The state of the duodenal villi in rats under epichlorohydrin and drugs influence
DOI:
https://doi.org/10.14739/2310-1210.2019.6.186643Keywords:
duodenum, villi, mucosa, epichlorohydrin, ratsAbstract
Introduction. The modern environment is polluted by xenobiotics which can cause the development of pathological processes. One of xenobiotics is epichlorohydrin (ECH). The impact of this xenobiotic causes changes in the state of various organs, including the organs of the digestive system. However, the effects of ECH on the duodenum have not been investigated.
The aim of the study is to investigate the changes in the state of the duodenal villi in rats and changes in mitotic activity of mucous epithelial cells under ECH influence as well as to evaluate a possibility and effectiveness of Echinacea purpurea (EP) extract and thiotriazoline use for these changes correction.
Material and methods. An experimental study was performed on male white rats. Six experimental groups were created. Six rats from each experimental group were removed from the experiment on the 1st, 7th, 15th, 30th, 60th days after the end of the two-month administration of ECH, EP extract and thiotriazoline. The height of the villi of the duodenal mucosa and changes in mitotic activity of mucous epithelial cells were estimated using a microscope of the MS 100 series of Micros (Austria).
Results. Morphofunctional organization of duodenal mucosa in rats was changed after the long-term introduction of ECH. The height of villi and mitotic activity of mucous epithelial cells were reduced. Over time, the expressiveness of the changes diminished. Introduction of EP extract and thiotriazoline administration to rats which did not receive ECH was accompanied by an increase in the height of villi. In the case of thiotriazoline administration, this effect was longer. The use of EP extract as well as the use of thiotriazoline during ECH inhalations reduced the severity and duration of the decrease in the height of villi and mitotic activity of mucous epithelial cells caused by this chemical. The negative effects of ECH were corrected more effectivelly by thiotriazoline than by EP extract.
Conclusions. Prolonged action of ECH is accompanied by negative changes in the state of duodenal mucosa. The negative effects of ECH were corrected more effectivelly by thiotriazoline than by EP extract.
References
Lee, I., Kim, K., Kim, S., Baek, H., Moon, C., & Kim, S. et al. (2013). Apoptotic cell death in rat epididymis following epichlorohydrin treatment. Human & Experimental Toxicology, 32(6), 640-646. doi: 10.1177/0960327112467042
Langan, L., Arossa, S., Owen, S., & Jha, A. (2018). Assessing the impact of benzo[a]pyrene with the in vitro fish gut model: An integrated approach for eco-genotoxicological studies. Mutation Research/Genetic Toxicology And Environmental Mutagenesis, 826, 53-64. doi: 10.1016/j.mrgentox.2017.12.009
Schiavoni, G., D'Amato, G., & Afferni, C. (2017). The dangerous liaison between pollens and pollution in respiratory allergy. Annals Of Allergy, Asthma & Immunology, 118(3), 269-275. doi: 10.1016/j.anai.2016.12.019
Tong, S., Berry, H., Ebi, K., Bambrick, H., Hu, W., & Green, D. et al. (2016). Climate change, food, water and population health in China. Bulletin Of The World Health Organization, 94(10), 759-765. doi: 10.2471/blt.15.167031
Luo, J., Cheng, T., Kuo, H., & Chang, M. (2004). Decreased lung function associated with occupational exposure to epichlorohydrin and the modification effects of glutathione s-transferase polymorphisms. Journal Of Occupational And Environmental Medicine, 46(3), 280-286. doi: 10.1097/01.jom.0000116807.73005.97
Wan, X., Zhao, Z., Qiu, J., Guo, Y., & Wu, J. (2015). Determination of epichlorohydrin in workplace air by gas chromatograph-electron capture detector. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi, 33(4), 307-309. [in Chinese].
