Effect of walnut extract supplemented with carbon dioxide on adhesive activity of agents causing wound infection

Authors

DOI:

https://doi.org/10.14739/2310-1210.2023.2.263966

Keywords:

walnut, plant extracts adhesion, causative agents of purulent-inflammatory infections

Abstract

The aim: to study the effect of 3 % carbon dioxide walnut extract (CDWE) on the adhesive properties of gram-positive and gram-negative microorganisms.

Materials and methods. The objects of the study – 3 % CDWE, museum strains of the ATCC collection and clinical cultures of microorganisms: A. baumannii, S. aureus, E. coli, K. pneumoniae, P. aeruginosa, C. albicans. Native and formalinized human erythrocytes of 0(I) Rh-positive blood group were used as a substrate for adhesion. A smear was prepared from each sample, which was stained by the Romanovsky–Giemsa method. According to the results of smear microscopy, the adhesion index of microorganisms (AIM) was calculated.

Results. A preliminary study of the adhesion ability of museum and clinical strains of microorganisms revealed that all cultures possessed highly adhesive properties. The addition of 3 % CDWE significantly inhibited their adhesive properties. Thus, in studies with museum strains, it was found that the AIM of S. aureus was decreased by 59.47 %, K. pneumonia – by 59.17 %, E. coli – by 56.32 %, P. aeruginosa – by 48.70 %, A. baumannii – by 49.77 %, C. albicans – by 51.59 %. In the experiments, a decrease in AIM of all clinical strains of microorganisms was detected: S. aureus – by 55.00 %, K. pneumonia – by 53.50 %, E. coli – by 53.54 %, P. aeruginosa – by 49.04 %, A. baumannii – by 50.42 %, C. albicans – by 53.13 %.

Conclusions. The results gained from the study of the 3 % CDWE effect on the adhesive activity collectively suggest its high ability to inhibit the adhesive activity of museum and clinical strains of purulent-inflammatory infections (S. aureus, P. aeruginosa, A. baumannii, K. pneumoniae, E. coli, C. albicans) and prevent the formation of a bacterial biofilm. These properties indicate potential benefits from further study on the biological properties of CDWE and the development of new antimicrobial drugs based on it for the treatment of purulent-inflammatory infections.

Author Biographies

I. V. Mozhaiev, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

PhD student

V. Yu. Yevsiukova, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

МD, PhD, Senior Researcher, Leading Researcher of the Laboratory of Antimicrobial Agents

I. I. Torianyk, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

MD, PhD, DSc, Senior Researcher, Leading Researcher of the Laboratory of Viral Infections

V. V. Minukhin, State Institution “Institute of Microbiology and Immunology named after I. I. Mechnikov of the National Academy of Sciences of Ukraine”, Kharkiv

MD, PhD, DSc, Professor, Director

N. M. Polishchuck, Zaporizhzhia State Medical University, Ukraine

MD, PhD, Associate Professor of the Department of Microbiology, Virology and Immunology

D. L. Kyryk, Shupyk National Healthcare University of Ukraine, Kyiv

MD, PhD, DSc, Professor, Head of the Department of Microbiology

References

Faustova, M. O. (2021). Vzaiemozviazok adhezyvnykh ta plivkoutvoriuiuchykh vlastyvostei Staphylococcus spp. – zbudnykiv infektsiyno-zapalnykh zakhvoriuvan myakykh tkanyn shchelepno-lytsevoi dilianky [Relationship between adhesive and film-forming properties of Staphylococcus spp. - pathogens of infectious inflammatory diseases in soft tissues of jaw and facial area]. Aktualni problemy suchasnoi medytsyny, 21(2), 165-168. [in Ukrainian]. https://doi.org/10.31718/2077-1096.21.2.165

Krachler, A. M., & Orth, K. (2013). Targeting the bacteria-host interface: strategies in anti-adhesion therapy. Virulence, 4(4), 284-294. https://doi.org/10.4161/viru.24606

Minukhin, V. V., Kochneva, O. V., Gromatiuk, S. M., & Sukhomlyn, M. P. (2013). Adhezyvni vlastyvosti asotsiatsiyi Candida albicans i Staphylococcus aureus [Adhesive Propeties of Association Candida Albicans and Staphylococcus Aureus]. Visnyk problem biolohii i medytsyny, (2), 89-91. [in Ukrainian].

