Immune status changes in patients with phlegmon in the maxillofacial region relation with purulent process prevalence
DOI:
https://doi.org/10.14739/2310-1210.2016.1.63647Keywords:
Cellulitis, Mouth, Face, Neck, Interleukins, Immune System Diseases, Prevalence, Mediastinitis, Asymptomatic DiseasesAbstract
Introduction. Significant portion of patients with acute purulent-inflammatory diseases of the face and neck often has a pronounced secondary immunodeficiency. One manifestation of violation of protective functions in this group of patients are changes in the synthesis of interleukin blood. Knowledge about the of the immune changes in the early stages of the face and neck abscesses development can be used for choosing the right timely empirical therapy. However, the currently available data regarding immune abnormalities in this pathology are controversial. Perhaps this is due to the influence of geographical, environmental and socio-economic factors on the immune status of patients and the results of studies. Thus, to obtain an objective picture of the nature of changes in the body's defenses of patients with inflammatory processes of the face and neck, it is advisable to carry out the study of the state of immunity for each region separately, and that was the basis for this study
Aim. to improve pyoinflammatory facial diseases diagnostics on the background of the cytokine status study.
Materials and Methods. Serum cytokines (IL-1, TNFα, IL-6, IL-8, IL-4) concentration in 60 patients with maxillofacial phlegmon were determined with ELISA method. All patients were residents of Zaporozhye and the Zaporozhye area. Patients were divided into III groups: the 1st included 40 (66.7%) patients with a checkered phlegmons space; 2nd group – 15 (25.0%) patients with phlegmons of the more than one space; the 3rd group – 5 (8.3%) patients with diffuse face and neck phlegmon, and with mediastinitis. The average age of patients was 37.0±7 years. 21 healthy subjects were included into control group.
5 ml of the peripheral blood was taken from the antecubital vein under aseptic conditions; and was placed in sterile tubes containing 25 IU of heparin per 1ml. Blood was centrifuged at 3000 rev./min for 10 minutes. Serum was dispensed at 0.3 ml. in plastic tubes, frozen and stored before use at minus 20°C. cytokines concentration was determined with commercial test systems OOO "cytokine" "Vector-Best" (St. Petersburg). The results were recorded according to absorbance measured in a spectrophotometer – analyzer Sunrise (Austria) at 450 nm. The cytokines were determined by constructing a calibration curve using the computer program «Calibration» in picogram per milliliter (pg/ml) units.
The results and discussion. All phlegmons of the maxillofacial region, regardless of their degree of prevalence are accompanied by an increase in the blood levels of pro-inflammatory interleukins majority. IL-4 concentration in the blood of patients with phlegmones was close to normal range (0.43±0.03, р>0.5). Serum IL4 concentration was increased only in patient with pyoinflammatory changes in more than one area. This indicates adequate response of the immune system against massive antigen load of the immune system (0.93±0.05, p<0.001). IL 4 serum levels in patient with mediastinitis were lower than in the control group (0.25±0.03, p<0.01). this indicated immunosupression in such cases.
References
Barannik, N. G. , Varzhapetyan, S. D., Kuropata, I. V., Berdyuk, I. V., & Manukhina, O. N. (2014) Izuchenie faktorov, vliyayuschikh na rasprostranennost′ ostrogo odontogennogo osteomielita sredi zhitelej promyishlennogo goroda Zaporozh′ya [The study of factors affecting the prevalence of acute odontogenic osteomyelitis among the residents of the industrial city of Zaporizhia]. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 2, 81–84. [in Ukrainian].
Barannik, N. G., & Varzhapetyan, S. D. (2015) Sostoyanie citokinovogo statusa bol′nykh vyalotekuschim ostrym odontogennym osteomielitom chelyustej [Status cytokine status of patients with indolent acute odontogenic osteomyelitis of the jaws]. Scienc Rise, 1, 3(6), 25–29. [in Ukrainian].
Porfiriadis, M. P., Shulakov, V. V., Sashkina, T. I., & Volozhin, A. I. (2010) Povyshenie e′ffektivnosti lecheniya vyalotekuschikh flegmon chelyustno-litsevoj oblasti s pomoschyu immunomodulyatora Poliksidoniya [Increase of the treatment efficacy of the slow developing phlegmonas of maxillofacial region with the help immunomodulator Polyoxidonium]. Stomatologiya, 4, 47–49. [in Russian].
Ivaniushko, T. P., Gankovskaia, L. V., Shamanaev, S. V., Svitich, O. A., Kartashov, D. D., Grechenko, V. V., & Balykin, R. A. (2014) Izuchenie soderzhaniya defenzimov u bol′nykh s gnojno-vospalitel′nymi zabolevaniyami chelyustno-licevoj oblasti [Defensines level in patients with inflammatory diseases of maxillofacial area]. Stomatologiya, 93(2), 23–26. [in Russian].
Dobrov, A. V., Cidorov, I. A., Cidorova, O. A., Belopukhov, V. M., & Utkelbaev, R. I. (2012) Immunologicheskie osobennosti u bol′nykh s tyazhelymi gnojno-vospalitel′nymi zabolevaniyami lica i shei, oslozhnennykh vtorichnym gnojnym mediastinitom [Immunological peculiarities of patietnts with heavy face and neck pyoinflammatory diseases which are complicated by secondary pyogenic mediastinitis]. Sovremennye problemy nauki i obrazovaniya, 3, 67. [in Russian].
Karpin, V. A., & Filatova, O. E. (2012) Bioinformacionnyj analiz vliyaniya geliogeomagnitnoj aktivnosti na sostoyanie zaschitnykh sil organizma cheloveka v e′kologicheskikh usloviyakh vysokikh shirot [Bioinformation analysis of the heliomagnetic activity influence on the human organism protective strength in the high latitude ecological condition]. Fundamental′nye issledovaniya, 9–3, 563–567. [in Russian].
Kovaleva, N. S., & Zuzova, A. P. (2011) Gnojno-vospalitel′nye zabolevaniya chelyustno-licevoj oblasti: aspekty kliniki, mikrobiologii, farmakoterapii [Pyoinflammatory diseases of the maxillofacial area: aspects of clinic, microbiology, and pharmacotherapy]. Farmateka, 18, 34–38. [in Russian].
Shikhanyan, N. N., Turovaya, A. Yu., Kade, A. Kh., Mosesova, A. S., & Uvarov, A. V. (2014) Primenenie TES-terapii v kompleksnom lechenii odontogennyih flegmon chelyustno- A. V. litsevoy oblasti [Applications TES-therapy in treatment of odontogenic abscesses maxillofacial area]. Fundamental′nye issledovaniya, 1, 87–88. [in Russian].
Pradhan, A. D., Manson, J. E., Rifai, N., Buring, J. E., & Ridker, P. M. (2001) C-reactive protein, interleukin 6, and the risk of developing type 2 diabetes mellitus. JAMA, 286, 327–334.
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