Oláh, A., Szabó-Papp, J., Soeberdt, M., Knie, U., Dähnhardt-Pfeiffer, S., Abels, C., & Bíró, T. (2017). Echinacea purpurea -derived alkylamides exhibit potent anti-inflammatory effects and alleviate clinical symptoms of atopic eczema. Journal Of Dermatological Science, 88(1), 67-77. doi: 10.1016/j.jdermsci.2017.05.015
Jiang, L., Li, W., Wang, Y., Zhang, X., Yu, D., & Yin, Y. et al. (2014). Effects of cichoric acid extract from Echinacea purpurea on collagen-induced arthritis in rats. The American Journal Of Chinese Medicine, 42(03), 679-692. doi: 10.1142/s0192415x1450044x
Rezaie, A., Fazlara, A., Haghi Karamolah, M., Shahriari, A., Najaf Zadeh, H., & Pashmforosh, M. (2013). Effects of Echinacea purpurea on hepatic and renal toxicity induced by diethylnitrosamine in rats. Jundishapur journal of natural pharmaceutical products, 8(2), 60-64.
Krishnaraj, J., Kowshik, J., Sebastian, R., Raghavan, S., & Nagini, S. (2017). Exposure to welding fumes activates DNA damage response and redox-sensitive transcription factor signalling in Sprague-Dawley rats. Toxicology Letters, 274, 8-19. doi: 10.1016/j.toxlet.2017.04.001
Waidyanatha, S., Gaudette, N., Hong, Y., & Fennell, T. (2014). Formation of epichlorohydrin, a known rodent carcinogen, following oral administration of 1,3-dichloro-2-propanol in rats. Chemical Research In Toxicology, 27(10), 1787-1795. doi: 10.1021/tx500239q
Tanabe, T., Yamaguchi, N., Okuda, M., Ishimaru, Y., & Takahashi, H. (2015). Immune System Reaction against Environmental Pollutants. Nippon Eiseigaku Zasshi (Japanese Journal Of Hygiene), 70(2), 115-119. doi: 10.1265/jjh.70.115 [in Japanese].
Ridano, M., Racca, A., Flores-Martin, J., Fretes, R., Bandeira, C., & Reyna, L. et al. (2017). Impact of chlorpyrifos on human villous trophoblasts and chorionic villi. Toxicology And Applied Pharmacology, 329, 26-39. doi: 10.1016/j.taap.2017.05.026
Shin, I., Lim, J., Kim, S., Kim, K., Park, N., & Bae, C. et al. (2010). Induction of Oxidative Stress in the Epididymis of Rats After Subchronic Exposure to Epichlorohydrin. Bulletin Of Environmental Contamination And Toxicology, 84(6), 667-671. doi: 10.1007/s00128-010-0018-9
Nikolaeva, O., & Smirnov, A. (2017). Status of euchromatin in mucous cells nuclei of pyloric glands after inhalation of epichlorohydrin and correction of emerging changes. Inter Collegas, 4(1), 29-32. doi: 10.35339/ic.4.1.29-32
Shin, I., Park, N., Lee, J., Kim, K., Moon, C., & Kim, S. et al. (2010). One-generation reproductive toxicity study of epichlorohydrin in Sprague-Dawley rats. Drug And Chemical Toxicology, 33(3), 291-301. doi: 10.3109/01480541003734030
Shanh, F., Rahimnejad, S., Bahrami, A., & Farhadian, M. (2017). Risk Assessment of Workers' Exposure to Volatile Organic Compounds in the Air of a Petrochemical Complex in Iran. Indian Journal Of Occupational And Environmental Medicine, 21(3), 121-127. doi: 10.4103/ijoem.ijoem_86_17
Rutkowska, A., & Diamanti-Kandarakis, E. (2016). Polycystic ovary syndrome and environmental toxins. Fertility And Sterility, 106(4), 948-958. doi: 10.1016/j.fertnstert.2016.08.031
Saeedi Saravi, S., & Dehpour, A. (2016). Potential role of organochlorine pesticides in the pathogenesis of neurodevelopmental, neurodegenerative, and neurobehavioral disorders: A review. Life Sciences, 145, 255-264. doi: 10.1016/j.lfs.2015.11.006
Gan, C., Liu, L., Du, Y., Wang, L., Gao, M., & Wu, L. et al. (2016). Simultaneous determination and pharmacokinetic study of four phenol compounds in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry after oral administration of Echinacea purpurea extract. Journal Of Separation Science, 39(9), 1628-1637. doi: 10.1002/jssc.201600051
Aarland, R., Bañuelos-Hernández, A., Fragoso-Serrano, M., Sierra-Palacios, E., Díaz de León-Sánchez, F., & Pérez-Flores, L. et al. (2017). Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts. Pharmaceutical Biology, 55(1), 649-656. doi: 10.1080/13880209.2016.1265989
Taboureau, O., & Audouze K. (2017). Human environmental disease network: A computational model to assess toxicology of contaminants. ALTEX, 34(2), 289-300. doi: 10.14573/altex.1607201
Darwish, W., Ikenaka, Y., Nakayama, S., Mizukawa, H., Thompson, L., & Ishizuka, M. (2017). β-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line. Environmental Science And Pollution Research, 25(7), 6320-6328. doi: 10.1007/s11356-017-0977-z
Kuvenjova, M. L., Luzin, V. I., Morozov, V. N., & Morozova, E. N. (2016). Vlijanie jekstrakta jehinacei purpurnoj na strukturu slizistoj obolochki i kolichestvo kletok sobstvennyh zhelez slizistoj obolochki fundalnogo otdela zheludka krys [Influence of echinacea purple extract on the structure of the mucosa and number of proper gastric glands cells of fundic mucosa of rats’ stomach]. Kursk Scientific and Practical Bulletin "Man and His Health", 2, 66-68. doi: 10.21626/vestnik/2016-2/12 [in Russian].
