Detection of serum antibodies to tick-borne and other infections in patients with lymphadenopathy
DOI:
https://doi.org/10.14739/2310-1210.2022.1.238413Keywords:
lymph nodes, Lyme borreliosis, human granulocytic anaplasmosis, other infectionsAbstract
The aim was to determine the prevalence of serum antibodies to tick-borne pathogens and other infectious agents in patients with lymphadenopathy.
Materials and methods. 68 patients with lymphadenopathy aged 20 to 63 years who received outpatient or inpatient treatment were followed up at Ternopil University Hospital and Ternopil Regional Clinical Dermatological and Venereological Dispensary. There were 41 women (60.3 %) and 27 men (39.7 %).
Two-stage serological detection (ELISA and immunoblot) was used for diagnosis of Lyme borreliosis (LB). Indirect immunofluorescence, including the BIOCHIP multiplex indirect immunofluorescence technique, was used for serological identification of human granulocytic anaplasmosis (HGA) and other infectious diseases, which could be associated with lymphadenopathy.
Results. In total, 45.6 % of 68 enrolled patients were positive for the presence of specific antibodies IgM and/or IgG against B. burgdorferi s. l. Further examination demonstrated the presence of specific serum anti-A. phagocytophilum antibodies IgM and/or IgG in 33 patients with lymphadenopathy.
It was found that 24.2 % of involved patients were positive for the presence of at least one type of anti-A. phagocytophilum antibodies; 12.1 % had positive or intermediate results only for B. burgdorferi s. l.; 60.6 % of patients were positive for the presence of antibodies against both pathogens.
46.9 % of patients were seropositive for EBV-CA, 40.6 % – for Parainfluenza virus type 1, 37.5 % – for HHV-6, 34.4 % – for HSV-1, 31.3 % – for EBV-EA and Toxoplasma gondii, 6.3 % – for Coxsackievirus type A9 and HIV-2.
Conclusions. One-third of all involved patients with lymphadenopathy were positive for antibodies to B. burgdorferi s. l., causa tive agent of Lyme borreliosis. Co-infection of Lyme borreliosis and human granulocytic anaplasmosis was established in more than half of the patients. Besides, specific antibodies to 18 causative agents of other infectious diseases were detected with varying rate. Almost half of positive results detected were the antibodies to parainfluenza virus type 1 and Epstein–Barr virus.
References
Maini, R., & Nagalli, S. (2021). Lymphadenopathy. In StatPearls. StatPearls Publishing.
Tunev, S. S., Hastey, C. J., Hodzic, E., Feng, S., Barthold, S. W., & Baumgarth, N. (2011). Lymphoadenopathy during Lyme Borreliosis Is Caused by Spirochete Migration-Induced Specific B Cell Activation. PLOS PATHOGENS, 7(5), Article e1002066. https://doi.org/10.1371/journal.ppat.1002066
Koester, T. M., Meece, J. K., Fritsche, T. R., & Frost, H. M. (2018). Infectious Mononucleosis and Lyme Disease as Confounding Diagnoses: A Report of 2 Cases. Clinical Medicine & Research, 16(3-4), 66-68. https://doi.org/10.3121/cmr.2018.1419
Seo, J. W., Kim, C. M., Yun, N. R., Kim, D. M., Kim, S. S., Choi, S., & Chu, H. (2020). Scalp eschar and neck lymphadenopathy after tick bite (SENLAT) caused by Bartonella henselae in Korea: a case report. BMC Infectious Diseases, 20(1), Article 216. https://doi.org/10.1186/s12879-020-4940-0
Stinco, G., & Bergamo, S. (2016). Impact of Co-Infections in Lyme Disease. The Open Dermatology Journal, 10(1), 55-61. https://doi.org/10.2174/1874372201610010055
Klymnyuk, S. I., Romanyuk, L. B., & Shkilna, M. I. (2017). Suchasni uiavlennia pro hranulotsytarnyi anaplazmoz liudyny [Modern views about the granulocytic anaplasmosis of a man]. Infektsiini khvoroby, (3). 4-9. https://doi.org/10.11603/1681-2727.2017.3.8220 [in Ukrainian].
Davidsson, M. (2018). The Financial Implications of a Well-Hidden and Ignored Chronic Lyme Disease Pandemic. Healthcare, 6(1), Article 16. https://doi.org/10.3390/healthcare6010016
Kugeler, K. J., & Eisen, R. J. (2020). Challenges in Predicting Lyme Disease Risk. JAMA Network Open, 3(3), Article e200328. https://doi.org/10.1001/jamanetworkopen.2020.0328
Shkilna, M. I., Andreychyn, M. A., Podobivsky, S. S., Fedoniuk, L. Ya., Panychev, V. A., Ivakhiv, O. L., Vyshnevska, N. Yu., Marchuk, O. M., Korda, M. M., & Klishch, I. M. (2020). Zarazhenist klishchiv, vidibranykh vid liudei v Ukraini, zbudnykamy deiakykh bakterioziv [Infection of ticks collected from humans in Ukraine, by causative agents of some bacteriosis]. Bukovynskyi medychnyi visnyk, 24(1), 195-201. https://doi.org/10.24061/2413-0737.XXIV.1.93.2020.26 [in Ukrainian].
Sormunen, J. J., Penttinen, R., Klemola, T., Hänninen, J., Vuorinen, I., Laaksonen, M., Sääksjärvi, I. E., Ruohomäki, K., & Vesterinen, E. J. (2016). Tick-borne bacterial pathogens in southwestern Finland. Parasites & Vectors, 9, Article 168. https://doi.org/10.1186/s13071-016-1449-x
Wormser, G. P., Aguero-Rosenfeld, M. E., Cox, M. E., Nowakowski, J., Nadelman, R. B., Holmgren, D., McKenna, D., Bittker, S., Zentmaier, L., Cooper, D., Liveris, D., Schwartz, I., & Horowitz, H. W. (2013). Differences and Similarities between Culture-Confirmed Human Granulocytic Anaplasmosis and Early Lyme Disease. Journal of Clinical Microbiology, 51(3), 954-958. https://doi.org/10.1128/JCM.02929-12
Berghoff, W. (2012). Chronic Lyme Disease and Co-infections: Differential Diagnosis. The Open Neurology Journal, 6(Suppl. 1), 158-178. https://doi.org/10.2174/1874205X01206010158
Garg, K., Jokiranta, T. S., Filén, S., & Gilbert, L. (2021). Assessing the Need for Multiplex and Multifunctional Tick-Borne Disease Test in Routine Clinical Laboratory Samples from Lyme Disease and Febrile Patients with a History of a Tick Bite. Tropical Medicine and Infectious Disease, 6(1), Article 38. https://doi.org/10.3390/tropicalmed6010038
Johnson, L., Wilcox, S., Mankoff, J., & Stricker, R. B. (2014). Severity of chronic Lyme disease compared to other chronic conditions: a quality of life survey. PeerJ, 2, Article e322. https://doi.org/10.7717/peerj.322
Tumminello, R., Glaspey, L., Bhamidipati, A., Sheehan, P., & Patel, S. (2017). Early Disseminated Lyme Disease Masquerading as Mononucleosis: A Case Report. The Journal of Emergency Medicine, 53(6), e133-e135. https://doi.org/10.1016/j.jemermed.2017.09.005
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