Quantification of naphazoline nitrate by UV-spectrophoto-metry

Authors

  • O. I. Panasenko Zaporizhzhia State Medical University,
  • A. A. Safonov Zaporizhzhia State Medical University,
  • A. S. Gotsulya Zaporizhzhia State Medical University,

DOI:

https://doi.org/10.14739/2310-1210.2013.6.20989

Keywords:

naphazoline, substance, nasal drops, UV-spectrophotometry

Abstract

One of the main tasks of pharmaceutical chemistry – medical drugs study. Spectrophotometry is widely used in studying of the structure and composition (complexes, dyes, analytical reagents, etc.) of various compounds. It widely used for qualitative and quantitative determination of substances (determination of elements traces in metals, alloys, technical facilities). The dependence between substance structure and its electronic spectrum is being studied by many researchers till nowadays.

The aim of this work was to highlight the issues of naphazoline quantify definition  techniques by the UV-spectrophotometry. According to the existing methods of quality control (MQC), naphazoline nitrate is a substance quantitatively determined by acid-base titration among a mixture of anhydrous acetic acid and acetic anhydride. Titration is carried out with a solution of  0,1 M perchloric acid (indicator - crystal violet). To check the quality of nasal drops nafazoline nitrate MQC is recommended UV-spectrophotometry: drug is dissolved in boric acid solution (20 g/l) as the reference solution used solution pharmacopoeia standard sample substance nafazoline nitrate. The character of UV-spectra of the nafazoline nitrate in solvents of different polarity (water, 95% ethanol, 0,1 M NaOH, 0,1 M HCl, 5M H2SO4), was defined and studied. Standard sample of nafazoline nitrate was obtained from the State Enterprise "Scientific and Expert Pharmacopoeia Centre Ukraine". In order to study UV-spectra nafazoline nitrate spectrophotometer SPECORD 200-222U214 (Germany) was used.

UV-spectrum of nafazoline nitrate in water and 95% ethanol are characterized by two maxima at 270 and 280 nm. Absorption band of nafazoline nitrate in 0, 1 M sodium hydroxide has two maxima at 271 and 280 nm, and in 0, 1 M solution of hydrochloric acid and 5 M solution of sulfuric acid maxima coincide with the maxima spectrum of the drug in water, 95% ethanol.

In order to avoid errors associated with calibration spectrophotometer, temperature factor pharmacopoeia analysis requires an analysis medications using standard samples analyzed substance. Found that the UV-spectra nafazoline nitrate in these solvents is characterized by one absorption band with two maxima within 270-280 nm. Developed and proposed methods of quantitative determination of nafazoline nitrate in substance and 0,1% nasal drops.

Author Biographies

O. I. Panasenko, Zaporizhzhia State Medical University

Chair of toxicological and inorganic chemistry

A. A. Safonov, Zaporizhzhia State Medical University

Chair of toxicological and inorganic chemistry

A. S. Gotsulya, Zaporizhzhia State Medical University

Chair of toxicological and inorganic chemistry

References

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How to Cite

1.
Panasenko OI, Safonov AA, Gotsulya AS. Quantification of naphazoline nitrate by UV-spectrophoto-metry. Zaporozhye medical journal [Internet]. 2014Jan.29 [cited 2024Apr.25];15(6). Available from: http://zmj.zsmu.edu.ua/article/view/20989

Issue

No. 6 (2013): Zaporozhye medical journal

Section

Problems of pharmacy

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