Cydendambaev, P. B., Baldanova, I. R., Erentueva, A. Ju., & Abidueva L. R. (2018). Vlijanie jekstraktov lekarstvennyh rastenij bajkalskogo regiona na pokazateli immuniteta i gemostaza pri jeksperimentalnom immunodeficite [Extracts of medicinal plants in baikal region:their effect on the immunity and hemostasis indicators in case of experimental immunodeficiency]. Bulletin of the Buryat State University. Medicine and pharmacy, 1, 9-16. [in Russian].
Voloshina, I. S. (2017). Osobennosti korrekcii tiotriazolinom morfologicheskih izmenenij prostaty i semennyh puzyrkov krys, vyzvannyh vozdejstviem parov jepihlorgidrina [Use of tiotriazoline for correction of morphological changes in rat’s prostate and seminal vesicles caused by exposure to epichlorhydrin vapor]. Ulyanovsk Medico-Biological Journal, 4, 133-139. doi: 10.23648/UMBJ.2017.28.8751 [in Russian].
Voloshyna, I. S. (2014). Histolohichna budova vnutrishnikh orhaniv reproduktyvnoi systemy statevozrilykh shchuriv samtsiv pislia tryvaloho vplyvu na orhanizm epikhlorhidrynu [Changes in the histological structure of the internal organs of the reproductive system of immature rats after inhalation exposure to toluene]. Bulletin of problems biology and medicine, 1(106), 230-236. [in Ukrainian].
Vysockij, I. Ju., & Vysockaja, E. I. (2009). Vlijanie metabolitotropnyh preparatov na nekotorye pokazateli jenergeticheskogo obmena v uslovijah ostrogo toksicheskogo porazhenija pecheni letuchimi komponentami jepoksidnoj smoly JeD-20 [The influence of metabolytotropic medicines on some indices of energetic exchange in the conditions of acute toxic liver damage by flowing components of epoxyde tar ED-20]. Journal of SSU. Series MEDICINE, 1, 15-21. [in Russian].
Vysockij, I. Ju. (2005). Farmakologicheskaja korrekcija narushenija prostaciklin-tromboksanovogo ravnovesija pri ostrom toksicheskom porazhenii pecheni letuchimi komponentami dianovoj jepoksidnoj smoly [Pharmacological correction of prostacyclin-thromboxane imbalance in acute toxic liver damage with volatile components of diane epoxy]. Modern problems of toxicology, 1, 51-56. [in Russian].
Gavrilov, V. A., & Luzin, V. I. (2014). Rost i formoobrazovanie nizhnej cheljusti u belyh krys razlichnogo vozrasta posle 60-dnevnogo vozdejstvija parov jepihlorgidrina [Growth and formation of mandible in rats of different ages after 60-day inhalation of epichlorohydrin]. World of medicine and biology, 4(47), 93-97. [in Russian].