Vrynchanu, N. O., Dudikova, D. M., Hrynchuk, N. I., & Nedashkivska, V. V. (2019). Bioplivky. Suchasnyi stan i perspektyvy antymikrobnoi terapii [Biofilms. The current status and prospects of antimicrobial therapy]. Farmakolohiia ta likarska toksykolohiia, 13(5), 311-321. [in Ukrainian]. https://doi.org/10.33250/13.05.311

World Health Organization. (2017). WHO publishes list of bacteria for which new antibiotics are urgently needed. https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed

Minukhin, V., Kazmirchuk, V., & Torianyk, I. (2019). Likarski kompozytsii na osnovi nastoianok/ ekstraktiv horikhiv chornoho/voloskoho u infektsinii patolohii ran ta ranovykh uskladnen [Pharmaceutical compositions based on infusions/extracts of black walnuts/walnut in infectious pathology and wound healing complications]. Medical sciences, 1(1), 59-69. [in Ukrainian]. https://doi.org/10.30525/978-9934-588-15-0-68

Fernández-Agulló, A., Pereira, E., Freirea, M. S., Valentão, P., Andrade, B., González-Álvarez, J., & Pereira, J. A. (2013). Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts. Industrial Crops and Products, 42(1), 126-132. https://doi.org/10.1016/j.indcrop.2012.05.021

Nozohour, Y., Golmohammadi, R., Mirnejad, R., Moghaddam, M. M., & Fartashvand, M. (2019). Comparison of Antibacterial Activities of Walnut (Juglans Regia L.) and Pine (Pinus halepensis Mill.) Leaves Alcoholic Extracts against Bacteria Isolated from Burn Wound Infections. Acta Microbiologica Hellenica, 64(2), 99-108.

Jahanban-Esfahlan, A., Ostadrahimi, A., Tabibiazar, M., & Amarowicz, R. (2019). A Comparative Review on the Extraction, Antioxidant Content and Antioxidant Potential of Different Parts of Walnut (Juglans regia L.) Fruit and Tree. Molecules, 24(11), 2133. https://doi.org/10.3390/molecules24112133

Genovese, C., Cambria, M. T., D’angeli, F., Addamo, A. P., Malfa, G. A., Siracusa, L., Pulvirenti, L., Anfuso, C. D., Lupo, G., & Salmeri, M. (2020). The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth. International Journal of Oncology, 57(5), 1129-1144. https://doi.org/10.3892/ijo.2020.5130

D’Angeli, F., Malfa, G. A., Garozzo, A., Volti, G. Li, Genovese, C., Stivala, A., Nicolosi, D., Attanasio, F., Bellia, F., Ronsisvalle, S., & Acquaviva, R. (2021). Antimicrobial, Antioxidant, and Cytotoxic Activities of Juglans regia L. Pellicle Extract. Antibiotics, 10(2), 159. https://doi.org/10.3390/antibiotics10020159

Nasiry, D., Khalatbary, A. R., Ahmadvand, H., Amiri, F. B. T., & Akbari, E. (2017). Protective effects of methanolic extract of Juglans regia L. leaf on streptozotocin-induced diabetic peripheral neuropathy in rats. BMC Complementary and Alternative Medicine, 17(1), 476. https://doi.org/10.1186/s12906-017-1983-x

Essa, M. M., Subash, S., Dhanalakshmi, C., Manivasagam, T., Al-Adawi, S., Guillemin, G.J., & Justin Thenmozhi, A. (2015). Dietary Supplementation of Walnut Partially Reverses 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Induced Neurodegeneration in a Mouse Model of Parkinson’s Disease. Neurochemical Research, 40(6), 1283-1293. https://doi.org/10.1007/s11064-015-1593-2

