Dynamics of methicillin-resistant staphylococcus aureus prevalence among patients of the Chernivtsi region
DOI:
https://doi.org/10.14739/2310-1210.2022.4.254912Keywords:
methicillin resistance, sensitivity, prevalence, MRSAAbstract
The aim of this study was to analyze the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) during 2018–2020 among patients with purulent-inflammatory diseases of different localization in the Chernivtsi region.
Materials and methods. A total of 804 strains of S. aureus were studied. Isolates were identified by morphological, tinctorial, physiological and biochemical characteristics, and their sensitivity to antibiotics was tested. Preparation and the susceptibility testing were performed in accordance with the recommendations of the Clinical & Laboratory Standards Institute (2017), as well as quality control of each batch of antibiotic discs (Oxoid, UK). Determination of methicillin resistance was performed by a surrogate test using a disk of 30 μg cefoxitin.
Results. The significant increasing in the frequency of MRSA were found in 2020 as compared to previous years – a total of 1.6–2.0 times compared to 2018 and 2019. The difference between 2019 and 2020 was statistically significant: tφ = 2.49 (P ˂ 0.05). There was also a clear upward tendency in the isolation frequency of poly-resistant strains during the observation period.
Conclusions. Significant changes in the frequency of isolation of Staphylococcus aureus strains from foci of purulent-inflammatory processes of different localization were not detected over the years of observations. There was an increase in the isolation frequency of the MRSA strains from patients with purulent-inflammatory diseases in the Chernivtsi region in 2020 mainly due to isolation of such strains from the mucous membranes of the oral cavity and tonsils. The proportion of MRSA poly-resistant strains increased significantly (almost twice) during the observation period suggesting that their spread should be monitored.
References
World Health Organization. (2017). WHO publishes list of bacteria for which new antibiotics are urgently needed. https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed
Vadivoo Shanmuga, N., & Usha, B. (2018). ESKAPE pathogens: Trends in antibiotic resistance pattern. MedPulse International Journal of Microbiology, 7(3), 26-32. https://doi.org/10.26611/1008732
Lakhundi, S., & Zhang, K. (2018). Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology. Clinical microbiology reviews, 31(4), e00020-18. https://doi.org/10.1128/CMR.00020-18
Gajdács, M. (2019). The Continuing Threat of Methicillin-Resistant Staphylococcus aureus. Antibiotics (Basel), 8(2), 52. https://doi.org/10.3390/antibiotics8020052
Ali, E. A., Alshuaibi, O. N. M., & Ali, K. S. (2021). Evaluation of some antibiotic resistance in staphylococcus aureus isolated by medical laboratories Aden, Yemen. Electronic Journal of University of Aden for Basic and Applied Sciences, 2(1), 49-53. https://doi.org/10.47372/ejua-ba.2021.1.89
Patel, J. B., Weinstein, M. P., Eliopoulos, G. M., Jenkins, S. G., Lewis II, J. S., Limbago, B., Mathers, A. J., Mazzulli, T., Patel, R., Richter, S. S., Satlin, M., Swenson, J. M., Traczewski, M. M., Turnidge J. D., & Zimmer, B. L. (2017). Performance Standards for Antimicrobial Susceptibility Testing. CLSI supplement M100 (27th ed.). Clinical Laboratory Standards Institute.
Polishchuk, N. M., Kyryk, D. L., & Yurchuk, I. Ye. (2021). Mikrobiolohichnyi monitorynh yak skladova efektyvnoi profilaktyky i likuvannia hniino-septychnykh infektsii v umovakh ortopedo-travmatolohichnoho viddilennia [Microbiological monitoring as a component of efficient prevention and treatment of purulent-septic infections in an orthopedics and traumatology department]. Zaporozhye medical journal, 23(3), 381-387. [in Ukrainian]. https://doi.org/10.14739/2310-1210.2021.3.229667
Romaniuk, L. B., Kravets, N. Ya., Klymniuk, S. I., Kopcha, V. S., & Dronova, O. Y. (2020). Antybiotykorezystentnist umovno-patohennykh mikroorhanizmiv: aktualnist, umovy vynyknennia, shliakhy podolannia [Antibiotic-resistance of opportunistic microorganisms: topicality, conditions of emergency, ways of overcome]. Infektsiini khvoroby, (4), 63-71. [in Ukrainian]. https://doi.org/10.11603/1681-2727.2019.4.10965
Salmanov, A. G. (2018). Ukrainian Strategy and Action Plan for the Prevention of Healthcare Association Infections (HAIs) and Antimicrobial Resistance. International Journal of Antibiotics and Probiotics, (2-3), 6-23. https://doi.org/10.31405/ijap.2-3.18.01
Kateete, D. P., Bwanga, F., Seni, J., Mayanja, R., Kigozi, E., Mujuni, B., Ashaba, F. K., Baluku, H., Najjuka, C. F., Källander, K., Rutebemberwa, E., Asiimwe, B. B., & Joloba, M. L. (2019). CA-MRSA and HA-MRSA coexist in community and hospital settings in Uganda. Antimicrobial resistance and infection control, 8, 94. https://doi.org/10.1186/s13756-019-0551-1
Bergey, D. H., & Holt, J. G. (2000). Bergey's Manual of Determinative Bacteriology (9th ed.). Lippincott Williams & Wilkins.
Ai, X., Gao, F., Yao, S., Liang, B., Mai, J., Xiong, Z., Chen, X., Liang, Z., Yang, H., Ou, Z., Gong, S., Long, Y., & Zhou, Z. (2020). Prevalence, Characterization, and Drug Resistance of Staphylococcus Aureus in Feces From Pediatric Patients in Guangzhou, China. Frontiers in medicine, 7, 127. https://doi.org/10.3389/fmed.2020.00127
Deng, L., Schilcher, K., Burcham, L. R., Kwiecinski, J. M., Johnson, P. M., Head, S. R., Heinrichs, D. E., Horswill, A. R., & Doran, K. S. (2019). Identification of Key Determinants of Staphylococcus aureus Vaginal Colonization. mBio, 10(6), e02321-19. https://doi.org/10.1128/mBio.02321-19
Gajdács, M., & Urbán, E. (2019). Epidemiology and resistance trends of Staphylococcus aureus isolated from vaginal samples: a 10-year retrospective study in Hungary. Acta dermatovenerologica Alpina, Pannonica, et Adriatica, 28(4), 143-147.
Cong, Y., Yang, S., & Rao, X. (2019). Vancomycin resistant Staphylococcus aureus infections: A review of case updating and clinical features. Journal of advanced research, 21, 169-176. https://doi.org/10.1016/j.jare.2019.10.005
Dyer, J. (2021). The Long Arm of MRSA. Infection Control Today, 25(9), 20-21.
World Health Organization. (2020). Central Asian and European Surveillance of Antimicrobial Resistance. Annual report 2020. WHO. https://www.euro.who.int/__data/assets/pdf_file/0003/469200/Central-Asian-and-European-Surveillance-of-Antimicrobial-Resistance.-Annual-report-2020-eng.pdf
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