Changes in the system of asymmetric dimethylarginine - endothelial NO-synthase in patients with acute myocardial infarction and type 2 diabetes mellitus

Authors

  • D. V. Minukhina Kharkiv National Medical University, Ukraine,
  • V. D. Babadzhan Kharkiv National Medical University, Ukraine,
  • P. H. Kravchun Kharkiv National Medical University, Ukraine,
  • V. V. Minukhin Kharkiv National Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.6.146524

Keywords:

myocardial infarction, type 2 diabetes mellitus, asymmetric dimethylarginine, NO synthase, endothelium vascular, carbohydrate metabolism, lipid metabolism

Abstract

The purpose of this study was the assessment of asymmetric dimethylarginine (ADMA) and its links with carbohydrate and lipid metabolism parameters in patients with acute myocardial infarction (AMI) and type 2 diabetes mellitus following determination of asymmetric dimethylarginine and NO synthase plasma levels.

Materials and methods. The study included examination of 73 patients with acute myocardial infarction and type 2 diabetes mellitus, 57 patients with AMI without type 2 diabetes and 20 conditionally healthy individuals. The content of ADMA and NO synthase was measured by using an enzyme-linked immunosorbent assay kit (ADMA Xpress ELISA kit;Immundiagnostik,Austria) and Bender MedSystem (Austria), respectively. The parameters of carbohydrate and lipid metabolism were determined by a standard biochemical method.

Results. A significant 2.64 times increased level of ADMA (1.24 ± 0.04 μmol/L) was found in the patients with AMI and type 2 diabetes compared to the practically healthy persons (control group) (0.47 ± 0.05 μmol/L), and 2.53 times increased (P < 0.05) in comparison with the patients without type 2 diabetes. In the presence of type 2 diabetes the 40.1 % (P < 0.05) decrease in NOs level was noted compared to the control group (3.12 ± 0.11ng / ml and 5.21 ± 0.19 ng/ml, respectively) and the 26.1 % (P < 0.05) decrease compared to the patients with AMI without type 2 diabetes. The study provided a possibility to determine a direct correlation between ADMA and total cholesterol (r = 0.47; P < 0.05); LDL cholesterol (r = 0.28; P < 0.05), HDL cholesterol (r = -0.39; P < 0.05), triglycerides (P = 0.35; P < 0.05), glucose (P = 0.46; P < 0.05), insulin = 0.49; P < 0.05), glycosylated hemoglobin (r = 0.39; P < 0.05). Correlation analysis showed an inverse correlation between ADMA and NO synthase levels (r = -0.67; P < 0.05).

Conclusions. The association between asymmetric dimethylarginine high levels and pathological changes in the parameters of carbohydrate, lipid metabolism and endothelial dysfunction has been established, which increases ischemia in the vessel wall and adversely affects the course and prognosis of acute myocardial infarction in patients with type 2 diabetes mellitus.

 

 

References

Shumakov, V. A., Malinovskaya, I. E., Tereshkevyсh, L. P., Voloshina, O. V., Kryachok, T. A., Gotenko, I. A. & Kisilevych, L. F. (2014). Sakharnyj diabet u bol'nykh ostrym infarktom miokarda: kliniko-biokhimicheskie i funkcional'nye kharakteristiki [Diabetes mellitus in patients with acute myocardial infarction: clinical, biochemical and functional characteristics]. Ukrainskyi kardiolohichnyi zhurnal, 6, 28–32. [in Russian].

Yunzhou Dong, Yong Wu, Hyoung Chul Choi, and Shuangxi Wang (2016). Diabetic Endothelium Dysfunction, Cardiovascular Complications, and Therapeutics. Journal of Diabetes Research, 2016, 1–2. doi:10.1155/2016/5349801.

Bohlen, H. G. (2015). Nitric oxide and cardiovascular system. Compr Physiol., 5(2), 808–823. doi: 10.1002/cphy.c140052.

Gaiz, A., Mosawy, S., Colson, N., & Singh, I. (2017)..Thrombotic and cardiovascular risks in type two diabetes; Role of platelet hyperactivity. Biomed Pharmacother, 94, 679–686. doi: 10.1016/j.biopha.2017.07.121.

Tsikas, D., Bollenbach, A., Hanff, E., & Kayacelebi, A. A. (2018). Asymmetric dimethylarginine (ADMA), symmetric dimethylarginine (SDMA) and homoarginine (hArg): the ADMA, SDMA and hArg paradoxes. Cardiovasc Diabetol, 17(1), 1. doi: 10.1186/s12933-017-0656-x.

He, H., Wang, S., Li, X., Wang, H., Zhang, W., Yuan, L., & Liu, X. (2013). A novel metabolic balance model for describing the metabolic disruption of and interactions between cardiovascular-related markers during acute myocardial infarction. Metabolism, 62(10), 1357–1366. doi: 10.1016/j.metabol.2013.04.011.

(2014) Nakaz Ministerstva okhorony zdorovia Ukrainy «Unifikovanyi klinichnyi protokol ekstrenoi, pervynnoi, vtorynnoi (spetsializovanoi) ta tretynnoi (vysokospetsializovanoi) medychnoi dopomohy ta medychnoi reabilitatsii khvorykh na hostryi koronarnyi syndrom z elevatsiieiu sehmenta ST» vid 02.07.2014 r. №455 [Order of the Ministry of Health of Ukraine «Unified clinical protocol of emergency, primary, secondary (specialized) and tertiary (highly specialized) medical care and medical rehabilitation of patients with acute coronary syndrome with elevation of segment ST» from July 02, 2014 №455]. Kyiv.

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How to Cite

1.
Minukhina DV, Babadzhan VD, Kravchun PH, Minukhin VV. Changes in the system of asymmetric dimethylarginine - endothelial NO-synthase in patients with acute myocardial infarction and type 2 diabetes mellitus. Zaporozhye Medical Journal [Internet]. 2019Feb.1 [cited 2024Nov.23];(6). Available from: http://zmj.zsmu.edu.ua/article/view/146524

Issue

No. 6 (2018)

Section

Original research

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