Myeloprotective activity of granulocyte-colony stimulating factor drugs and enterosorption in rats grafted with Guerin carcinoma
Keywords:melphalan, granulocyte colony stimulating factor, enterosorption, rats, Guerin carcinoma
The aim of this work is the study of granulocyte-colony stimulating factor (G-CSF) (officinal drug filgrastim and analogue, synthesized by IEPOR, r-GCSF) and enterosorption impact on the bone marrow of Guerin carcinoma-grafted rats with melphalan administration.
Materials and methods. The study was performed in rats grafted with Guerin carcinoma. On the 10th day after Guerin carcinoma cells implantation, melphalan was injected at a dose of 5.5 mg/kg. Drugs of G-CSF and granular carbon oral adsorbent С2 (bulk density of 0.18 g/cm3, granules 0.15–0.25 mm in diameter, total specific surface by BET of 2162 m2/g) were used for correction. Rats were sacrificed on the 17th day after Guerin carcinoma grafting, the indices of peripheral blood and myelograms were examined.
Results. It was found that single injection of alkylating cytostatic agent caused a profound destruction (hypoplasia and aplasia) of bone marrow in rats as well as a drop in leukocytes (by 95.7 %) and granulocytes (by 73.9 %) in peripheral blood. Officinal drug of G-CSF – filgrastim and enterosorbent C2 with bulk density of 0.18 g/cm3, used alone for the pathological condition correction, slightly improved indices (leukocyte count increased by 2.3 and 1.5 times, respectively), but there was only a tendency to improve the granulocyte count. Bone-marrow cellularity increased and there were some differentiating cells. The use of these drugs in combination was the most efficient compared to their separate administration. The granulocyte count in peripheral blood was almost normalized in the groups of rats received the combination of C2 enterosorbent with G-CSF (L-PAM + filgrastim + С2) and (L-PAM + r-GCSF + С2) as it was 3.5 and 3.0 times increased, respectively. The same tendency was observed in the bone marrow.
Conclusions. The results obtained indicate benefits from further study and implementation into clinics the combination of hemostimulating cytokines and enterosorption.
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