Effect of walnut extract supplemented with carbon dioxide on adhesive activity of agents causing wound infection

Authors

DOI:

https://doi.org/10.14739/2310-1210.2023.2.263966

Keywords:

walnut, plant extracts adhesion, causative agents of purulent-inflammatory infections

Abstract

The aim: to study the effect of 3 % carbon dioxide walnut extract (CDWE) on the adhesive properties of gram-positive and gram-negative microorganisms.

Materials and methods. The objects of the study – 3 % CDWE, museum strains of the ATCC collection and clinical cultures of microorganisms: A. baumannii, S. aureus, E. coli, K. pneumoniae, P. aeruginosa, C. albicans. Native and formalinized human erythrocytes of 0(I) Rh-positive blood group were used as a substrate for adhesion. A smear was prepared from each sample, which was stained by the Romanovsky–Giemsa method. According to the results of smear microscopy, the adhesion index of microorganisms (AIM) was calculated.

Results. A preliminary study of the adhesion ability of museum and clinical strains of microorganisms revealed that all cultures possessed highly adhesive properties. The addition of 3 % CDWE significantly inhibited their adhesive properties. Thus, in studies with museum strains, it was found that the AIM of S. aureus was decreased by 59.47 %, K. pneumonia – by 59.17 %, E. coli – by 56.32 %, P. aeruginosa – by 48.70 %, A. baumannii – by 49.77 %, C. albicans – by 51.59 %. In the experiments, a decrease in AIM of all clinical strains of microorganisms was detected: S. aureus – by 55.00 %, K. pneumonia – by 53.50 %, E. coli – by 53.54 %, P. aeruginosa – by 49.04 %, A. baumannii – by 50.42 %, C. albicans – by 53.13 %.

Conclusions. The results gained from the study of the 3 % CDWE effect on the adhesive activity collectively suggest its high ability to inhibit the adhesive activity of museum and clinical strains of purulent-inflammatory infections (S. aureus, P. aeruginosa, A. baumannii, K. pneumoniae, E. coli, C. albicans) and prevent the formation of a bacterial biofilm. These properties indicate potential benefits from further study on the biological properties of CDWE and the development of new antimicrobial drugs based on it for the treatment of purulent-inflammatory infections.

Author Biographies

I. V. Mozhaiev, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

PhD student

V. Yu. Yevsiukova, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

МD, PhD, Senior Researcher, Leading Researcher of the Laboratory of Antimicrobial Agents

I. I. Torianyk, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

MD, PhD, DSc, Senior Researcher, Leading Researcher of the Laboratory of Viral Infections

V. V. Minukhin, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

MD, PhD, DSc, Professor, Director

N. M. Polishchuck, Zaporizhzhia State Medical University, Ukraine

MD, PhD, Associate Professor of the Department of Microbiology, Virology and Immunology

D. L. Kyryk, Shupyk National Healthcare University of Ukraine, Kyiv

MD, PhD, DSc, Professor, Head of the Department of Microbiology

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Published

2023-03-28

How to Cite

1.
Mozhaiev IV, Yevsiukova VY, Torianyk II, Minukhin VV, Polishchuck NM, Kyryk DL. Effect of walnut extract supplemented with carbon dioxide on adhesive activity of agents causing wound infection. Zaporozhye Medical Journal [Internet]. 2023Mar.28 [cited 2024Jul.23];25(2):158-63. Available from: http://zmj.zsmu.edu.ua/article/view/263966

Issue

Vol. 25 No. 2 (2023): Zaporozhye medical journal

Section

Original research

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