Characteristics of interleukin-4 gene (C-589T, rs2243250) polymorphism in children with bronchial asthma and allergic rhinitis with isolated or allergic rhinitis-induced comorbid malocclusion
DOI:
https://doi.org/10.14739/2310-1210.2019.6.186484Keywords:
gentic polymorphism, interleukin-4, bronchial asthma, allergic rhinitis, orthodontic pathology, childrenAbstract
Purpose. To determine the frequency of interleukin-4 (IL-4) (C-589T, rs2243250) single nucleotide polymorphism in children with bronchial asthma (BA) and allergic rhinitis (AR) and with isolated or allergic rhinitis-induced comorbid malocclusion.
Materials and methods. Single nucleotide polymorphism of the IL-4 (C-589T, rs2243250) gene was analyzed in 170 children aged between 6 and 17 years, 11 months, 29 days. Group I included 89 children with BA; Group II consisted of 31 children with AR; Group III was composed of 27 children with AR and distal occlusion (DO); Group ІV comprised 23 children with malocclusion. Genotyping was performed using a commercial “SNP-express RT” kit by real-time polymerase chain reaction method (Applied Biosystems, USA) via TagMan®SNP Genotyping Assay on an amplifier CFX96TM Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.,USA). DNA was isolated using a commercial DNA-express kit (“LLC Research and Production Company LITEKH”). The general, recessive and dominant models of inheritance and the odds ratios with a 95 % confidence interval were used for the analysis. The results analysis was conducted using the Statistica 6.0RU licensed software package.
Results. IL-4 (C-589T, rs2243250) gene polymorphism in children with allergy and malocclusion living in Zaporizhzhia was analyzed for the first time. The frequency of C/C – C/T – T/T genotypes registration was 69.66 % – 22.47 % – 7.87 % of cases in children with BA; 58.06 % – 38.71 % – 3.23 % in children with AR; 62.96 % – 29.63 % – 7.40 % in AR with DO. On the contrary, in children with malocclusion, the C/C (34.78 %) and T/T (4.35 %) genotypes were registered less frequently and the C/T genotype (60.87 %) was recorded more often as a genetic feature of the DO phenotype.
Conclusions. The C/C genotype of IL-4 (C-589T, rs2243250) gene was associated with bronchial asthma (OR = 4.31; 95 % CI = 1.63–11.36; P = 0.002) and allergic rhinitis (OR = 4.32 (95% CI = 1.04–7.81; P = 0.04), in comparison with the fact that the C/T + T/T genotype indicated a predisposition to malocclusion development.
References
Besh, L.V., Lasytsia, T.S., & Besh, O.M. (2017). Bronkhialna astma v praktytsi simeinoho likaria: suchasni standarty bazysnoi farmakoterapii [Bronchial asthma in family physician practice: current standards of basic pharmacotherapy]. Allergy in a child, 21/22, 8-10. [in Ukrainian].
Caniatti, M., Marchioro, A., Guilherme, A., & Tsuneto, L. (2014). Association of Cytokines in Individuals Sensitive and Insensitive to Dust Mites in a Brazilian Population. PLOS ONE, 9(9), e107921. doi: 10.1371/journal.pone.0107921
Christiansen, E., Kjaer, H., Eller, E., Bindslev-Jensen, C., Høst, A., Mortz, C., & Halken, S. (2016). The prevalence of atopic diseases and the patterns of sensitization in adolescence. Pediatric Allergy And Immunology, 27(8), 847-853. doi: 10.1111/pai.12650
Jacob, L., Keil, T., & Kostev, K. (2016). Comorbid disorders associated with asthma in children in Germany – National analysis of pediatric primary care data. Pediatric Allergy And Immunology, 27(8), 861-866. doi: 10.1111/pai.12656
Langdon, C., Guilemany, J., Valls, M., Alobid, I., Bartra, J., & Dávila, I. et al. (2016). Allergic rhinitis causes loss of smell in children: The OLFAPEDRIAL study. Pediatric Allergy And Immunology, 27(8), 867-870. doi: 10.1111/pai.12655
Shamsidinov, A. T., Fajzov, K. S., Tanzhiholov, U. H., & Ismoilov F. N. (2016). Sistema rannego vyjavlenija i reabilitacii detej i podrostkov goroda Dushanbe s zubocheljustnymi anomalijami [System for early detection and rehabilitation of children and adolescents with dentoalveolar anomalies in Dushanbe]. Herald of institute of postgraduate education in health sphere, 1. Retrieved from http://www.vestnik-ipovszrt.tj/?p=2315. [in Russian].