Muthaiyah, B., Essa, M. M., Chauhan, V., & Chauhan, A. (2011). Protective effects of walnut extract against amyloid beta peptide-induced cell death and oxidative stress in PC12 cells. Neurochemical research, 36(11), 2096-2103. https://doi.org/10.1007/s11064-011-0533-z

Nasiry, D., Khalatbary, A. R., Ahmadvand, H., & Talebpour Amiri, F. (2019). Juglans Regia L. Leaf Extract Attenuates Diabetic Nephropathy Progression in Experimental Diabetes: An Immunohistochemical Study. Iranian journal of medical sciences, 44(1), 44-52.

Zhao, M. H., Jiang, Z. T., Liu, T., & Li, R. (2014). Flavonoids in Juglans regia L. leaves and evaluation of in vitro antioxidant activity via intracellular and chemical methods. Scientific World Journal, 2014, 303878. https://doi.org/10.1155/2014/303878

Wu, S., Ni, Z., Wang, R., Zhao, B., Han, Y., Zheng, Y., Liu, F., Gong, Y., Tang, F., & Liu, Y. (2020). The effects of cultivar and climate zone on phytochemical components of walnut (Juglans regia L.). Food and Energy Security, 9(2). https://doi.org/10.1002/fes3.196

Bereza, B. M., Gonchar, О. О., & Zarytskyi, О. М. (2016). Do pytannia fizykokhimichnoi, mikrobiolohichnoi kharakterystyky antyseptykiv deksametoksynu, dekasanu, miramistynu [To the question of physical and chemical, characteristics of antiseptics of decamethoxine®, decasan®, miramistin]. Visnyk morfolohii, 22(2), 236-239. [in Ukrainian].

Knysh, O. V., Kolpak, S. A., Pohorila, M. S., & Babych, Yе. M. (2020). Adhezyvni vlastyvosti bakterii za vplyvu bezklitynnykh ekstraktiv Bifidobacterium bifidum 1 ta Lactobacillus reuteri DSM 17938 [Adhesive properties of bacteria under the influence of bifidobacterium bifidum 1 and lactobacillus reuteri DSM 17938I cell-free extracts]. Aktualni problemy suchasnoyi medytsyny, 20(2), 129-134. [in Ukrainian]. https://doi.org/10.31718/2077-1096.20.2.129

Acquaviva, R., D'Angeli, F., Malfa, G. A., Ronsisvalle, S., Garozzo, A., Stivala, A., Ragusa, S., Nicolosi, D., Salmeri, M., & Genovese, C. (2021). Antibacterial and anti-biofilm activities of walnut pellicle extract (Juglans regia L.) against coagulase-negative staphylococci. Natural product research, 35(12), 2076-2081. https://doi.org/10.1080/14786419.2019.1650352

National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 3806, Juglone. https://pubchem.ncbi.nlm.nih.gov/compound/Juglone

Sieniawska, E., Swatko-Ossor, M., Sawicki, R., Skalicka-Woźniak, K., & Ginalska, G. (2017). Natural Terpenes Influence the Activity of Antibiotics against Isolated Mycobacterium tuberculosis. Medical principles and practice, 26(2), 108-112. https://doi.org/10.1159/000454680

Cushnie, T. P., & Lamb, A. J. (2011). Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, 38(2), 99-107. https://doi.org/10.1016/j.ijantimicag.2011.02.014

Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry, 22(1), 132-149. https://doi.org/10.2174/0929867321666140916113443

Published

2023-03-28

How to Cite

1.
Mozhaiev IV, Yevsiukova VY, Torianyk II, Minukhin VV, Polishchuck NM, Kyryk DL. Effect of walnut extract supplemented with carbon dioxide on adhesive activity of agents causing wound infection. Zaporozhye medical journal [Internet]. 2023Mar.28 [cited 2024Apr.20];25(2):158-63. Available from: http://zmj.zsmu.edu.ua/article/view/263966