Bioelectrical activity of interneyron pools of spinal cord an early stage of experimental compressive neuropathy and prednisolone effects
Keywords:Interneurons, Spinal Cord, Compression Neuropathy, Hyperreflexia, Prednisolone
In experiments on white rats examined the activityof interneyron pools of spinal cord (SC) in the early stages (from the 10th day) of compression neuropathy (CN) of the sciatic nerve.
Aim. To study the effect of prednisolone on the activity of the spinal cord interneuron pools in the early stages (up to the 10th day) of compression neuropathy of the sciatic nerve.
Materials and methods. The animals were divided into two groups: «intact animal» (n = 10) and «animals with simulated state» (n = 30). Every animal in the group «simulated state» has daily intraperitoneal injectson of Prednisolon solution at a dose of 5 mg / kg.
In our investigation we used the method of recording of potential dorsal surface (PDS) of the SC. PDS as integral indicators characterizing the activity of interneurons of SC, which occurs in response to stimulation of afferent fibers.
We investigated the following parameters of PDS: threshold, chronaxi, duration of the latent period , the duration of afferents peak, duration N1, N2, N3 components and P-waves, investigated a total duration of PDS and amplitude of afferents peak and N1, N2, N3 components and P-waves respectively.
Results. The experimental introduction on the background CN prednisolone causes changes in the activity of neurons posterior horns of the SC, which manifests itself in increasing the threshold occurrence of PDS. Changes of chronaxi of PDS - extension to 110,0 ± 10,50 ms (n = 6, p <0.01), showed significant changes biophysical characteristics of Na+ -channels, and require more detailed study.
Conclusions. Perhaps these changes suggest that there is a strengthening inhibitory effect on afferent nerve fibers or decrease the expression of ion channels are results of catabolic action of prednisolone.
Akhmedova, G. M., & Zimakova, T. V. (2012) Podgrushevidnaya sedalishhnaya nejropatiya: klinicheskie varianty i algoritm terapii [Subpiriforme sciatic neuropathy: clinical variants and therapy algorithm]. Praktitscheckaja medizina, 2(57), 129–131. [in Russian].
Babichev, V. N. (2005) Nejroe'ndokrinnyj e'ffekt polovykh gormonov [Neuroendocrine Effect Sex Hormones]. Ucpechi visiologitscheckich nauk, 36(1), 54–67. [in Russian].
Golikov, P. P. (1988) Receptornye mekhanizmy glyukokortikoidnogo e'ffekta [Receptor mechanisms of the glucocorticoid effects]. Moscow: Medicine. [in Russian].
Makij, E. А., Nerush, P. А., & Rodinskij, А. G. (2001) Parametry potenciala dorsal'noj poverkhnosti spinnogo mozga krys pri e'ksperimental'nom gipertireoze [Parameters of the potential of the dorsal surface of the spinal cord of rats with experimental hyperthyroidism]. Nejrofiziologiya, 4, 279–285. [in Ukrainian].
Makij, E. А., Rodinskij, А. G, Gninenko, A. N., & Belokon', V. N. (2007) Dinamika postdenervacionnykh izmenenij spinal'noj reflektornoj aktivnosti u belykh krys [Dynamics of changes in spinal reflex activity of white rats]. Nejrofiziologiya, 39(1), 37–46. [in Ukrainian].
Platonov, А. E. (2000) Statisticheskij analiz v medicine i biologii: zadachi, terminologiya, logika, komp'yuternye metody [Statistical analysis in medicine and biology tasks, terminology, logic, computer techniques] Moscow. [in Russian].
Fomіn, G. M., Tsymbaliuk, V. І., & Sapon, M. А. (1999) Ushkodzhennia sіdnychnogo nerva, zumovlenі perelomamy kіstok taza і stehna [Damage to the sciatic nerve caused by fractures of the pelvis and hips]. Shpytalna khirurhiia, 2, 62–65. [in Ukrainian].
Shuhurov, O. O. (1999) Osoblyvosti vidvedennia masovykh spynnomozkovykh potentsialiv poza spynnym mozkom [Features diversion mass potential outside the spinal cord dorsal]. Nejrovisiologija, 2, 120–122. [in Ukrainian].
Andó, R. D., & Sperlágh, B. (2013) The role of glutamate release mediated by extrasynaptic P2X7 receptors in animal models of neuropathic pain. Brain Research Bulletin, 93, 80–85. doi: 10.1016/j.brainresbull.2012.09.016
Blizzard, C. A., King, A. E., Haas, M. A., O'Toole, D. A., Vickers, J. C., & Dickson, T. C. (2009) Axonal shearing in mature cortical neurons induces attempted regeneration and the reestablishment of neurite polarity. Brain Res, Dec, 1, 1300, 24–36. doi: 10.1016/j.brainres.2009.08.059
Bronfman, F. C., Lazo, O. M., Flores, C., & Escudero, C. A. (2014) Spatiotemporal intracellular dynamics of neurotrophin and its receptors. Implications for neurotrophin signaling and neuronal function. Handb Exp Pharmacol, 220, 33–65. doi: 10.1007/978-3-642-45106-5_3.
Cao, J., Cai, F.C., Chen, J., & Zhang, X. P. (2010) Experimental study on the damage of immature brain induced by chronic treatment with exogenous glucocorticosteroid. Chinese Journal of Pediatrics, 48(2), 131–137.
Cao J., Wang, J. S., Ren, X. H., & Zang, W. D. (2015) Spinal sample showing p-JNK and P38 associated with the pain signaling transduction of glial cell in neuropathic pain. Spinal Cord, 53(2), 92–7. doi: 10.1038/sc.2014.188.
Chen, Y. C., Pristerá, A., Ayub, M., Swanwick, R. S., Karu, K., Hamada, Y., et al. (2013) Identification of a receptor for neuropeptide VGF and its role in neuropathic pain. Biological Chemistry, 29, 38–46. doi: 10.1074/jbc.M113.510917.
D’Mello, R., & Dickenson, A. H. (2008) Spinal cord mechanisms of pain. British Journal of Anaesthesia, 101(1), 8–16. doi: 10.1093/bja/aen088.
Decosterd, I., & Woolf, C. J. (2000) Spared nerve injury: an animal model of persistent peripheral neuropathic pain. Pain Med, 87(2), 149–158. doi: 10.1016/S0304-3959(00)00276-1.
Pitcher, G. M., Ritchie, J., & Henry, J. L. (2013) Peripheral neuropathy induces cutaneous hypersensitivity in chronically spinalized rats. Pain Med. 14(7), 1057–71. doi: 10.1111/pme.12123.
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