Luzin, V. I., Fastova, O. N., Morozov, V. N., & Morozova, E. N. (2016). Gistomorfometricheskie parametry distalnyh izvityh kanalcev pochek krys razlichnyh vozrastnyh grupp na fone ingaljacionnogo vozdejstvija toluola s primeneniem korrektorov [Histomorphometric parameters of renal distal convoluted tubules in rats of different age periods on the background of toluene inhalation with the use of correctors]. Scientific bulletins of Belgorod State University. Series: Medicine. Pharmacia, 5(226), 99-103. [in Russian].
Demkovych, A. Ye. (2014).Vplyv tiotryazolinu na aktyvnist tsytokinohenezu u krovi v protsesi rozvytku eksperymentalnoho postekstraktsiinoho alveolitu [Effect of tiotriazoline on development process of cytokinegenesis activity in experimental postextraction alveolitis]. Bulletin of problems biology and medicine, 1(4), 355-359. [in Ukrainian].
Zaharov, V. B., Smirnov, S. N., Mamontov, S. G., & Zaharova E. T. (2005). Dinamika kletochnoj proliferacii v pecheni krys v rannem postnatalnom ontogeneze i rol jepidermalnogo faktora rosta v organizacii proliferativnogo rezhima [Time course of cell proliferation in rat liver in the early postnatal ontogeny and role of epidermal growth factor in organization of proliferative regimen]. Bulletin of Experimental Biology and Medicine, 139(5), 585-588. [in Russian].
Shajahmetov, S. F., Bodienkova, G. M., Meshhakova, N. M., & Kurchevenko S. I. (0122). Izmenenija immunoreaktivnosti u rabotnikov himicheskih proizvodstv v zavisimosti ot dozovoj nagruzki toksikantami [Alterations of immunoreactivity in cnemical production workers in dependence on dose exposure to toxicants]. Hygiene and sanitation, 91(4), 40-43. [in Russian].
Kasimov, Sh. A., Dzhalilov, A. T., & Turaev, H. H. (2017). Sintez sorbenta na osnove di-(2-aminojetila)-ditiofosfata kalija i jepihlorgidrina [The sorbent synthesis based on di-(2-aminoethyl)-dithiophosphate of potassium and epichlorohydrin]. Universum: chemistry and biology, 9(39),16-21. [in Russian].
Luzin, V. I., Skorobogatov, A. N., & Morozov, V. N. (2013). Osobennosti rosta kostej skeleta belyh krys razlichnogo vozrasta posle ingaljacionnoj zatravki toluolom [Features of bone growth in white rats of different ages after inhalation of toluene vapors]. Taurian Medical and Biology Journal, 16(1), 135-138. [in Russian].
Belenichev, I. F., Vizir, V. A., Mamchur, V. I., & Kurjata, A. V. (2019). Mesto tiotriazolina v galeree sovremennyh metabolitotropnyh lekarstvennyh sredstv [Place of tiotriazoline in the gallery of modern metabolitotropic medicines]. Zaporozhye medical journal, 21(1), 118-128 doi: 10.14739/2310-1210.2019.1.155856 [in Russian].
Mustafaeva, R. Je., Movlaev, I. G., & Ibragimova, S. M. (2015). Poluchenie i issledovanie rezinovoj smesi na osnove izoprenovogo i modificirovannogo oligojefirmetakrilatom jepihlorgidrina butadien-stirolnogo kauchukov [Obtaining and investigation of rubber mixtures on the basis of isoprene and modified oligoethermetacrylate epichlorhydrine by styrene butadiene rubber]. Industrial production and use of elastomers, 1, 37-39. [in Russian].
Gromova, O. A., Torshin, I. Ju., Pronin, A. V., & Volkov, A. Ju. (2016). Sinergichnye nejrotroficheskie jeffekty piracetama i tiotriazolina [Synergistic neurotrophic effects of piracetam and thiotriazoline]. Neurology, Neuropsychiatry, Psychosomatics, 8(1), 86-89. doi: 10.14412/2074-2711-2016-1-86-89 [in Russian].
Skorobogatov, A. N., & Pastuhova, V. A. (2017).Vozrastnye osobennosti rosta i formoobrazovanija kostej skeleta posle 60-dnevnogo vozdejstvija na organizm parov jepihlorgidrina [Age peculiarities of growth and formation of skeletal bones after influence of the 60-day exposure to epichlorhydrin vapor on organism]. Bulletin of problems biology and medicine, 4(2), 355-359. [in Russian].
