Diclofenac sodium effects on spontaneous behavioral activity of rats with osteoarthritis and concomitant hypothyroidism
Keywords:osteoarthritis, hypothyroidism, diclofenac sodium
The aim of the study – to investigate the effect of diclofenac sodium on the spontaneous behavioral activity of rats in conditions of osteoarthritis with concomitant hypothyroidism.
Materials and methods. The study was conducted on 80 white outbred rats of both sexes, weighing 230-250 g, which were kept in standard vivarium conditions of the SI "DMA Ministry of Health of Ukraine". Experimental osteoarthritis was modeled by intraarticular injection of a solution of monoiodoacetic acid, and experimental hypothyroidism was modeled by enteral administration of a 0.02 % solution of carbimazole. After the formation of experimental models on the 42 day of the experiment, animals were selectively divided into 4 study groups of 20 rats in each. L-thyroxine at a dose of 1.5 mg/kg (intragastrically) and diclofenac sodium at a dose of 10 mg/kg (intragastrically) were used in the study. The drugs were administrated daily at the peak of increasing pathological changes from the 42 day of the experiment within 5 days. Changes in the motor and exploratory activity of animals were studied in the open field test.
Results. An analysis of the results obtained revealed inhibition of spontaneous behavioral activity and emotional lability of animals on day 42 of the experiment in all groups. In L-thyroxine administration, the drug ability to influence positively the formed pathological models was found by the open field test, but there was no complete recovery. When prescribing diclofenac sodium together with L-thyroxine, a more effective restoration of motor and exploratory activity was found compared with the prescription of L-thyroxine alone.
Conclusions. Basic replacement therapy with L-thyroxine contributes to the motor and exploratory activity restoration in conditions of osteoarthritis with concomitant hypothyroidism in rats, but without complete recovery. Diclofenac sodium administration together with L-thyroxine contributes to positive changes in the spontaneous behavioral activity in rats with osteoarthritis and concomitant hypothyroidism and has a more significant effect on the open field test indices than L-thyroxine monotherapy.
Argumedo, G., Sanz, C., & Olguín, H. (2012). Experimental Models of Developmental Hypothyroidism. Hormone and Metabolic Research, 44(2), 79 85. https://doi.org/10.1055/s-0031-1297941
El-bakry, A., El-Gareib, A., & Ahmed, R. (2010). Comparative study of the effects of experimentally induced hypothyroidism and hyperthyroidism in some brain regions in albino rats. International Journal Of Developmental Neuroscience, 28(5), 371 389. https://doi.org/10.1016/j.ijdevneu.2010.04.003
dos Reis-Lunardelli, E., Castro, C., Bavaresco, C., Coitinho, A., da Trindade, L., Perrenoud, M., Roesler, R., Sarkis, J., de Souza Wyse, A., & Izquierdo, I. (2007). Effects of Thyroid Hormones on Memory and on Na+, K+-ATPase Activity in Rat Brain. Current Neurovascular Research, 4(3), 184 193. https://doi.org/10.2174/156720207781387204
Gryb, V. A., & Gerasymchuk, M. R. (2017). Otsinka kohnityvnykh funktsii u shchuriv pry eksperymentalnomu hipotyreozi [Cognitive functions assessment in rats with experimental hypothyroidism]. Actual problems of transport medicine, (2), 110 113. [in Ukrainian].
Demchenko, O. M. (2014). Kohnityvna aktyvnist shchuriv za umov dysfunktsii shchytopodibnoi zalozy [Cognitive Activity of Rats under Thyroid Dysfunction]. Bulletin of problems biology and medicine, 3(2), 127 132. [in Ukrainian].
Demchenko, E. M. (2014). Psykho-emotsiinyi status shchuriv za umov dysfunktsii shchytovydnoi zalozy [Psycho-emotional state of rats under thyroid dysfunction]. Medical perspectives, 19(1), 10 15. [in Ukrainian].