Shamov, S. M. (2013). Vzaimosvjaz obshhesomaticheskoj patologi i zubocheljustnyh anomalij u detej i podrostkov Respubliki Dagestan [The relationship between somatic pathology and in children and adolescents in Dagestan]. Kuban Scientific Medical Bulletin, 6(141), 193-195. [in Russian].
Attia, M., & Saber, H. (2014). Role played by T-helper 2 in resetting the cytokine balance in allergic patients. The Egyptian Journal Of Internal Medicine, 26(3), 124-129. doi: 10.4103/1110-7782.145311
Puhlik, B. M. (2017). Problemy nasledstvennosti v vozniknovenii allergicheskih zabolevanij [Heredity problems in the occurrence of allergic diseases]. Klinichna immunolohiia. Alerholohiia. Infektolohiia, 1(98), 29-32. Retrieved from https://kiai.com.ua/ru-issue-article-1389/Problemy-nasledstvennosti-v-vozniknovenii-allergicheskih-zabolevaniy. [in Russian].
Golovatjuk, E. P. (2017). Rol polimorfizmov genov IL-4 i IL-17 v privychnom nevynashivanii beremennosti, nastupivshej v ciklah VRT [Role of gene polymorphism of il-4 and il-17 in recurrent miscarriage, came in art cycles]. Reproductive Endocrinology, 1(33),28-31. [in Russian].
Ensembl. Retrieved from http://www.ensembl.org/index.html.
Ivanova, V. B. (2017). Associacii polimorfnyh genov immunnogo otveta so stepenju tjazhesti i urovnem kontrolja atopicheskoj bronhialnoj astmy (Dis. magister.). [Associations of polymorphic genes of the immune response with the severity and level of control of atopic bronchial asthma. Mast. diss.]. Krasnoyarsk. Retrieved from http://elib.sfu-kras.ru/handle/2311/69113. [in Russian].
Ramphul, K., Lv, J., Hua, L., Liu, Q., Fang, D., & Ji, R. et al. (2014). Single nucleotide polymorphisms predisposing to asthma in children of Mauritian Indian and Chinese Han ethnicity. Brazilian Journal Of Medical And Biological Research, 47(5), 394-397. doi: 10.1590/1414-431x20143751
Gureva, L. L. (2015). Prognozirovanie kontrolja atopicheskoj bronhialnoj astmy v detskom vozraste. (Avtoref. dis... dok. med. nauk). [Prediction of childhood atopic asthma control]. (Extended abstract of candidate’s thesis). Samara. [in Russian].
Smolnikova, M. V., Frejdin, M. B., & Smirnova, S. B. (2017). Geny citokinov kak geneticheskie markery atopicheskoj bronhialnoj astmy s kontroliruemym i nekontroliruemym techeniem [Cytokine genes as genetic markers of controlled and uncontrolled atopic bronchial asthma]. Medical Immunology (Russia), 19(5), 605-614. doi: 10.15789/1563-0625-2017-5-605-614 [in Russian].
Kostina, E. M., Molotilov, B. A., Levashova, O. A., & Osipova, M. V. (2013). Izuchenie polimorfizma genov citokinov IL-4, IL-10, IL-17A i TNFA u bolnyh s infekcionno-zavisimoj bronhialnoj astmoj [The study of polymorphism of cytokine genes IL-4, IL-10, IL-17A and TNFA in patients with infectious dependent bronchial asthma]. Immunopathology, allergology, infectology, 1, 53-58. [in Russian].
Hotko, E. A., & Taganovich, A. D. (2016). Polimorfizm genov receptorov i ih ligandov pri hronicheskoj obstruktivnoj bolezni legkih [Gene polymorphism of receptors and their ligands in chronic obstructive pulmonary disease]. Medical Journal, 3, 36-42. [in Russian].
