The influence of oral antimicrobial peptide content on the quantitative microflora composition in periodontal pockets among residents of a large industrial region

Authors

  • I. V. Vozna Zaporizhzhia State Medical University, Ukraine, Ukraine https://orcid.org/0000-0001-7708-4159
  • S. V. Pavlov Zaporizhzhia State Medical University, Ukraine, Ukraine
  • O. V. Voznyi Zaporizhzhia State Medical University, Ukraine, Ukraine

DOI:

https://doi.org/10.14739/2310-1210.2021.3.229655

Keywords:

periodontopathogenic microflora, periodontitis, polymerase chain reaction

Abstract

The aim was to study the influence of periodontal pocket microecology state on the local nonspecific resistance in steelworkers with generalized periodontitis.

Materials and methods. In total, 178 patients were examined. The study group consisted of 126 patients with generalized periodontitis of initial (n = 8), I (n = 32), II (n = 68) and III (n = 18) degree of severity, chronic course, exposed to work-related hazardous agents. The comparison group consisted of 32 patients with periodontitis of initial (n = 5), I (n = 10), II (n = 11) and III (n = 6) degree of severity without exposure to harmful conditions of steel industry. The control group included 20 otherwise healthy individuals. Detection of the main five periodontopathogenic microorganisms in the crevicular fluid was carried out by the polymerase chain reaction method. The levels of lactoferrin and cathelicidin LL-37 were measured by the enzyme-linked immunosorbent assay method.

Results. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were less common, than other opportunistic bacteria in both study groups with initial severity of generalized periodontitis. With the disease development, there was a tendency to increase the number of positive samples for all microorganisms. A correlation was found between the microorganism presence in the sample and the severity of periodontal disease. In the patients of clinical groups, the concentration of cathelicidin LL-37 in the oral fluid was reduced. In the study group, a moderate inverse correlation between cathelicidin and Porphyromonas gingivalis as well as a weak inverse correlation between cathelicidin and Prevotella intermedia were revealed. Lactoferrin was correlated directly and moderately with Porphyromonas gingivalis and Prevotella intermedia but weakly - with Treponema denticola. In the comparison group, there were a statistically significant moderate inverse correlation between cathelicidin and Porphyromonas gingivalis as well a direct correlation between lactoferrin and Porphyromonas gingivalis. Correlations between the concentrations of cathelicidin and lactoferrin in the oral fluid and other periodontopathogens were revealed to be weak.

Conclusions. The studies have shown significant disruptions of the oral cavity microecology in the patients exposed to harmful effects of steel industry. The correlation between the concentration of antimicrobial peptides and periodontal pathogens in the periodontal pockets has been found.

Author Biographies

I. V. Vozna, Zaporizhzhia State Medical University, Ukraine

MD, PhD, Associate Professor of the Department of Surgical and Propaedeutic Dentistry

S. V. Pavlov, Zaporizhzhia State Medical University, Ukraine

PhD, DSc, Associate Professor, Head of the Department of Clinical Laboratory Diagnostics

O. V. Voznyi, Zaporizhzhia State Medical University, Ukraine

MD, PhD, DSc, Professor, Head of the Department of Therapeutic, Orthopedic and Pediatric Dentistry

References

Romanova, Yu. H., Zahradska, E. L., & Strochenko, E. A. (2016). Vliyanie neblagopriyatnykh faktorov okruzhayushchei sredy na sostoyanie gomeostaza u patsientov so stomatologicheskoi patologiei [Impact of unfavourable factors of environment on homeostasis in patients with dental pathology]. Ukrainskyi stomatolohichnyi almanakh, (1), 33-36. [in Russian].

Savel'eva, N. M., Sokolova, I. I., German, S. I., & Tomilina, T. V. (2018). Imunolohichni aspekty heneralizovanoho parodontytu (ohliad literatury) [Immunological aspects of generalized periodontitis (literature review)]. Visnyk naukovykh doslidzhen, (2), 110-115. https://doi.org/10.11603/2415-8798.2018.2.9122 [in Ukrainian].

Popova, V. S., Suschenko, A. V., & Vusataya, E. V. (2017). Osobennosti stomatologicheskogo statusa rabotnikov metallurgicheskogo proizvodstva [Peculiarities of stomatological status of metallurgical manufacturing workers]. Mezhdunarodnyi nauchno-issledovatel'skii zhurnal, (11. Pt. 3), 39-42. https://doi.org/10.23670/IRJ.2017.65.137 [in Russian].

Chubij, I. Z., & Rozhko, M. M. (2015). Likuvannia heneralizovanoho parodontytu pochatkovoho stupenia rozvytku v osib, yaki prozhyvaiut na ekolohichno zabrudnenykh terytoriiakh Prykarpattia [Treatment of generalized periodontitis of initial stage in people who lives in polluted areas of Precarpathians]. Ukrainskyi stomatolohichnyi almanakh, (6), 5-7. [in Ukrainian].

Gruzdeva, A. A., & Glazunov, O. A. (2016). Sostoyanie tkanei parodonta u robochikh zhelezorudnogo proizvodstva (obzor literatury) [State of fabrics of paradontium for the workers of iron-ore production (review of the literature)]. Ukrainskyi stomatolohichnyi almanakh, (4), 99-103. [in Russian].

Pupin, T. I., Nemesh, O. M., Honta, Z. M., Shylivskyi, I. V., Moroz, K. A., & Bumbar, O. I. (2020). Suchasni aspekty likuvannia heneralizovanoho parodontytu v osib iz somatychnoiu patolohiieiu [Modern aspects of generalized periodontitis treatment in patients with a somatic pathology]. Zaporozhye medical journal, 22(1), 122-128. https://doi.org/10.14739/2310-1210.2020.1.194649 [in Ukrainian].

