A novel wound dressing material with prolonged action

Authors

  • S. M. Dronov SI “Dnipropetrovsk Medical Academy оf Ministry of Health of Ukraine”, Dnipro,
  • V. Yo. Mamchur SI “Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine”, Dnipro,
  • I. P. Koshova SI “Dnipropetrovsk Medical Academy оf the Ministry of Health of Ukraine”, Dnipro,
  • D. O. Stepanskyi SI “Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine”, Dnipro,
  • H. M. Kremenchutskyi SI “Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine”, Dnipro,
  • V. M. Toropin Tax and Customs Inspection Department of the SFS of Ukraine, Dnipro,
  • B. V. Murashevych State Higher Educational Institution "Ukrainian State University of Chemical Technology", Dnipro,
  • K. S. Burmistrov State Higher Educational Institution "Ukrainian State University of Chemical Technology", Dnipro,

DOI:

https://doi.org/10.14739/2310-1210.2019.3.169189

Keywords:

immobilized fibrous sodium N-chlorosulfonamide, N, N-dichlorosulfonamide, dressing, toxicity, anti-infective agents

Abstract

 

The development and creation of non-toxic dressing materials exhibiting pronounced antimicrobial effect and accelerating wound-healing process without increasing pathogenic microorganisms drug resistance is an actual task of modern medicine.

Aim. Evaluation of toxicological characteristics of a dressing material containing 6 % and 9 % active chlorine in a single application on the skin as well as the study of dressing material effect on the epithelization rate of the uninfected wound surface and determination of its antimicrobial and wound healing activity in vivo on a model of S. aureus ATCC 6538-infected wound.

Materials and methods. An experimental study of the dressings with immobilized polymeric N-chlorosulfonamide sodium and N,N-dichlorosulfonamide was performed to evaluate the safety in use and pharmacological activity in vivo. Evaluation of the dressing material toxicological characteristics in a single application on the skin was carried out on laboratory rats and rabbits according to the generally accepted method, the wound healing effect was studied using the test by L. N. Popova and antimicrobial activity in vivo - on a model of wound infected by the insertion of a 24-hour culture of S. aureus ATCC 6538 contaminated silk thread through the skin.

Results. Acute toxicity pharmacological studies of material test-samples containing 6 % and 9 % active chlorine in a single application on the skin of rats and rabbits found that these applications did not lead to deaths, as well as changes in their behavioral pattern, consumption of feed and water. The epithelization rate of uninfected wound surface in application of dressings containing both sodium N-chlorosulfonamide and N, N-dichlorosulfonamide was significantly, on average 1.5 times, faster at the end of the observational period (day 14) in comparison with the control group animals. According to the data obtained, the application of dressings containing sodium N-chlorosulfonamide on the 24-hour culture of S. aureus ATCC 6538 infected wound significantly reduced the intensity of the inflammatory process local manifestations and 4–10 times decreased the number of pathogenic staphylococci under bandages on day 8 of observation.

Conclusions. Thus, a single application of native materials on the animal skin does not cause a lethal outcome. Skin application of polymeric materials on uninfected wounds stimulates the processes of natural reparation, and decreases the number of pathogenic microorganisms in wound fluid as well as shortens the healing time in staphylococcus-infected wounds.

 

 

References

Doyle, J. W., Roth, N. P., Smith, R. M., Li, Y. Q., & Dunn, R. M. (1996). Effects of calcium alginate on cellular wound healing processes modeled in vitro J. Biomed. Mater. Res, 32(4), 561–568. doi: 10.1002/(SICI)1097-4636(199612)32:4<561::AID-JBM9>3.0.CO;2-P

Mіsiura, V. M., & Roik, O. M. (2014). Poviazky helevі antymіkrobnі «OpіkUn» – suchasnyi pereviazuvalnyi zasіb lіkuvannia ran і opіkіv [Gel antimicrobial bandages "OpikUn" - a modern dressing wound and wound treatment]. Ekstrenna medytsyna vіd nauky do praktyky, 5–6(11), 232–233. [in Ukrainian].

Sutkovskaya, Yu. V. (2016). Prolezhni i troficheskie yazvy: poyavilas’ li nadezhda [Bedsores and trophic ulcers: did hope appear]. Uchastkovyj vrach, 3(44), 38–39. [in Russian].

Stewart, P. S. (2001). Antibiotic resistance of bacteria in biofilms. Lancet, 358(9276), 135–8.

Weiss, J., Klein, R., Slivka, A., & Wei, M. (1982). Chlorination of taurine by human neutrophils. J.Clin.Invest, 70(3), 598–607.

White’s Handbook of Chlorination and Alternative Disinfectants (2011). New Jersey: John Wiley and Sons.

Dakin, H. D. (1915). On the use of certain antiseptic substances in the treatment of infected wounds. Br.Med. J., 2, 318–320.

Abaev, Yu. K. (2003). Wound dressings in surgery. Medicinskie novosti, 12, 30–37.

Crew, J. R., Varilla, R., Rocas, T. A., Debabov, D., Wang, L., Najafi, A., et al. (2012). NeutroPhase® in chronic non-healing wounds Int. J. Burn Trauma, 2(3), 126–134.