Akparova, A. Ju., Bersimbaj, R. I., Bekmanov, B. O., Eshhzhanov, T. E., & Abishev, M. T. (2012). Rol genov citokinov IL-4 i TNF-A v razvitii predraspolozhennosti k bronhialnoj astme i hronicheskoj obstruktivnoj bolezni legkih [The role of the cytokine genes IL4 and TNF-a in the development of a predisposition to bronchial asthma and chronic obstructive pulmonary disease]. Molecular and geneticresearchmethods in medicine and biology. Proceedings of the International Scientific and Practical Conference. (pp. 15-22). Karaganda. [in Russian].
Kuzmina, L. P., Hotuleva, A. G., Anohin, N. N., & Anvarul N. A. (2018). Geneticheskij polimorfizm protivovospalitelnyh citokinov v ocenke riska razvitija i prognoza techenija professionalnoj bronholegochnoj patologii [Genetic polymorphism of anti-inflammatory cytokines in assessing developmental risk and prognosis of the course of occupational bronchopulmonary pathology]. Health and the environment. Proceedings of the International Scientific and Practical Conference. (Vol. 1. pp. 157-158). Minsk. [in Russian].
Movahedi, M., Amirzargar, A., Nasiri, R., Hirbod-Mobarakeh, A., Farhadi, E., & Tavakol, M. et al. (2013). Gene polymorphisms of Interleukin-4 in allergic rhinitis and its association with clinical phenotypes. American Journal Of Otolaryngology, 34(6), 676-681. doi: 10.1016/j.amjoto.2013.05.002
Korzycka-Zaborowska, B., Zielińska-Bliźniewska, H., Zaborowski, A., & Olszewski, J. (2015). Association of -590 C/T Il-4 Gene Promoter Polymorphism With Atopy in Polish Patients With Allergic Rhinitis. Allergy Disorders & Therapy, 2(1), 1-3. doi: 10.24966/adt-749x/100004
Li, Z., Yin, L., Wang, H., & Liu, L. (2014). Association between promoter polymorphisms of interleukin-4 gene and allergic rhinitis risk: a meta-analysis. Journal Of Huazhong University Of Science And Technology [Medical Sciences], 34(3), 306-313. doi: 10.1007/s11596-014-1275-3
Micheal, S., Minhas, K., Ishaque, M., Ahmed, F., Ahmed, A. (2013). IL-4 gene polymorphisms and their association with atopic asthma and allergic rhinitis in Pakistani patients. Journal of Investigational Allergology and Clinical Immunology, 23(2),107-111.
Grigorovich, Je. Sh. (2016). Hronicheskij generalizovannyj parodontit: kliniko-morfologicheskie i molekuljarno-geneticheskie osnovy geterogennosti zabolevanija, obosnovanie prognoza i personifikacija terapii (Dis... dokt. med. nauk.). [Chronic generalized periodontitis: clinical, morphological and molecular genetic foundations of disease heterogeneity, substantiation of prognosis and personification of therapy. Dr. med. sci. diss.]. Omsk. [in Russian].
Jahina, Z. H., Shirjak, T. Ju., & Kamaldinova, A. R. (2018). Vlijanie rannej poteri zubov na formirovanie zubocheljustnyh anomalij [Effect of early loss of teeth on formacion of dental anomalies]. Modern problems of science and education, 2. Retrieved from http://www.science-education.ru/ru/article/view?id=27536. [in Russian].
Isamulaeva, A. Z., Spicyna, A. V., Magomedov, Sh. Sh., Shatueva, S. Z., & Isamulaeva, A. I. (2014). Znachimost citokinovoj reguljacii v patogeneze zabolevanij polosti rta [The significance of cytokine regulation in the pathogenesis of oral diseases]. Modern problems of science and education, 6. Retrieved from http://www.science-education.ru/ru/article/view?id=15411. [in Russian].
Bartova, J., Borilova Linhartova, P., Podzimek, S., Janatova, T., Svobodova, K., & Fassmann, A. et al. (2014). The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls. Mediators Of Inflammation, 1-11. doi: 10.1155/2014/185757
Anovazzi, G., Medeiros, M., Pigossi, S., Finoti, L., Mayer, M., & Rossa, C. et al. (2017). Functional Haplotypes in Interleukin 4 Gene Associated with Periodontitis. PLOS ONE, 12(1), e0169870. doi: 10.1371/journal.pone.0169870
Hejgetjan, A. V., Bragin, E. A., Maksjukov, S. Ju., Labushkina, A. V., Alutina Je. L., & Harseeva, G. G. (2015). Immunologicheskie pokazateli u bolnyh s kariesom kontaktnyh poverhnostej bokovyh zubov [The immunologic indicators in patients withcaries of contact surfaces]. Russian Clinical Laboratory Diagnostics, 60(8), 52-54. [in Russian].
