THE INFLUENCE OF LEVETIRACTEAM AND DIAZEPAM ON PENICILLIN-INDUCED FOCAL EPILEPTIC ACTIVITY

Authors

  • Т. N. Muratova

DOI:

https://doi.org/10.14739/2310-1210.2014.6.35861

Keywords:

Diazepam, Penicillin G, Anticonvulsants, Epilepsy

Abstract

Aim. The power of epileptic foci and their life-span in Wistar rats (44 animals) were investigated under conditions of levetiracetam (30,0 and 130,0 mg/kg, i.p.) and diazepam (0,5 and 1,5 mg/kg, i.p.) treatment in acute experiments.

Methods and results. Penicillin – induced (15.000 IU/ml) epileptic foci in rat’s cortex were suppressed by levetiracetam (30,0 and 130 mg/kg, i.p.) with the net reduction of focal epileptic power registered in 120 (by 26,8%) and 30 min (by 48,4 %) from the moment of epileptogen application correspondently. Diazepam (0,5 and 1,5 mg/kg, i.p.) being administered at the height of focal epileptoigenesis, reduced focal activity in 15,0 and 5,0 min by 41,4 and 46,8% correspondently. In 5,0 min from the moment of diazepam administration (1,5 mg/kg, i.p.), which was made after preliminary (in 30 min) treatment with LVR (30,0 mg/kg, i.p.) was followed by 3,7 times reduction of power of focal epileptic activity, when compared with control group (P<0,05).

Conclusion. The life-span of focal activity under conditions of combined usage of LVR and diazepam  was reduced up to 110,5+11,2 min from 270,5+ 24,5 min in control group (P<0,05).

References

Zenkov, L. R. (2012) Generalizovannye e`pilepsii: sovremennye koncepcii i terapevticheskie podkhody [Generalized epilepsy: contemporary conceptions and therapeutical approaches]. Nevrologiya, nejropsikhiatriya, psikhosomatika, 1, 72–82. [in Russian].

Mazarati, A. M., Baldwin, R., Klitgaard, H., et al. (2004) Anticonvulsant effects of levetiracetam and levetiracetam-diazepam combinations in experimental status epilepticus. Epilepsy Res., 58 (2–3), 167–174.

Czapinski, P., Blaszczyk, B., & Czuczwar, S. J. (2005) Mechanisms of Action of Antiepileptic Drugs. Current Topics in Medicinal Chemistry, 5, 3–14.

De Smedt, T., Raedt, R., Vonck, K., & Boon, P. (2007) Levetiracetam: the profile of a novel anticonvulsant drug-part I: preclinical data. CNS Drug Rev., 13, 3–56.

Arık, A. E., Bağırıcı, F., Sefil, F., & Marangoz, C. (2014) Effect of levetiracetam on penicillin induced epileptic activity in rats. Acta Neurobiol. Exp, 74, 266–275.

Rigo, J. M., Hans, G., Nquyen, L., et al. (2002) The antiepileptic drug levetiracetam reverses the inhibition by negative allosteric modulators of neuronal GABA- and glycine-gated currents. Br. J. Pharmacol., 136, 659–672. doi: 10.1038/sj.bjp.0704766.

Castel-Branco, M. M., Alves, G. L., Figueiredo, I. V. et al. (2009) The maximal electroshock seizures (MES) model in the preclinical assesment of potential new antiepileptic drugs. Methods Find. Exp. Clin. Pharmacol., 31(2), 101–106. doi: 10.1358/mf.2009.31.2.1338414.

Vogl, C., Mochida, S., Wolff, C. et al. (2012) The synaptic vesicle glycoprotein 2A ligand levetiracetam inhibits presynaptic Ca2+ channels through an intracellular pathway. Mol. Pharmacol., 82, 199–208. doi: 10.1124/mol.111.076687.

Loescher, W. (2011) Critical review of current animal models of seizures and epilepsy used in the discovery and development of new antiepileptic drugs. Seizure, 20, 359–368. doi: 10.1016/j.seizure.2011.01.003.

Lukyanetz, E. A. Shkryl, V. M., & Kostyuk, P. G. (2002) Selective blockade of N-type calcium channels by levetiracetam. Epilepsia, 43, 9–18. doi: 10.1046/j.1528-1157.2002.24501.x.

Modur, P. N., Miller, W. E., & Zhang, S. (2010) Sequential intrarectal diazepam and intravenous levetiracetam in treating acute repetitive and prolonged seizures. Epilepsia, 51(6), 1078–1082. doi: 10.1111/j.1528-1167.2009.02385.x.

Nagarkatti, N., Deshpande, L. S., & DeLorenzo, R. J. (2008) Levetiracetam inhibits both ryanodine and IP3 receptor activated calcium induced calcium release in hippocampal neurons in culture. Neurosci. Lett., 436, 289–293. doi: 10.1016/j.neulet.2008.02.076.

Meador, K. J., Gevin, A., Leese, P. T., et al. (2011) Neurocognitive effects of brivaracetam, levetiracetam, and lorazepam. Epilepsia, 52(2), 264–272. doi: 10.1111/j.1528-1167.2010.02746.x.

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1.
Muratova ТN. THE INFLUENCE OF LEVETIRACTEAM AND DIAZEPAM ON PENICILLIN-INDUCED FOCAL EPILEPTIC ACTIVITY. Zaporozhye medical journal [Internet]. 2014Dec.8 [cited 2024Apr.20];16(6). Available from: http://zmj.zsmu.edu.ua/article/view/35861

Issue

No. 6 (2014): Zaporozhye medical journal

Section

Original research

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