Investigations of the antimicrobial activity of aminomethanesulfonic acids against strains of Staphylococcus aureus with different antimicrobial susceptibility

Authors

  • T. L. Hrydina Odesa National Medical University, Ukraine,
  • R. Ye. Khoma Physical-Chemical Institute for Environment and Human Protection of MES of Ukraine and NAS of Ukraine, Odesa,
  • A. A.-A. Ennan Physical-Chemical Institute for Environment and Human Protection of MES of Ukraine and NAS of Ukraine, Odesa,
  • A. S. Fedchuk Physical-Chemical Institute for Environment and Human Protection of MES of Ukraine and NAS of Ukraine, Odesa,
  • O. A. Hruzevskyi Odesa National Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2019.2.161502

Keywords:

aminomethanesulfonic acids, antimicrobial activity, Staphylococcus aureus

Abstract

One of the tasks of the WHO strategy against development of antibiotic resistance in microorganisms is the searching for new compounds with antimicrobial activity to develop new antimicrobial medicines.

The aim of this study was to determine the inhibitory effect of aminosulfonic acid (AMSA) and its new derivatives such as N-methyl-(MeAMSA), N-(2-hydroxyethyl)-(HEAMSA), N-benzyl-(BnAMSA), N-(tert-butyl)-(t-BuAMSA), 4-(N-phenylaminomethyl)phenyl (PhAMPhAMSA) on the growth of Staphylococcus aureus strains with different antimicrobial susceptibility.

Materials and methods. The method of serial dilution was used in the study. The chemical compounds were dissolved in DMSO (a final concentration of 1%). Then dilutions of the compounds were performed using liquid Mueller-Hinton medium to final concentrations of 5 mM and 10 m×M. The results were assessed using a Densi-La-Meter after 18-20 hours of incubation at 37 °C. Sulfanilamide was used as a reference preparation.

Results. AMSA suppressed the growth of all tested strains regardless of their antibiotic resistance profiles even more than sulfanilamide. MeAMSA inhibited the growth of S. aureus ATCC 25923 and S. aureus 2781 strains more than the reference preparation, but less than AMSA. No inhibitory effect was observed on the antibiotic resistant S. aureus Kunda strain. Compounds of HEAMSA, t-BuAMSA, BnAMSA stably inhibited the growth of all strains tested. These compounds suppressed the growth of S. aureus ATCC 25923 and S. aureus Kunda strains more than sulfanilamide. However, the reference preparation exhibited greater S. aureus 2781 growth inhibition than investigated preparations. Compound PhAMPhAMSA did not show antimicrobial activity.

Conclusions. Aminomethanesulfonic acid derivatives suppressed the growth of Staphylococcus aureus strains with different antimicrobial susceptibility and their antimicrobial activity was higher than that of sulfanilamide. Further study of these compounds efficacy on different types of microorganisms can be considered promising for the development of new antimicrobial agents. It would also be appropriate to study the combined use of these substances with antibiotics.

 

References

(2018) Antimicrobial resistance. WHO Informational bulletin. Fact sheet Updated Retrieved from http://www.who.int/mediacentre/factsheets/fs194/en/

WHO (2016) Global action plan on antimicrobial resistance. Retrieved from http://apps.who.int/iris/bitstream/10665/254884/1/9789244509760-rus.pdf?ua=1

Para, R. A., Fomda, B. A., Jan, R. A., Shah, S., & Koul, P. A. (2018). Microbial etiology in hospitalized North Indian adults with community-acquired pneumonia. Lung India, 35(2), 108–115. doi: 10.4103/lungindia.lungindia_288_17.

Ashraf S., Chaudhry U., Raza A., Ghosh D., Zhao X. (2018). In vitro activity of ivermectin against Staphylococcus aureus clinical isolates. Antimicrob Resist Infect Control., 7(1), 27. doi: 10.1186/s13756-018-0314-4

Guay, I., Boulanger, S., Isabelle, C., Brouillette, E., Chagnon, F., Bouarab, K., et al. (2018). Tomatidine and analog FC04-100 possess bactericidal activities against Listeria, Bacillus and Staphylococcus spp. BMC Pharmacol Toxicol., 19(1), 7. doi: 10.1186/s40360-018-0197-2.