Crew, J. R., Thibodeaux, K. T., Speyrer, M. S., Gauto, A. R., Shiau, T., Pang, L., et al. (2016). Flow-through Instillation of Hypochlorous Acid in the Treatment of Necrotizing Fasciitis. Wounds, 28(2), 40–47.

Burmіstrov, K. S., Murashevych, B. V., Toropіn, V. M., & Toropіn, M. V. (2016) Pat. 112187 Ukraina. A01R 1/00, C08F 257/02, S08F 259/02. Polіmernyi materіal z іmmobіlіzovanym «aktyvnym khlorom», scho proiavliaie antymіkrobnі vlastyvostі [Patent №112187 Ukraine Polymeric material with immobilized "active chlorine" exhibiting antimicrobial properties]. Biuleten, 23. [in Ukrainian].

Soldatov, V., Pawlowski, L. A., & Shunkevich, H. (2004) Wasag New materials and technologies for environmental engineering: synthesis and structure of ion exchange fibers. Lublin: Liber Duo Color.

Burmistrov, K. S., Toropin, V. N., Kremenchutskyy, G. N., Polikarpov, A. P., & Shunkevich, A. A. (2016). Novyj hlorvydelyayushhij material shirokogo naznacheniya. Stroenie i svojstva [New material chloro allocates wide application: structure and properties]. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj khimii, 12(19), 10–14.

Toropin, V. N., Surmasheva, E. V., & Romanenko, L. I. (2016). Izuchenie antimikrobnykh svojstv immobilizovannykh voloknistykh N- chlorosul'fonamidov [The study of antimicrobial properties of immobilized fi brous N-chloro sulfonamides]. Current issues in pharmacy and medicine: science and practice, 3, 54–58. doi: 10.14739/2409-2932.2016.3.77993 [in Russian].

Toropin, V. N., Burmistrov, K. S., Surmasheva, E. V, Romanenko, L. I. (2016). Izuchenie antimikrobnyh svojstv immobilizovannykh voloknistykh N,N-dikhlorsul’fonamidov [Study of the antimicrobial properties of immobilized fibrous N,N-dichlorosulfonamides]. Science Rise: Pharmaceutical Science, 4(4), 48–52. doi: 10.15587/2519-4852.2016.85905 [in Russian].

Surmasheva, O. V., Romanenko, L. І., Kremenchutskyi H. M., Stepanskii, D. O., Koshova, І. P., Toropіn, V. M., & Murashevich, B. V. (2017) Vyvchennia antymіkrobnykh vlastyvostei іmmobіlіzovanykh voloknystykh form N-hlor- ta N,N-dikhlorsulfonamіdіv [Study of antimicrobial properties of immobilized fibrous forms of N-chlorine N, N-dichlorosulfonamides]. Zdobutky klіnіchnoi ta eksperymentalnoi medyctsyny Proceedings of the final Scientific and Practical Conference (р. 358–360). [in Ukrainian].

(1986). European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Stientific Purposes. Strasburg: Council of Europe.

Sidorov, K. K. (1976). Vvedenie veshchestv v zheludok, v trakheyu, pod kozhu, v venu i drugie puti vvedenyia yadov laboratornym zhyvotnym [Introduction of substances into the stomach, into the trachea, under the skin, into a vein and other routes of administration of poisons to laboratory animals]. Metody opredeleniya toksichnosti i opasnosti khimicheskikh veshhestv. Moscow: Medicina. [in Russian].

Efimov, E. A. (1999). Faktory, vliyayuschie na polnotu regeneracii kozhi u mlekopitayuschih [Factors affecting the completeness of skin regeneration in mammals]. Izvestiya RAN. Seriya: Biologiya, 4, 488–492. [in Russian].

Mazur, I. A., Mamchur, V. I., Podpletnyaya, E. A., et al. (patentee) (2008) Patent №2317818, RU. MPK A61K31/41, A61K31/165. Ranozazhivlyayuschee i protivomikrobnoe sredstvo [Patent. №2317818. Wound healing and antimicrobial agent]. [in Russian].

Prikaz MOZ SSSR «Ob unifikacii mikrobiologicheskikh (bakteriologicheskikh) metodov issledovaniya, primenyaemykh v kliniko-diagnosticheskikh laboratoriyakh lechebno-profilakticheskikh uchrezhdeniyakh» ot 22.04.1985 №535 [Order of the Ministry of Health of the USSR «On the unification of microbiological (bacteriological) research methods used in clinical diagnostic laboratories for treatment and prevention institutions» from 22.04.1985 №535]. [in Russian].

How to Cite

1.
Dronov SM, Mamchur VY, Koshova IP, Stepanskyi DO, Kremenchutskyi HM, Toropin VM, Murashevych BV, Burmistrov KS. A novel wound dressing material with prolonged action. Zaporozhye Medical Journal [Internet]. 2019May31 [cited 2024Nov.23];(3). Available from: http://zmj.zsmu.edu.ua/article/view/169189

Issue

Section

Original research