Ge, J., Peng, L., Hu, C., & Wu, T. (2012). Impaired Learning and Memory Performance in a Subclinical Hypothyroidism Rat Model Induced by Hemi-Thyroid Electrocauterisation. Journal Of Neuroendocrinology, 24(6), 953 961. https://doi.org/10.1111/j.1365-2826.2012.02297.x
dos Reis-Lunardelli, E., Ramirez, M., Castro, C., Coitinho, A., Bavaresco, C., da Trindade, L., Perrenoud, M., Wyse, A., Sarkis, J., & Izquierdo, I. (2007). Effects of an Acute Treatment with L-Thyroxine on Memory, Habituation, Danger Avoidance, and on Na+, K+-ATPase activity in Rat Brain. Current Neurovascular Research, 4(4), 259 267. https://doi.org/10.2174/156720207782446360
Guingamp, C., Gegout-Pottie, P., Philippe, L., Terlain, B., Netter, P., & Gillet, P. (1997). Mono-iodoacetate-induced experimental osteoarthritis. A dose-response study of loss of mobility, morphology, and biochemistry. Arthritis & Rheumatism, 40(9), 1670 1679. https://doi.org/10.1002/art.1780400917
Mironov, A. N. (Ed). (2012). Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv [Guidelines for conducting preclinical studies of drugs]. Pt. 2. Moscow. [in Russian].
Gorodetskaya, I. V., & Gusakova, E. A. (2014). Vliyanie iodsoderzhashchikh tireoidnykh gormonov na gistostrukturu pecheni krys pri stresse [Effect of iodine-containing thyroid hormones on the histostructure of rat liver under the stress]. Tsitologiya, 56(3), 225 233. [in Russian].
Nefodov, O. O. (2017). Farmakolohichnyi analiz neiroprotektsii za umov eksperymentalnoho alerhichnoho entsefalomiielitu (Dis… dokt. med. nauk). [Pharmacological analysis of neuroprotection in conditions of experimental allergic encephalomyelitis. Dr. med. sci. diss.]. Kiev. [in Ukrainian].
Podpletnyaya, E. A. (2008). Analiz farmakologicheskoi aktivnosti i bezopasnosti nesteroidnykh protivovospalitel'nykh sredstv i eksperimental'noe obosnovanie optimizatsii boleutolyayushchei i protivovospalitel'noi terapii (Dis… dokt. farm. nauk). [The analysis of pharmacological activity and safety of nonsteroidal anti-inflammatory drugs and experimental ground of anti-inflammatory and analgesic therapy improvement. Dr. farm. sci. diss.]. Kharkiv. [in Russian].
Williams, G. R. (2013). Thyroid hormone actions in cartilage and bone. European thyroid journal, 2(1), 3 13. https://doi.org/10.1159/000345548
Voloshina, L. A., Voloshin, A. I., & Pashkovska, N. V. (2014). Osoblyvosti kompleksnoho likuvannia khvorykh na osteoartroz na tli subklinichnoho hipotireozu [Peculiarities of the complex treatment of patients with osteoarthritis combined with subclinical hypothyroidism]. Aktualni pytannia zberezhennia zdorov’ia liudyny: Proceedings of the International Interdisciplinary Scientific and Practical Conference. (pp. 48 51). Uzhhorod. [in Ukrainian].
Nastanovy dlia pervynnoi medychnoi dopomohy Duodecim. Nastanova 00396. Osteoartroz [Guidelines in primary care Duodecim. Guidance 00396. Osteoarthrosis]. Ministry of Health of Ukraine. https://guidelines.moz.gov.ua/documents/3259. [in Ukrainian].
Nosivets, D. S. (2019). Vliyanie kombinatsii nesteroidnykh protivovospalitel'nykh sredstv na techenie osteoartroza pri soputstvuyushchem gipotireoze [Effect of the combination of nonsteroidal anti-infl ammatory drug on the course of osteoarthritis with concomitant hypothyroidism]. Problems of Endocrine Pathology, (2), 40 45. https://doi.org/10.21856/j-PEP.2019.2.06 [in Russian].
How to Cite
LicenseAuthors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)