Association study between BGLAP RS1800247-polymorphic variant and type 2 diabetes mellitus development among hypertensive and non-hypertensive Ukrainians

Authors

DOI:

https://doi.org/10.14739/2310-1210.2021.3.220651

Keywords:

type 2 diabetes mellitus, hypertension, osteocalcin, single nucleotide polymorphism, TT-homozygote, TC-heterozygote, CC-homozygote

Abstract

The risk T2DM development depends on hereditary predisposition. According to the current data, skeleton enhances insulin gene expression in pancreatic β-cells as well as increases insulin sensitivity of adipocytes, myocytes and hepatocytes through the secretion of unOCN.

The aim. To analyze the link between rs1800247 SNP and T2DM occurrence depending on the AH presence, as well as association between rs1800247 and systolic, diastolic, pulse, mean blood pressure among patients with diabetes.

Materials and Methods. This study included 153 patients with diagnosed T2DM and 311 individuals without any carbohydrate metabolism disorders. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) was used for BGLAP rs1800247-genotyping. Logistic regression with interaction term “genotype × AH” was used to estimate the association between BGLAP rs1800247-genotypes and T2DM development under dominant, recessive, over-dominant and additive models of inheritance. Linear regression was performed to investigate the influence of minor C-allele on the arterial blood pressure. All calculations were performed using Statistical Package for the Social Sciences software (SPSS, version 22.0, Chicago, IL, USA). A value of P ˂ 0,05 was considered as significant.

Results. No association was found between rs1800247 single nucleotide polymorphism and T2DM development neither in AH patients, nor in subjects without AH (Paint b > 0,05). There was no impact of rs1800247 genotypes on systolic, diastolic, pulse and mean blood pressure among patients with T2DM (P > 0,05).

Conclusion. It was found the lack of association between rs1800247 SNP and T2DM development as well as blood pressure parameters. Further studies with extended groups of comparison are needed for the confirmation of results. This research was a part of the scientific project “Molecular-genetic and morphological features of lower limb tissues regeneration under conditions of chronic hyperglycemia” (0117U003926).

Author Biographies

Ya. D. Chumachenko, Sumy State University, Ukraine

MD, PhD student

M. O. Kyrychenko, Sumy State University, Ukraine

MD, Intern

D. M. Ramazanova, Kyiv Regional Perinatal Center, Ukraine

MD, Intern, obstetrician-gynecologist

M. V. Smiianova, Medical Center “Medeya”, Sumy, Ukraine

Medical Director

V. Yu. Harbuzova, Sumy State University, Ukraine

MD, PhD, DSc, Professor of the Department of Physiology and Pathophysiology with Medical Biology Course

O. V. Ataman, Sumy State University, Ukraine

MD, PhD, DSc, Professor, Head of the Department of Physiology and Pathophysiology with Medical Biology Course

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Published

2021-06-07

How to Cite

1.
Chumachenko YD, Kyrychenko MO, Ramazanova DM, Smiianova MV, Harbuzova VY, Ataman OV. Association study between BGLAP RS1800247-polymorphic variant and type 2 diabetes mellitus development among hypertensive and non-hypertensive Ukrainians. Zaporozhye medical journal [Internet]. 2021Jun.7 [cited 2024Mar.28];23(3):338-42. Available from: http://zmj.zsmu.edu.ua/article/view/220651

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Original research