Content of interleukins-4, 6 and nuclear transcription factor NF-κB in children with atopic bronchial asthma depending on Ile50Val polymorphism of IL4RA gene, severity of the disease course and level of its control
DOI:
https://doi.org/10.14739/2310-1210.2019.1.155818Keywords:
children, bronchial asthma, interleukins-4, 6, nuclear transcription factor (NF-kB), polymorphismAbstract
Many studies on polymorphic variants of cytokines genes indicate that their role in bronchial asthma development in children is still not fully understood.
Aim. To determine the indexes of interleukins – 4, 6 and nuclear transcription factor (NF-κB) content in patients with atopic bronchial asthma (BA) depending on Ile50Val polymorphism of IL4RA gene, severity of the disease course and level of its control.
Materials and methods. Alleles and genotypes were distributed according to the Ile50Val polymorphism of the IL4RA gene in 75 patients with atopic bronchial asthma and 25 healthy school-age children. Genotyping of the IL4RA gene was performed using a polymerase chain reaction (PCR). Biochemical studies on the interleukin-4 (IL-4), interleukin-6 (IL-6) levels and the content of nuclear factor-kappa B (NF-κB) determination were performed using ELISA kits.
The results of analysis revealed an increase in NF-κB level in patients with intermittent BA carrying A/A, A/G and G/G genotypes relative to IL-4 content: in the A/A carriers by 1.65 times (P < 0.05), A/G- by 1.45 (P < 0.05) and G/G – by 1.45 times (P < 0.05), and in comparison with the practically healthy children it was 2.3 times increased (P < 0.001). In persistent BA, the elevated level of IL-6 was observed only in the G/G carriers and it was 1.59 (P < 0.01) and 1.35 (P < 0.05) times higher than in A/A and A/G carriers, respectively. The level of IL-4 (4.91 ± 0.38 pg/ml) was 1.89 times higher (P < 0.05) in the A/A carriers with controlled BA than in practically healthy children, with partially controlled BA it exceeded by 2.35 times (6.10 ± 0.47 pg/ml, P < 0.05) and in uncontrolled BA the level of IL-4 was also increased, but differences were not significant (P > 0.05).
Conclusions. Thus, in children with BA and Ile50Val polymorphism of IL4RA gene, the activity of NF-κB has a significant effect and its content exceeds the levels of IL-4 and IL-6 in the genotypes A/A and A/G (P < 0.05) carriers, however, the NF-κB content in G/G carriers does not influence the level of BA control in children (P > 0.05).
References
Lytvynets, L. Ya. (2011). Kontseptsia dynamiky zmin pokaznykiv imunnoho statusu u ditei z bronkhialnoiu astmoiu zalezhno vid stupenia kontrolovanosti neduhy [The concept of the dynamics of changes in the immune status of children with bronchial asthma, depending on the degree of control of the disease]. Perynatolohiia i pediatriia, 2(46), 104–406. [in Ukrainian].
Okhotnikova, O. M., & Yakovleva, N. Yu. (2017). Genetychni aspekty alerhichnykh zakhvoriuvan [Genetic aspects of allergic diseases.Clinical Pediatrics]. Klinichna imunolohiia. Alerholohiia. Infektolohiia., 2(99), 61–66. [in Ukrainian].
Tyazhkaya, A. V., & Savenko, Yu. A. (2014). Prohhnozuvannia osoblyvostei klinichnoho perebihu bronkhialnoi astmy u ditei [Prediction of the features of the clinical course of asthma in children]. Sovremennaya pediatriya, 7(63), 120–123. [in Ukrainian]. doi: 10.15574/SP.2014.63.120.
Umanets, T. R. (2013). Kliniko-anamnestychni osoblyvosti fenotypiv bronkhialnoi astmy u ditei [Clinical and anamnestic features of phenotypes of bronchial asthma in children]. Perynatolohiia i pediatriia, 2(46), 69–71.[in Ukrainian].
Khobzei, M. K., Antypkin, Yu. H., Lapshyn, V. F., Umanets, T. R., Berezhnyi, V. V., Besh, L. V., et al. (2013) Unifikovanyi klinichnyi protocol pervynnoi, vtorynnoi (spetsializovanoi) medychnoi diahnostyky «Bronchialna astma u ditei» [Unified clinical protocol of primary, secondary (specialized) medical diagnostics. «Bronchial asthma in children»]. Kyiv. [in Ukrainian].
Freidin, M. B., Puzyrev,V. P., Ohorodova, L. M., & Brahina, O. Yu. (2013). Henetyka atopii: suchasnui stan [Genetics of Atopy: the present state]. Vesnik VOGiS, 3(10), 492–503. [in Ukrainian].
Chernyshova, O. Ye. (2016). Dyferentsiinyi pidkhid do likuvannia i vtorynnoi profilaktyky bronkhialnoi astmy na tli persystuiuchoi infektsii u ditei [Differential approach to the treatment and secondary prevention of bronchial asthma against the background of persistent infections in children.]. Zdorov'e rebenka, 8(76), 24–55. [in Ukrainian].
Global strategy for the diagnosis and management of asthma in children 5 years and younger. Retrieved from http: //www.ginasthma/ org / pdf / GINA_ Report_2017 pdf.
Idzerda, R. L., March, C. J., Mosley, B., Lyman, S. D., Vanden Bos, T. V., Gimpel, S. D., et al. (1990). Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J. Exp. Med, 171(3), 861–873.
Mitsuyasu, H., Izuhara, K., Mao, X.-Q., Gao, P.-S., Arinobu, Y., Enomoto, T., et al. (1998). Ile50Val variant of IL4Rα upregulatesIgE synthesis and associates with atopic asthma. Nature Genetics, 19(2), 119–120. doi: 10.1038/472
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