Tsarev, V. N., Nikolaeva, E. N., & Ippolitov, E. V. (2017). Parodontopatogennye bakterii - osnovnoi faktor vozniknoveniya i razvitiya parodontita [Periodontophatogenic bacteria of the main factors of emergence and development of periodontitis]. Zhurnal mikrobiologii, epidemiologii i immunobiologii, (5), 101-112. https://doi.org/10.36233/0372-9311-2017-5-101-112 [in Russian].

Perebeynos, O. P., Ostrovsky, P. Yu., & Boytsanyuk, S. I. (2015). Rol bakterii pry zakhvoriuvanniakh tkanyn parodonta [Role of bacteria in disease of periodontal tissues]. Molodyi vchenyi, (2), 648-651. [in Ukrainian].

Tamarova, E. R., Shvets, K. Yu., Mavzyutov, A. R., Baimiev, Al. Kh., & Bulyakov, R. T. (2016). Ispol'zovanie metoda polimernoi tsepnoi reaktsii v rezhime real'nogo vremeni dlya vidovoi kharakteristiki mikrobioty polosti rta i otsenki effektivnosti terapii pri parodontite [Use of pcr in real time for specific characteristics of oral cavity microbiota and estimation of therapy efficiency in periodontitis]. Meditsinskii vestnik Bashkortostana, 11(2), 19-23. [in Russian].

Usmanovа, I. N., Tuygunov, M. M., Gerasimova, L. P., Kabirova, M. F., Gubaydullin, A. G., Gerasimova, A. A., & Chusnarisanova, R. F. (2015). Rol' uslovno-patogennoi mikroflory polosti rta v razvitii vospalitel'nykh zabolevanii parodonta i slizistoi polosti rta (obzor literatury) [Role of opportunistic oral microflora in the development of inflammatory diseases of periodontal and oral mucosa (review)]. Vestnik YuUrGU. Seriya «Obrazovanie, zdravookhranenie, fizicheskaya kul'tura», 15(2), 37-44. https://doi.org/10.14529/ozfk150207 [in Russian].

Zaydullin, I. I., Karimov, D. O., Kabirova, M. F., Valeeva, E. T., & Galimova, R. R. (2018). Otsenka rasprostranennosti osnovnykh parodontopatogenov u rabotnikov neftekhimicheskogo proizvodstva s khronicheskim parodontitom [Estimation of prevalence of main periodontal pathogens at workers of petrochemical production with chronic periodontal diseases]. Problemy stomatologii, 14(2), 19-24. https://doi.org/10.18481/2077-7566-2018-14-2-19-24 [in Russian].

van't Hof, W., Veerman, E. C., Nieuw Amerongen, A. V., & Ligtenberg, A. J. (2014). Antimicrobial Defense Systems in Saliva. In A. J. M. Ligtenberg & E. C. I. Veerman (Eds.), Monographs in oral science (Vol. 24, pp. 40-51). https://doi.org/10.1159/000358783

Yong, X., Chen, Y., Tao, R., Zeng, Q., Liu, Z., Jiang, L., Ye, L., & Lin, X. (2015). Periodontopathogens and human β-defensin-2 expression in gingival crevicular fluid from patients with periodontal disease in Guangxi, China. Journal of Periodontal Research, 50(3), 403-410. https://doi.org/10.1111/jre.12220

Birsan, I. (2015). Polymerase chain reaction as a prospect for the early diagnosis and prediction of periodontal diseases in adolescents. European Archives of Paediatric Dentistry, 16(1), 9-12. https://doi.org/10.1007/s40368-014-0138-8

Kumawat, R. M., Ganvir, S. M., Hazarey, V. K., Qureshi, A., & Purohit, H. J. (2016). Detection of Porphyromonas gingivalis and Treponema denticola in chronic and aggressive periodontitis patients: A comparative polymerase chain reaction study. Contemporary Clinical Dentistry, 7(4), 481-486. https://doi.org/10.4103/0976-237X.194097

Rafiei, M., Kiani, F., Sayehmiri, K., Sayehmiri, F., Tavirani, M., Dousti, M., & Sheikhi, A. (2018). Prevalence of Anaerobic Bacteria (P.gingivalis) as Major Microbial Agent in the Incidence Periodontal Diseases by Meta-analysis. Journal of Dentistry, 19(3), 232-242.

Kang, H. K., Kim, C., Seo, C. H., & Park, Y. (2017). The therapeutic applications of antimicrobial peptides (AMPs): a patent review. Journal of Microbiology, 55(1), 1-12. https://doi.org/10.1007/s12275-017-6452-1

Horie, T., Inomata, M., & Into, T. (2018). OmpA-Like Proteins of Porphyromonas gingivalis Mediate Resistance to the Antimicrobial Peptide LL-37. Journal of Pathogens, 2018, Article 2068435. https://doi.org/10.1155/2018/2068435

Legrand, D. (2016). Overview of Lactoferrin as a Natural Immune Modulator. The Journal of Pediatrics, 173, S10-S15. https://doi.org/10.1016/j.jpeds.2016.02.071

Velliyagounder, K., Bahdila, D., Pawar, S., & Fine, D. H. (2019). Role of lactoferrin and lactoferrin-derived peptides in oral and maxillofacial diseases. Oral Diseases, 25(3), 652-669. https://doi.org/10.1111/odi.12868

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Published

2021-06-07

How to Cite

1.
Vozna IV, Pavlov SV, Voznyi OV. The influence of oral antimicrobial peptide content on the quantitative microflora composition in periodontal pockets among residents of a large industrial region. Zaporozhye Medical Journal [Internet]. 2021Jun.7 [cited 2024Dec.25];23(3):388-94. Available from: http://zmj.zsmu.edu.ua/article/view/229655

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Original research