Diclofenac sodium effects on spontaneous behavioral activity of rats with osteoarthritis and concomitant hypothyroidism
DOI:
https://doi.org/10.14739/2310-1210.2020.1.194643Keywords:
osteoarthritis, hypothyroidism, diclofenac sodiumAbstract
The aim of the study – to investigate the effect of diclofenac sodium on the spontaneous behavioral activity of rats in conditions of osteoarthritis with concomitant hypothyroidism.
Materials and methods. The study was conducted on 80 white outbred rats of both sexes, weighing 230-250 g, which were kept in standard vivarium conditions of the SI "DMA Ministry of Health of Ukraine". Experimental osteoarthritis was modeled by intraarticular injection of a solution of monoiodoacetic acid, and experimental hypothyroidism was modeled by enteral administration of a 0.02 % solution of carbimazole. After the formation of experimental models on the 42 day of the experiment, animals were selectively divided into 4 study groups of 20 rats in each. L-thyroxine at a dose of 1.5 mg/kg (intragastrically) and diclofenac sodium at a dose of 10 mg/kg (intragastrically) were used in the study. The drugs were administrated daily at the peak of increasing pathological changes from the 42 day of the experiment within 5 days. Changes in the motor and exploratory activity of animals were studied in the open field test.
Results. An analysis of the results obtained revealed inhibition of spontaneous behavioral activity and emotional lability of animals on day 42 of the experiment in all groups. In L-thyroxine administration, the drug ability to influence positively the formed pathological models was found by the open field test, but there was no complete recovery. When prescribing diclofenac sodium together with L-thyroxine, a more effective restoration of motor and exploratory activity was found compared with the prescription of L-thyroxine alone.
Conclusions. Basic replacement therapy with L-thyroxine contributes to the motor and exploratory activity restoration in conditions of osteoarthritis with concomitant hypothyroidism in rats, but without complete recovery. Diclofenac sodium administration together with L-thyroxine contributes to positive changes in the spontaneous behavioral activity in rats with osteoarthritis and concomitant hypothyroidism and has a more significant effect on the open field test indices than L-thyroxine monotherapy.
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