, Y. (2013). Ocenka e'ffektivnosti razlichnykh immunomodulyatorov v kompleksnom lechenii generalizovanogo parodontita u lic molodogo vozrasta (18–25 let) [Estimation of efficiency of different immunomodulations in the holiatry of generalized paradontitis at the persons of young age (18–25 years)]. Sovremennaya stomatologiya, 2, 38–39. [in Russian].

Demkovych, A., Bondarenko, Yu., & Hasiuk, P. A. (2017). Oxidative modification of proteins in the process of experimental periodontitis development. Interventional Medicine and Applied Science, 9(4), 218–221. doi: [10.1556/1646.9.2017.28].

Sokos, D., Everts, V., & de Vries, T. J. (2015). Role of periodontal ligament fibroblasts in osteoclastogenesis: a review. J. Periodontal Res., 50(2), 152–159. doi: 10.1111/jre.12197.

Menicanin, D., Hynes, K., Han, J., Grothos, S., & Bartold, P. M. (2015). Cementum and Periodontal Ligament Regeneration. Adv. Exp. Med. Biol., 881, 207–236. doi: 10.1007/978-3-319-22345-2_12.

Bayani, M., Pourali, M., & Keivan, M. (2017). Possible interaction between visfatin, periodontal infection, and other systemic diseases: A brief review of literature. Eur. J. Dent., 11(3), 407–410. doi: 10.4103/ejd.ejd_284_16.

Livingstone, D., Murthy, V., Reddy, V. K., & Pillai, A. (2015). Prosthodontic rehabilitation of a patient with aggressive periodontitis. BMJ Case Rep., 2015. pii: bcr2014204588. doi: 10.1136/bcr-2014-204588.

Demkovych, A. Ye., & Bondarenko, Yu. I. (2015). Patohenetychni osnovy modelіuvannіa parodontytu u tvaryn [Pathogenetic basis of periodontitis modeling in animals]. Zdobutky klinichnoi i eksperymentalnoi medytsyny, 1(22), 54–57. [in Ukrainian].

Chan, J. K. (2014). The wonderful colors of the hematoxylin-eosin stain in diagnostic surgical pathology. Int. J. Surg. Pathol., 22(1), 12–32. doi: 10.1177/1066896913517939.

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1.
Demkovych AY, Bondarenko YI, Нolovata TK. Pathomorphological changes in the periodontal complex tissues in the period of experimental bacterial-immune periodontitis chronization. Zaporozhye Medical Journal [Internet]. 2019Feb.1 [cited 2024Nov.23];(6). Available from: http://zmj.zsmu.edu.ua/article/view/146196

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Original research