Wilson, T. J., Blackledge, M. S., & Vigueira, P. A. (2018). Resensitization of methicillin-resistant Staphylococcus aureus by amoxapine, an FDA-approved antidepressant. Heliyon, 4(1), e00501. doi: 10.1016/j.heliyon.2017.e00501.

Shahzad, S., Ashraf, M. A., Sajid, M., Shahzad, A., Rafique, A., & Mahmood, M. S. (2018). Evaluation of synergistic antimicrobial effect of vitamins (A, B1, B2, B6, B12, C, D, E and K) with antibiotics against resistant bacterial strains. J. Glob Antimicrob. Resist., 13, 231–236. doi: 10.1016/j.jgar.2018.01.005

Ferreira, C. M. H., Pinto, I. S. S., Soares, E. V., & Soares, H. M. V. M. (2015). (Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a review. RSC Adv., 5(3), 30989–31003. doi: 10.1039/C4RA15453C

Stacenko, M. E., Vinnikova, A. A., Ronskaya, A. M., & Shilina, N. N. (2013). Taurin v terapii khronicheskoj serdechnoj nedostatochnosti i sakharnogo diabeta 2 tipa: vliyanie na mikrocerkulyaciyu і e'lasticheskie svojstva magistral'nykh sosudov [Taurine in the therapy of chronic heart failure and type 2 diabetes mellitus: the effect on microcirculation and elastic properties of the main vessels]. Serdechnaya nedostatochnost', 14, 6(80), 347–353. [in Russian].

Marcinkiewicz, J., & Kontny, E. (2014). Taurine and inflammatory diseases. Amino Acids, 46(1), 7–20. doi: 10.1007/s00726-012-1361-4.

Ye, H.-B., Shi, H.-B., & Yin, S.-K. (2013). Mechanisms Underlying Taurine Protection Against Glutamate-Induced. Neurotoxicity. Can. J. Neurological Sci., 40(5), 628–634. doi: 10.1017/S0317167100014840

Strus, M., Walczewska, M., Machul, A., Mikołajczyk, D., & Marcinkiewicz, J. (2015) Taurine Haloamines and Biofilm. Part I: Antimicrobial Activity of Taurine Bromamine and Chlorhexidine Against Biofilm Forming Pseudomonas aeruginosa. Taurine 9. Advances in Experimental Medicine and Biology, 803, 121–132. doi: 10.1007/978-3-319-15126-7_11.

Khoma, R. E., Shestaka, A. A., Shishkin, O. V., Baumer, V. N., Brusilovskii, Yu. E., Koroeva, L. V., et al. (2011) Features of interaction in the sulfur(IV) oxide-hexamethylenetetramine-water system: A first example of identification of the product with a sulfur-carbon bond. Russ. J. Gen. Chem., 81(3), 620–621. doi: 10.1134/S1070363211030352

Khoma, R. E., Gemboldt, V. O., Shishkin, O. V., Baumer, V. N., & Koroeva, L. V. (2013) Synthesis, crystal structure, and spectral characteristics of N-(Hydroxyethyl)aminomethanesulfonic acid. Russ. J. Gen. Chem., 83(5), 969–971. doi: 10.1134/S1070363213050149.

Balouiri, М., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal., 6(2), 71–79. doi: 10.1016/j.jpha.2015.11.005.

Becheker, I., Berredjem, H., Boutefnouchet, N., Berredjem, M., & Ladjama, A. (2014) Antibacterial activity of four sulfonamide derivatives against multidrug-resistant Staphylococcus aureus. J. Chem. Pharm. Res., 6(11), 893–899.

How to Cite

1.
Hrydina TL, Khoma RY, Ennan AA-A, Fedchuk AS, Hruzevskyi OA. Investigations of the antimicrobial activity of aminomethanesulfonic acids against strains of Staphylococcus aureus with different antimicrobial susceptibility. Zaporozhye medical journal [Internet]. 2019Apr.2 [cited 2024Mar.28];(2). Available from: http://zmj.zsmu.edu.ua/article/view/161502

Issue

Section

Original research