Hydroxycinnamic acids in the raw material of hybrid bearded iris

Authors

  • A. V. Krechun National University of Pharmacy, Kharkiv, Ukraine,
  • O. O. Mykhailenko National University of Pharmacy, Kharkiv, Ukraine,
  • V. M. Kovalov National University of Pharmacy, Kharkiv, Ukraine,
  • Yu. V. Buidin M. M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine, Kyiv,
  • T. P. Osolodchenko State Institution “I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine”, Kharkiv,

DOI:

https://doi.org/10.14739/2310-1210.2020.2.200632

Keywords:

iris, hydroxycinnamic acids, antimicrobial activity, chromatography

Abstract

 

Representatives of Iris genus (Iridaceae) are widespread in almost all continents. According to the literature data, Iris species accumulate secondary metabolites such as flavonoids, isoflavonoids, xanthones, coumarins, hydroxycinnamic acids, saponins, tannins, vitamins, organic acids.

The aim of this work was the establishment of the qualitative and quantitative composition of hydroxycinnamic acids in the raw materials of iris hybrid varieties, as well as to establish the antimicrobial activity of the dry extracts and isolation of individual compounds.

Materials and methods. The objects of study were the leaves and rhizomes of the standard dwarf bearded iris (SDB). The substances were isolated by column chromatography, nature was established by physical and physicochemical methods of analysis (UV-, IR spectroscopy, TLC). Antibacterial activity was determined by the agar well diffusion method in vitro.

Results. Hydroxycinnamic acids were identified in the iris raw materials by paper chromatography. The spectrophotometric method established of the hydroxycinnamic acid content in the recalculation on chlorogenic acid – was from 0.79 % to 2.83 %. The dry extracts of the leaves and rhizomes of I. hybrida “Little Dream” at a 1 % concentration have shown moderate inhibitory activity for Gram-positive bacteria and fungi. For the first time from the rhizomes of Iris hybrida “Indian Pow Waw” were isolated caffeic, chlorogenic, neochlorogenic, ferulic acids by the column chromatography and their structures were determined by spectral methods.

Conclusions. The content of hydroxycinnamic acids in the samples varies from 0.79 % to 2.83 %. The antimicrobial activity of the dry extracts of I. hybrida “Little Dream” leaves and rhizomes was established. For the first time from the rhizomes of Iris hybrida “Indian Pow Waw” were isolated 4 derivatives of hydroxycinnamic acid. The results of the study show the prospects for the use of cultivated varieties of irises as a source of hydroxycinnamic acids.

References

Wilson, C. A. (2011). Subgeneric classification inIrisre-examined using chloroplast sequence data. TAXON, 60(1), 27-35. https://doi.org/10.1002/tax.601004

Goldblatt, P., & Manning, J. C. (2008). The Iris family : natural history et classification. Timber Press.

Mykchailenko, O. O., & Kovalyov, M. V. (2016). Phenolic compounds of the genus Iris plants (Iridaceae). Česká a slovenská farmacie, 65(2), 70-77. https://www.researchgate.net/profile/Mikhailenko_Olga/publication/304667804_Phenolic_compounds_of_the_genus_Iris_plants_Iridaceae/links/5922f4bca6fdcc4443f7756d/Phenolic-compounds-of-the-genus-Iris-plants-Iridaceae.pdf.

Tikhomirova, L. I., Bazarnova, N. G., Mikushina, I. V., & Dolganova, Z. V. (2015). Farmakologo-biokhimicheskoe obosnovanie prakticheskogo ispol'zovaniya nekotorykh predstavitelei roda Iris L.(obzor) [Farmacol-biochemical study of practical use of some members of the genus Iris L. (overview)]. Khimiya rastitel'nogo syr'ya, (3), 25-34. https://doi.org/10.14258/jcprm.201503837 [in Russian].

Krechun, A. V., Mykhailenko, O. O., & Kovalyov, V. M. (2016). Organic and hydroxycinnamic acids of Iris hungarica. In R. T. Tulyaganov, S. N. Aminov, & N. T. Farmanova (Eds.). Aktual'nye voprosy obrazovaniya, nauki i proizvodstva v farmatsii: Materialy respublikanskoi nauchno-prakticheskoi konferentsii (s mezhdunarodnym uchastiem) (pp. 6-7). Tashkent.

Cai, Y., Luo, Q., Sun, M., & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157-2184. https://doi.org/10.1016/j.lfs.2003.09.047

Tsimogiannis, D., & Oreopoulou, V. (2019). Classification of Phenolic Compounds in Plants. In R. R. Watson (Ed.). Polyphenols in Plants: Isolation, Purification and Extract Preparation (2nd ed., pp. 263-284). Academic Press. https://doi.org/10.1016/B978-0-12-813768-0.00026-8

dos Santos, M. D., Almeida, M. C., Lopes, N. P., & de Souza, G. E. P. (2006). Evaluation of the Anti-inflammatory, Analgesic and Antipyretic Activities of the Natural Polyphenol Chlorogenic Acid. Biological & Pharmaceutical Bulletin, 29(11), 2236-2240. https://doi.org/10.1248/bpb.29.2236

Matkowski, A., Jamiolkowska-Kozlowska, W., & Nawrot, I. (2013). Chinese Medicinal Herbs as Source of Antioxidant Compounds – Where Tradition Meets the Future. Current Medicinal Chemistry, 20(8), 984-1004. https://doi.org/10.2174/092986713805288888

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2008, February 1). Derzhavna Farmakopeia Ukrainy. Dopovnennia 2 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl. 2). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

McFarland, J. (1907). The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. The Journal of the American Medical Association, XLIX(14), 1176-1178. https://doi.org/10.1001/jama.1907.25320140022001f

State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2004, April 1). Derzhavna Farmakopeia Ukrainy. Dopovnennia 1 [The State Pharmacopoeia of Ukraine] (1st ed., Suppl. 1). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].

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How to Cite

1.
Krechun AV, Mykhailenko OO, Kovalov VM, Buidin YV, Osolodchenko TP. Hydroxycinnamic acids in the raw material of hybrid bearded iris. Zaporozhye medical journal [Internet]. 2020Apr.13 [cited 2024Apr.19];22(2). Available from: http://zmj.zsmu.edu.ua/article/view/200632

Issue

Vol. 22 No. 2 (2020)

Section

Original research

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