Antimicrobial activity of structural-metabolic complexes of L. rhamnosus GG and S. boulardii against S. aureus AТСС 25923, E. coli ATCC 25922, Р. аеruginosa ATCC 27853
DOI:
https://doi.org/10.14739/2310-1210.2020.4.208396Keywords:
metabolites, structural components, antimicrobial properties, saccharomycetes, lactobacteria, reference strainsAbstract
The aim of the work – to determine the sensitivity of reference strains to structural-metabolic complexes of both Lactobacillus rhamnosus GG alone and in combination with Saccharomyces boulardii to justify the possibility of developing antimicrobial drugs with polyfunctional activity.
Materials and methods. Proprietary structural-metabolic complexes of lactobacteria and lactobacteria with saccharomycetes were obtained without the use of culture media. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined by the serial dilution micromethod in a culture medium in a 96-well plate. To estimate the MIC, the optical density of the samples was spectrophotometrically measured using a Lisa ScanTM EM analyzer (Erba Mannheim, Czech Republic), and broth was plated on a solid culture medium for the MBC estimation. The concentrations of the test substances ranged from 1.10 to 0.02 mg/ml by the total protein. The test cultures were reference strains of S. aureus ATCC 25923, E. coli ATCC 25922, P. aeroginosa ATCC 27853.
Results. The antimicrobial effect was found to be in direct proportion to the exposure time, concentration and activity of structural-metabolic complexes of lactobacteria and lactobacteria with saccharomycetes. The MBC of lactobacteria filtrates for S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853 was 0.27 mg/ml by protein, and lactobacteria and saccharomycetes – 0.21 mg/ml by protein. The structural-metabolitic complex of Lactobacillus with a concentration of 0.14 mg/ml by protein also presented bactericidal effect on the culture of P. aeruginosa ATCC 27853. The lowest tested concentrations of the studied Lactobacillus rhamnosus GG (0.03 mg/ml by protein) filtrates and combinations with Saccharomyces boulardii (0.02 mg/ml by protein) caused a decrease in the optical density of the reference strains of S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853 by 77.16–82.30 %, 51.25 – 52.78 %, 31.43–31.58 % (P ≤ 0.01), respectively. The MICs values corresponded to the MBCs.
Conclusions. As a result of the studies, the sensitivity of S. aureus ATCC 25923, E. coli ATCC 25922, and P. aeruginosa ATCC 27853strains to both structural-metabolic complexes of lactobacteria and saccharomycetes was determined. The obtained minimum inhibitory/bactericidal concentrations of the studied filtrates of L. rhamnosus GG and S. boulardii will be useful in the development of promising antimicrobial agents for alternative or add-on therapies of diseases having various origins.
References
Balabekyan, T. R., Karapetyan, K. J., Khachatryan, T. V., Khachatryan, G. E., & Tatikyan, S. S. (2018). Antimicrobial activity of preparations after combined cultivation of lactic acid bacteria and yeast strains. Journal of animal physiology and animal nutrition, 102(4), 933-938. https://doi.org/10.1111/jpn.12891
Isayenko, O. Y., Knysh, O. V., Babych, Y. M., Ryzhkova, T. N., & Dyukareva, G. I. (2019). Effect of disintegrates and metabolites of Lactobacillus rhamnosus and Saccharomyces boulardii on biofilms of antibiotic resistant conditionally pathogenic and pathogenic bacteria. Regulatory Mechanisms in Biosystems, 10(1), 3-8. https://doi.org/10.15421/021901
Sahib, F. H., Aldujaili, N. H., & Alrufae, M. M. (2017). Biosynthesis of silver nanoparticles using Saccharomyces boulardii and study their biological activities. European journal of pharmaceutical and medical research, 4(9), 65-74.
Sarika, A. R., Lipton, A. P., & Aishwarya, M. S. (2010). Bacteriocin Production by a New Isolate of Lactobacillus rhamnosus GP1 under Different Culture Conditions. Advance Journal of Food Science and Technology, 2(5), 291-297.