Yan, Y., Weng, H., Shen, Z., Wu, L., & Zeng, X. (2014). Association Between Interleukin-4 Gene −590 C/T, −33 C/T, and 70-Base-Pair Polymorphisms and Periodontitis Susceptibility: A Meta-Analysis. Journal Of Periodontology, 85(11), e354-362. doi: 10.1902/jop.2014.140317
JIA, Xaowei, MA, Yan, ZENG, Xiantao, YUAN, Yadi, LIN, Yingmei, & SHAO, Jun et al. (2017). Association between -590C/T polymorphisms of interleukin-4 gene and periodontitis: A meta-analysis. Journal of Prevention and Treatment For Stomatological Diseases. 25(6), 354-359. doi: 10.12016/j.issn.2096-1456.2017.06.003
Emam, A., Shehab, M., Allah, M., Elkoumi, M., Abdelaal, N., & Mosabah, A. et al. (2019). Interleukin-4 -590C/T gene polymorphism in Egyptian children with acute lower respiratory infection: A multicenter study. Pediatric Pulmonology, 54(3), 297-302. doi: 10.1002/ppul.24235
Epifanceva, N. V. (2011). Rol polimorfizma genov IL-4 (S589T), IL-10 (S592A, G1082A, S819T) i FNOα (G308A) v patogeneze kokljusha u detej. Dis …kand. med. nauk [The role of IL-4 (C589T), IL-10 (C592A, G1082A, C819T) and TNFα (G308A) gene polymorphism in the pathogenesis of pertussis in children. Dr. med. sci. diss.]. Chita. [in Russian].
Bataeva, E. P. (2014). Vlijanie polimorfizmov genov Il-4 (S589T) i TNFα (G308A) na soderzhanie citokinov u detej pri pielonefritah [Influence polymorphism of genes IL-4 (С589Т) and TNFα (G308А) on content of cytokine in children with pyelonephritis]. Far East Medical Journal, 1, 74-78. [in Russian].
Usychenko, E. N., Usychenko, E. M., & Bazhora, Ju. I. (2017). Sostojanie immunnogo statusa i polimorfizm genov citokinov IL-10, IL-4, TNF u pacientov s hronicheskim gepatitom S v zavisimosti ot stepeni fibroza [Immune status and gene polymorphism of cytokines IL-10, IL-4, TNF in patients with chronic hepatitis C depending on the degree of fibrosis]. Vestnik Of Vitebsk State Medical University, 16(1), 50-58. doi: 10.22263/2312-4156.2017.1.50 [in Russian].
Shumilov, D. S., Tuguz, A. R., Smolkov, I. V., Tatarkova, E. A., Muzhenja, D. V., & Ashkanova, T. M. et al. (2017). Polimorfizmy genov osnovnyh pro- i protivovospalitelnyh citokinov: IL-1β (T511C, RS16944), TNF-α (G308A, RS1800629), IL-4 (C589T, RS2243250) pri koronarnom ateroskleroze [Gene polymorphisms of the main pro- and anti-inflammatory cytokines: IL-1β (T511C, RS16944), TNF-α (G308A, RS1800629), IL-4 (C589T, RS2243250) in coronary atherosclerosis]. The Bulletin of the Adyghe State University: Internet Scientific Journal, 1(196), 41-46. [in Russian].
Puzyreva, L. V., & Safonov, A. D. (2016). Geneticheskij polimorfizm citokinov: proshloe i budushhee [Cytokines genetic polymorphism: the past and the future]. Russian Journal of Infection and Immunity, 6(2), 103-108. doi: 10.15789/2220-7619-2016-2-103-108 [in Russian].
Anohina, E. N., Tuguz, A. R., Rudenko, K. A., & Muzhenja, D. V. (2014). Associacija polimorfizmov genov IL-4, IL-2 so zlokachestvennymi novoobrazovanijami zhenskih reproduktivnyh organov [Association of IL-4 and IL-2 gene polymorphisms with the malignant growths of female reproductive organs]. The Bulletin of the Adyghe State University: Internet Scientific Journal, 1(133), 51-55. [in Russian].
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