Skorobogatov, A. N., & Gavrilov, V. A. (2014). Prochnostnye harakteristiki nizhnih cheljustej i plechevyh kostej u nepolovozrelyh belyh krys posle dlitel'nogo vlijanija parov toluola [Strength features of rat mandible and humerus after long-term influence of toluene vapors]. Bulletin of problems biology and medicine, 2(2), 138-141. [in Russian].
Lukianchuk, V. D., Seifullina, I. Y., Rysukhina, N. V., Martsynko, O. E., & Tkachenko V. M. (2007). Skryninh i porivnialna otsinka efektyvnosti zasobiv detoksykatsii sered koordynatsiinykh spoluk hermaniiu z biolihandamy pry syndromi tryvaloho rozchavliuvannia [Screening and comparative efficiency analysis of detoxicative remedies among organic germanium compounds in crush syndrome]. Odessa medical journal, 1(99). 15-19. [in Ukrainian].
Smirnov, A. S., Smirnov, S. N., & Mirzebasov, M. A. (2016). Izmenenija sostojanija sekretornyh granul poverhnostnyh jepiteliocitov slizistoj obolochki piloricheskogo otdela zheludka krys pri dejstvii jepihlorgidrina [Changes the state of secretory granules of the superficial epitheliocytes of the pyloric mucous membrane in the stomach of rats under the action of epichlorohydrin]. Zaporozhye medical journal, 5(98), 89-92. doi: 10.14739/2310-1210.2016.5.82693 [in Russian].
Smirnov, S. N. (2010).Vzaimodejstvie desimpatizacii i tireoidnyh gormonov v organizacii ritmov DNK-sinteticheskoj i mitoticheskoj aktivnosti jepiteliocitov jazyka nepolovozrelyh krys [Co-operation of sympathectomy and thyroxin in the regulation of ton- gue’s epithelial cells division of immature rats]. Ukrainian morphological Almanac, 8(2), 200-202. [in Russian].
Bodienkova, G. M., Boklazhenko, E. V., Kurchevenko, S. I., & Alekseev, R. Ju. (2014). Sostojanie i dinamika narushenij immunoreaktivnosti u rabotnikov himicheskih proizvodstv [Disorder state and dynamics of immune responsiveness in employees of chemical productions]. Bulletin of the ESRC SB RAMS, 1(95), 9-13. [in Russian].
Sur, S. V., Solobjukova, N. A., & Makarov, A. A. (2015). Farmakojekonomicheskij analiz lechenija pacientov s ostrym koronarnym sindromom bez podema segmenta ST v Ukraine [Pharmacoeconomic analysis of treating patients with acute coronary syndrome without ST-segment elevation in Ukraine]. Emergency medicine, 6(69), 30-35. [in Russian].
Koveshnikov, V. G., Fomina, K. A., Luzin, V. I., Voloshin,V. N., Voloshina, I. S., & Shutov, E. Ju. et al. (2013). Jekologicheskaja morfologija organov jendokrinnoj, immunnoj i kostnoj sistem v uslovijah hronicheskogo ingaljacionnogo vlijanija letuchih komponentov jepoksidnyh smol [The ecological morphology of the organs of endocrine, immune and bone systems under the of chronic inhalation influence of volatile components of epoxy resins]. Astrakhan medical journal, 8(1), 128-130. [in Russian].
Koveshnikov, V. G., Fomina K. A., Voloshin, V. N., Rykova, Ju. A., Belik, I. A., & Gladkova, A. N. et al. (2010). Jeksperimentalnoe issledovanie vlijanija tiotriazolina na organy i sistemy organizma [Experimental study of the influence of thiotriazoline on organs and systems of the organism]. Zaporozhye medical journal, 12(5), 174-176. [in Russian].
Shovkun, L. A., Kampos, E. D., Konstantinova, A. V., Franchuk, I. M., & Volodko, N. A. (2016). Jeffektivnost patogeneticheskogo lechenija u bolnyh tuberkulezom legkih s jekssudativnym tipom vospalitelnoj tkanevoj reakcii [Efficiency pathogenetic treatment of patients with pulmonary tuberculosis with exudative type inflammatory tissue reaction]. Medical Herald of the South of Russia, 1, 87-91. [in Russian].
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)