Relationship between the expression level of microRNA and the features of clinical course of the gallbladder and Оddi's sphincter functional disorders in children
DOI:
https://doi.org/10.14739/2310-1210.2019.5.179434Keywords:
biliary dyskinesia, Oddi's sphincter dysfunction, genetic epigenesis, microRNA, childrenAbstract
The aim of the work is to establish the relationship between the expression levels of microRNA-378f, microRNA-4311, microRNA-4714-3p and anamnestic, clinical and paraclinical parameters of functional disorders of the gallbladder and Oddi's sphincter (FD GB and OS) in children.
Materials and methods. We examined 50 children with FD GB and OS aged from 4 to 14 years old. The control group included 20 healthy children of the same age. All the children, in addition to general clinical examination, underwent a molecular genetic study with the determination of the expression level of microRNA-378f, microRNA-4311, microRNA-4714-3p in blood serum by the method of real-time reverse transcription polymerase chain reaction according to TaqMan Gene Expression Assays protocol. In total, 140 clinical, laboratory and instrumental diagnostic indicators of 50 patients with verified FD GB and OS were analyzed using correlation analysis.
Results. Associations between the expression level of microRNA-378f and familial heredity of the biliary tract diseases with FD GB and OS in mothers (r = +0.27; P < 0.05) and sisters (r = +0.39; P < 0.05) of patients were identified. A positive correlation was registered between the expression levels of microRNA-378f and micro-RNA-4311 and FD GB and OS in maternal relatives (r = +0.35 and r = +0.32, respectively; P < 0.05). Associations between the expression levels of microRNA-4311 and microRNA-4714-3p and burdened allergic history in children with FD GB and OS (r = +0.41; r = +0.37, respectively; P < 0.05) were established. Analysis of the correlation diagram showed that the expression level of microRNA-4714-3p was positively correlated with a decrease in the gallbladder contractility (r = +0.36; P < 0.05).
Conclusions. Significant correlations between the expression levels of microRNA-378f and microRNA-4311 and familial aggregation of the biliary tract pathology in the pedigrees of children with FD GB and OS were established. It was determined that the burdened allergic history was associated with the expression levels of microRNA-4311 and micro-RNA4714-3p in the examined children. Associations between the expression levels of microRNA-4311 and microRNA-4714-3p and clinical manifestations of abdominal pain syndrome, dyspeptic and asteno-vegetative syndromes of FD GB and OS in children were found. It was revealed that a decrease in the expression level of microRNA-4714-3p was associated with a decrease in the gallbladder contractility in children with FD GB and OS.
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