Semenov, A. V. (2013). Antagonizm kak rezul'tat mezhmikrobnykh otnoshenii [Antagonism as a result cross-species interaction between micro-organisms]. Byulleten' Orenburgskogo nauchnogo tsentra UrO RAN (elektronnyi zhurnal), (1), 1-8. [in Russian].
Isaienko, O. Yu., Knysh, O. V., Babych, Ye. M., Kivva, F. V., Horbach, T. V., & Balak, O. K. (2018). Sposib oderzhannia metabolitiv probiotychnykh shtamiv bakterii [The method of obtaining metabolites of probiotic strains of bacteria]. Ukraine Patent UA 123122. https://base.uipv.org/searchINV/search.php?action=viewdetails&dbname=inv&lang=ukr&chapter=biblio&sortby=_
Usenko, D. V. (2011). K voprosu o roli probioticheskikh produktov v profilaktike zabolevanii i sokhranenii zdorov'ya cheloveka [On the role of probiotic products in disease prevention and maintaining human health]. Lechashchii vrach, (7). https://www.lvrach.ru/2011/07/15435244/ [in Russian].
Lim, P. L., Toh, M., & Liu, S. Q. (2015). Saccharomyces cerevisiae EC-1118 enhances the survivability of probiotic Lactobacillus rhamnosus HN001 in an acidic environment. Applied microbiology and biotechnology, 99(16), 6803-6811. https://doi.org/10.1007/s00253-015-6560-y
Isaienko, O. Yu., Knysh, O. V., Babych, Ye. M., Zachepylo, S. V., Polianska, V. P., Vashchenko, V. L., Kovalenko, O. I., & Balak, O. K. (2018). Sposib kombinatsii metabolitiv probiotychnykh shtamiv hrybiv i bakterii [Method of obtaining a combination of metabolites of probiotic strains of fungi and bacteria]. Ukraine Patent UA 126603. https://base.uipv.org/searchINV/search.php?action=viewdetails&IdClaim=248674
EUCAST. (n.d.). Antimicrobial susceptibility testing. https://www.eucast.org/ast_of_bacteria/
EUCAST. (n.d.). MIC and zone diameter distributions and ECOFFs. https://www.eucast.org/mic_distributions_and_ecoffs/
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. The Journal of biological chemistry, 193(1), 265-275.
Sudagidan, M., & Yemenicioğlu, A. (2012). Effects of nisin and lysozyme on growth inhibition and biofilm formation capacity of Staphylococcus aureus strains isolated from raw milk and cheese samples. Journal of food protection, 75(9), 1627-1633. https://doi.org/10.4315/0362-028X.JFP-12-001
Isayenko, O. Y. (2019). Protydyfteriini vlastyvosti strukturnometabolitnykh kompleksiv probiotychnykh shtamiv laktobakterii i sakharomitsetiv u testakh in vitro ta in vivo [Anti-diphtheria properties of structural-metabolites complexes of Lactobacteria and Saccharomyces probiotic strains]. Fiziolohichnyi zhurnal, 65(6), 51-60. https://doi.org/10.15407/fz65.06.051 [in Ukrainian].
Isayenko, O. Y., Knysh, O. V., Kotsar, O. V., Ryzhkova, T. N., & Dyukareva, G. I. (2019). Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria. Regulatory Mechanisms in Biosystems, 10(2), 245-250. https://doi.org/10.15421/021937
Bengtsson, T., Lönn, J., Khalaf, H., & Palm, E. (2018). The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria-induced proinflammatory responses in dermal fibroblasts. MicrobiologyOpen, 7(6), Article e00606. https://doi.org/10.1002/mbo3.606
Severina, E., Severin, A., & Tomasz, A. (1998). Antibacterial efficacy of nisin against multidrug-resistant Gram-positive pathogens. Journal of antimicrobial chemotherapy, 41(3), 341-347. https://doi.org/10.1093/jac/41.3.341
Sambanthamoorthy, K., Feng, X., Patel, R., Patel, S., & Paranavitana, C. (2014). Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens. BMC Microbiology, 14, Article 197. https://doi.org/10.1186/1471-2180-14-197
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
