Development of methods for standardization of the active substance, namely the model mixture based on decamethoxine and thiotriazoline
DOI:
https://doi.org/10.14739/2310-1210.2021.5.225459Keywords:
decamethoxine, thiotriazoline, model mixtures, spectrophotometryAbstract
Today, the important problem is the creation of new decamethoxine and thiotriazoline based drugs for the treatment of stomatitis.
The aim of the work is to develop a method for quantitative determination of decamethoxine and thiotriazoline in the model mixture (1:25) by spectrophotometry.
Materials and methods. Series 6 model mixtures were made at the ratio of decamethoxine and thiotriazoline 1:25. We used certified substances: thiotriazoline series GTT 3460911 (manufacturer: State Enterprise Chemical Reagents Plant, Kharkiv), decamethoxine series № 010915 (manufacturer: LLC “PHARMCHIM”). Optizen POP spectrophotometer, polyvinyl alcohol, hydrochloric acid, and eosin were used.
Results. A method for quantitative determination of decamethoxine and thiotriazoline in MS was developed. It was established that the content of active substances in MS is thiotriazoline from 0.5021 to 0.5096, decamethoxine from 0.0207 to 0.0211.
Conclusions. A method for quantitative determination of decamethoxine and thiotriazoline in MS has been developed. The method of quantitative determination of decamethoxine and thiotriazoline in MS is reproducible.
References
State Enterprise Ukrainian Scientific Pharmacopoeial Center of Medicines Quality. (2015). Derzhavna Farmakopeia Ukrainy [The State Pharmacopoeia of Ukraine] (2nd ed., Vol. 1). Kharkiv: Naukovo-ekspertnyi farmakopeinyi tsentr. [in Ukrainian].
Grizodub, A. I., Evtifeeva, O. A., Proskurina, K. I., & Bezumova, O. V. (2014). Standartizovannaya protsedura validatsii spektrofotometricheskikh metodik kolichestvennogo opredeleniya lekarstvennykh sredstv v variante metoda pokazatelya pogloshcheniya. Soobshchenie 1 [A standardized validation procedure of the spectrophotometric method for the quantitative determination of drugs in a variant of the absorption index method. Report 1]. Farmakom, (1), 29-39. [in Russian].
Chonka, H. (2019). The problem of the spread diseases of oral mucosa and treatment options. The 23d International Medical Congress of Students and Young Scientists (p. 234). UKRMEDKNYHA. http://dspace.zsmu.edu.ua/bitstream/123456789/11410/1/Chonka234.pdf
Georgievskii, V. P. (Ed.). (2011). Analiticheskaya khimiya v sozdanii, standartizatsii i kontrole kachestva lekarstvennykh sredstv [Analytical chemistry in the discovery, standardization and quality control of medicines] (Vol. 1). NTMT. [in Russian].
Verkhovna Rada of Ukraine. (1996, April 04). Zakon Ukrainy «Pro likarski zasoby» [Law of Ukraine «On Medicinal Products» (No. 123/96-VR)]. https://zakon.rada.gov.ua/laws/show/123/96-%D0%B2%D1%80#Text
Danilevskii, N. F, & Borisenko, A. V. (2000). Zabolevaniya parodonta [Periodontal disease]. Zdorov'e. [in Russian].
Nwizu, N., Wactawski-Wende, J., & Genco, R. J. (2020). Periodontal disease and cancer: Epidemiologic studies and possible mechanisms. Periodontology 2000, 83(1), 213-233. https://doi.org/10.1111/prd.12329
Kucherenko, L. I., Belenichev, I. F., & Chonka, O. O. (2019). A study of the antimicrobial and fungicidal activity of a potentially new combined tablet preparation based on decamethoxin and thiotriazolin. Technological and biopharmaceutical aspects of the drug discovery with different mechanisms of action. Proceedings of the International Scientific and Practical Internet Conference (pp. 12-13). Kharkiv. http://dspace.zsmu.edu.ua/handle/123456789/11750
Zubatyuk, R. I., Kucherenko, L. I., Mazur, I. A., Khromyleva, O. V., & Shishkin, O. V. (2014). A Theoretical Structural Study of Isoniazid Complexes with Thiotriazoline. Chemistry of Heterocyclic Compounds, 50, 438-443 https://doi.org/10.1007/s10593-014-1493-4
Sánchez-Bernal, J., Conejero, C., & Conejero, R. (2020). Recurrent Aphthous Stomatitis. Aftosis oral recidivante. Actas Dermo-Sifiliograficas, 111(6), 471-480. https://doi.org/10.1016/j.ad.2019.09.004
Sawada, S., Chosa, N., Ishisaki, A., & Naruishi, K. (2013). Enhancement of gingival inflammation induced by synergism of IL-1β and IL-6. Biomedical Research, 34(1), 31-40. https://doi.org/10.2220/biomedres.34.31
Mossey, P. A., & Modell, B. (2012). Epidemiology of Oral Clefts 2012: An International Perspective. In M. T. Cobourne (Ed.), Cleft Lip and Palate. Epidemiology, Aetiology and Treatment (Vol. 16, pp. 1-18). Frontiers of Oral Biology. Basel, Karger. https://doi.org/10.1159/000337464
Kinane, D. F., Stathopoulou, P. G., & Papapanou, P. N. (2017). Periodontal diseases. Nature Reviews Disease Primers, 3, Article 17038. https://doi.org/10.1038/nrdp.2017.38
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