Changes in T-helper associated cytokines and the risk of miscarriage
DOI:
https://doi.org/10.14739/2310-1210.2019.3.169191Keywords:
pregnancy, spontaneous abortion, immune system, cytokinesAbstract
Objective: to study the cytokine profile features of women in the dynamics of pregnancy complicated by threatened miscarriage during the first trimester.
Materials and methods. The analysis of pregnancy and childbirth course of 294 primigravida women was carried out and 72 women whose pregnancy was complicated by threatened miscarriage during the first trimester were enrolled. The control group included 48 somatically healthy pregnant women with a physiological course of pregnancy. Serum cytokines concentrations (interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10), g-interferon (IFNg), tumor necrosis factor-a (TNFa)) were determined by ELISA using a photometer "Digi Scan-400" (Austria) in the clinical groups of pregnant woman during the first (10–14 weeks), second (23–26 weeks) and third trimesters (32–35 weeks).
Results. In women with threatened miscarriage during the first trimester, characteristic changes in the cytokine profile were revealed, which can be divided into 2 groups: the 1st group – changes associated with the functional activity of Т-helper type 1(Th1) (decreased IL-1β and increased TNFa levels during the first trimester; decreased level of TNFa during the second trimester); the 2nd group – changes associated with functional activity of Т-helper type 2 (Th2) (increased levels of IL-4 and IL-10 during the second trimester of pregnancy).
Conclusions. The dynamics of pregnancy complicated by threatened miscarriage during the first trimester involve changes in the character of cytokine profile represented by Th1 cells activation during the first trimester followed by their function depression during the second trimester on the background of increasing production of anti-inflammatory cytokines by Th2 cells. During the third trimester, inflammatory and anti-inflammatory cytokines production is decreased on the background of increased macrophage activity with elevated TNFa level in the blood serum of women whose pregnancy was complicated by threatened miscarriage during the first trimester.
References
Batrak, N., Malyshkina, A., & Kroshkina, N. (2014). Immunologicheskie aspekty privychnogo nevynashivaniya beremennosti [Recurrent miscarriage: Immunological aspects]. Akusherstvo i ginekologiya, 12, 10–14. [in Russian].
Aganezov, S., & Aganezova, N. (2015). Vozmozhnosti snizheniya riska prezhdevremennykh rodov s pozicii dokazatel'noj mediciny [Possibilities for reducing the risk of preterm birth in the context of evidence-based medicine]. Akusherstvo i ginekologiya, 4, 62–68. [in Russian].
Carlson, S. E., Gajewski, B. J., Valentine, C. J., Rogers, L. K., Weiner, C. P., DeFranco, E. A., & Buhimschi, C. S. (2017). Assessment of DHA on reducing early preterm birth: the ADORE randomized controlled trial protocol. BMC pregnancy and childbirth, 17(1), 62. doi: 10.1186/s12884-017-1244-5
(2018). World Health Organisation.Preterm birth: Key facts. Retrived from http://www.who.int/en/news-room/fact-sheets/detail/preterm-birth
Vorobiova, I. I., Skrypchenko, N. Ya., Livshyts, L. A., Zhyvetska-Denysova, A. A., Pysareva, S. P., Tkachenko, V. B., et al. (2016). Imuno-henetychni pidkhody do diahnostyky nevynoshuvannia vahitnosti yak multyfaktorialnoho zakhvoryuvannia [Immuno-genetic approaches to the diagnostics of miscarriage as multifactorial diseases]. Кyiv. [in Ukrainian].
Dobrokhotova, Yu. Ye., Gankovskaya, L. V., Bakhareva, I. V., Svitich, O. A., Malushenko, S. V., & Magomedova, A. M. (2016). Rol´ immunnykh mekhanizmov v patogeneze nevynashivaniya beremennosti [The role of immune mechanisms in the pathogenesis of miscarriages]. Akusherstvo i ginekologiya, 7, 5–10. [in Russian]. https://dx.doi.org/10.18565/aig.2016.7.5-10
Köstlin, N., Ostermeir, A. L., Spring, B., Schwarz, J., Marmé, A., Walter, C. B., et al. (2017) HLA-G promotes myeloid-derived suppressor cell accumulation and suppressive activity during human pregnancy through engagement of the receptor ILT4. Eur J Immunol., 47(2), 374–384. doi: 10.1002/eji.201646564
Lee, C. L., Guo, Y., So, K. H., Vijayan, M., Guo, Y., Wong, V. H., et al. (2015) Soluble human leukocyte antigen G5 polarizes differentiation of macrophages toward a decidual macrophage-like phenotype. Hum Reprod., 30(10), 2263–74. doi: 10.1093/humrep/dev196
Lebedeva, O., P., Ivashova, O. N., Starceva, N. Y., Pakhomov, S. P., & Churnosov, M. I. (2015). Signalnye receptory vrozhdennogo immuniteta v indukcii apoptoza pri nevynashivanii beremennosti rannikh srokov [Innate immunity signaling receptors in the induction of apoptosis in early miscarriage]. Akusherstvo i ginekologiya, 2, 39–43. [In Russian].
Khachatryan, A. M., Krechetova, L. V., & Tetruashvili, N. K. (2014). Alloimmunnye mekhanizmy privychnogo vykidysha [Alloimmune mechanisms of recurrent miscarriage]. Akusherstvo i ginekologiya, 5, 3–8 [in Russian].
Chernobai, L. V., Tyshchenko, O. M., Lazurenko, V. V., Muryzina, I. Yu., Yurkova, O. V. (2016). Rol´ narusheniya citokinovogo profilya v snizhenii fertil´nosti [The role of citokyn profile isorders in fertility reduction]. Mízhnarodnyi medychnnyi zhurnal, 2, 38–43. [in Russian].
Chatterjee, P., Chiasson, V. L., Bounds, K. R., & Mitchell, B. M. (2014) Regulation of the anti-inflammatory cytokines interleukin-4 and interleukin-10 during. Front. Immunol., 5, 253. doi: 10.3389/fimmu.2014.00253.
Young, O. M., Tang, Z., Niven-Fairchild, T., Tadesse, S., Krikun, G., Norwitz, E. R., et al. (2015) Toll-like receptor-mediated responses by placental Hofbauer cells (HBCs): a potential pro-inflammatory role for fetal M2 macrophages. Am J Reprod Immunol., 73(1), 22–35. doi: 10.1111/aji.12336
Ferstl, R., Frei, R., Barcik, W., Schiavi, E., Wanke, K., Ziegler, M., et al. (2017) Histamine receptor 2 modifies iNKT cell activity within the inflamed lung, Allergy, 72(12), 1925–1935. doi: 10.1111/all.13227
Sykes, L., Macintyre, D. A., Yap, X. J., Teoh, T.G., & Bennett, P. R. (2012) The Th1:Th2 dichotomy of pregnancy and preterm labour. Mediators Inflamm., 2012(2), 967629. doi: 10.1155/2012/967629
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