β-alanine spectrophotometric determination in reaction with sodium salt of 1,2-naphthoquinone-4-sulfonic acid

Authors

  • K. P. Portna
  • S. O. Vasyuk

DOI:

https://doi.org/10.14739/2310-1210.2015.1.39870

Keywords:

spectrophotometry, analysis, beta-alanine, 1, 2-naphthoquinone-4-sulfonate, validation studies

Abstract

Aim. The new spectrophotometric method for the quantitative determination of β-alanine in pharmaceutical formulations has been developed.

Methods and results. This method is based on the measurement of aqueous β-alanine solutions absorption at 470 nm. The proposed method is actual according to the validation requirements of Ukrainian Pharmacopeia. The analytical method was optimized and validated by establishing the linearity (the correlation coefficient r = 1,000), precision (RSD% = 0,806, n = 9) and the accuracy ( = 100,8 %).

Conclusion. According to the experimental data, the technique can be correctly reproduced and it is suitable for using in laboratories of the State Inspection for Quality Control of Medicines and QCD of the chemical-pharmaceutical enterprises.

References

(2003). United States Pharmacopeia 26. – USP Convention Inc. Rockville.

(2000). British Pharmacopeia / Her Majesty’s Stationery Office. London.

Abe, T, Kurozumi, Y, Yao, WB, & Ubuka, T. (1998) High-performance liquid chromatographic determination of beta-alanine, beta-aminoisobutyric acid and gamma-aminobutyric acid in tissue extracts and urine of normal and (aminooxy)acetate-treated rats. Journal of Chromatography B, 712(1), 43–49. doi: 10.1016/S0378-4347(98)00154-6.

Van Kuilenburg, A. B., Stroomer, A. E., Peters, G. J., Van Gennip, A. H. (2001) Simultaneous determination of F-β-alanine and β-alanine in plasma and urine with dual-column reversed-phase high-performance liquid chromatography. Journal of Chromatography B, 759(1), 51–61. doi: 10.1016/S0378-4347(01)00203-1.

Baxter, J. H. & Johns, P. W. (2012) Determination of Free Arginine, Glutamine, and β-alanine in Nutritional Products and Dietary Supplements. Food Analytical Methods, 5(4), 821–827. doi: 10.1007/s12161-011-9318-x.

Oshkaya, V. P. (1974). Ningidrinovye reakcii [The ninhydrin reactions]. Riga: Zinatne [in Latvia].

Korzhova, A. S. (2005) Analiz likarskykh rechovyn z pervynnoiu alifatychnoiu aminohrupoiu ta solei slabkykh orhanichnykh kyslot po reaktsii z 1,3-dymetyl-aloksanom ta pokhidnymy benzoilhidrazonu-1,4-benzokhinonoksymu (Dis…kand. farm. nauk). [Drug substances analysis with primary aliphatic amino group and salts of weak organic acids in reaction with 1,3-dimethyl-alloxan and derivative of benzoilhydrazon-1,4-benzohynonoxym. Dr. farm. sci. diss.]. Zaporizhzhya. [in Ukrainian].

Bulatov, M. I., & Kalinkin, I. P. (1986) Prakticheskoe rukovodstvo po fotometricheskim metodam analiza [The practical guide to photometric methods of analysis]. Leningrad: Himiya. [in Russian].

(2008) Derzhavna Farmakopeja Ukrai'ny. Dopovnennja 2. [Ukrainian Pharmacopoeia. Addition 2]. Harkiv. [in Ukrainian].

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How to Cite

1.
Portna KP, Vasyuk SO. β-alanine spectrophotometric determination in reaction with sodium salt of 1,2-naphthoquinone-4-sulfonic acid. Zaporozhye Medical Journal [Internet]. 2015Feb.9 [cited 2024Oct.6];17(1). Available from: http://zmj.zsmu.edu.ua/article/view/39870

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Section

Problems of pharmacy