Content of interleukins-4, 6 and nuclear transcription factor NF-κB in children with atopic bronchial asthma depending on Ile50Val polymorphism of IL4RA gene, severity of the disease course and level of its control
DOI:
https://doi.org/10.14739/2310-1210.2019.1.155818Keywords:
children, bronchial asthma, interleukins-4, 6, nuclear transcription factor (NF-kB), polymorphismAbstract
Many studies on polymorphic variants of cytokines genes indicate that their role in bronchial asthma development in children is still not fully understood.
Aim. To determine the indexes of interleukins – 4, 6 and nuclear transcription factor (NF-κB) content in patients with atopic bronchial asthma (BA) depending on Ile50Val polymorphism of IL4RA gene, severity of the disease course and level of its control.
Materials and methods. Alleles and genotypes were distributed according to the Ile50Val polymorphism of the IL4RA gene in 75 patients with atopic bronchial asthma and 25 healthy school-age children. Genotyping of the IL4RA gene was performed using a polymerase chain reaction (PCR). Biochemical studies on the interleukin-4 (IL-4), interleukin-6 (IL-6) levels and the content of nuclear factor-kappa B (NF-κB) determination were performed using ELISA kits.
The results of analysis revealed an increase in NF-κB level in patients with intermittent BA carrying A/A, A/G and G/G genotypes relative to IL-4 content: in the A/A carriers by 1.65 times (P < 0.05), A/G- by 1.45 (P < 0.05) and G/G – by 1.45 times (P < 0.05), and in comparison with the practically healthy children it was 2.3 times increased (P < 0.001). In persistent BA, the elevated level of IL-6 was observed only in the G/G carriers and it was 1.59 (P < 0.01) and 1.35 (P < 0.05) times higher than in A/A and A/G carriers, respectively. The level of IL-4 (4.91 ± 0.38 pg/ml) was 1.89 times higher (P < 0.05) in the A/A carriers with controlled BA than in practically healthy children, with partially controlled BA it exceeded by 2.35 times (6.10 ± 0.47 pg/ml, P < 0.05) and in uncontrolled BA the level of IL-4 was also increased, but differences were not significant (P > 0.05).
Conclusions. Thus, in children with BA and Ile50Val polymorphism of IL4RA gene, the activity of NF-κB has a significant effect and its content exceeds the levels of IL-4 and IL-6 in the genotypes A/A and A/G (P < 0.05) carriers, however, the NF-κB content in G/G carriers does not influence the level of BA control in children (P > 0.05).
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