Severity of endogenous intoxication in tuberculosis/HIV co-infected patients with first diagnosed pulmonary tuberculosis
DOI:
https://doi.org/10.14739/2310-1210.2016.4.79692Keywords:
Tuberculosis, HIV, EndotoxinsAbstract
Biochemical parameters participation in systemic inflammatory response syndrome (SIRS) development at tuberculosis/HIV co-infection has not been carefully studied by now and severity of endogenous intoxication, as well as its influence on the disease occur.
Aim. To determine the intensity of endogenous intoxication in tuberculosis/HIV co-infected patients with first diagnosed pulmonary tuberculosis (FDTB/HIV), depending on the severity of the disease.
Materials and methods. 54 patients with FDTB/HIV were examined and divided into 3 groups. The 1st group included 15 patients without SIRS, with local tuberculosis, without massive bacterial excretion, with the number of CD4+-cells more than 200 in 1 ml, or if there was the only one of the next signs (disseminative destructive tuberculosis, massive bacterial excretion, the number of CD4+-cells less than 200 in 1 ml). The 2nd group included 13 patients who had more than one sign. The 3rd group consisted of 26 patients with SIRS. SIRS was diagnosed by R. Bone et al. (1992). As a markers of endogenous intoxication C-reactive protein (CRP), rheumatoid factor (RF), fibrin, seromucoid by standard methods; α1-antitrypsin (α1-AT) – by immunoassay kit Immundiagnostik, Germany; protein fractions were determined by electrophoresis; intermediate mass molecules (IMM) – by B. Halliwell method (1999) were measured.
Results. In FDTB/HIV patients seromucoid, fibrin, α1-AT, γ-globulins and IMM levels were increased; albumin levels and albumin-globulin ratio were reduced independently of the process severity. The levels of CRP and RF were also increased if disease was severer. Dysproteinemia is compounded in the presence of SIRS and IMM levels 2-fold increase compared to control.
Conclusion. This indicates that the levels of acute inflammation phase marks, protein fractions and IMM are dependent not only on the presence of pathogens in the organism, but also on the disease severity.
References
Anisimova, T. M., & Vysokogorsky, V. E. (2010). Vliyanie preparata Glutoksim na processy svobodnoradikal'nogo okisleniya i sostoyanie sistemy glutationa pri kompleksnoj terapii odontogennykh flegmon [Influence of preparation glutoksim on processes свободнорадикального of oxidation and system glutationa condition at complex therapy odontogenic of phlegmons]. Medicinskaya nauka i obrazovanie Urala, 2, 85–87. [in Russian].
Borodin, E. A., Egorshina, E. V., & Samsonov, V. P. (2003). Biokhimiya e´ndotoksikoza. Mekhanizmy razvitiya i ocenka stepeni tyazhesti pri vospalitel´nykh zabolevaniyakh legkikh [Biochemistry of endotoxemia. Pathogenic mechanisms and the estimation of the degree of severity at the inflammatory lung diseases]. Blagoveshchensk: AGMA. [in Russian].
Piddubna, A. I., & Chemych, M. D. (2009). VIL-infektsiia: kliniko-imunolohichni aspekty [HIV infection: clinical and immunological aspects]. Visnyk SumDU. Seriia Medytsyna, 1, 160–168. [in Ukrainian].
Ryazanceva, L. T. (2011). Fermenty-antioksidanty: strukturno-funkcional'nye svojstva i rol' v regulirovanii metabolicheskikh processov [Antioxidant enzymes: structural and functional properties and role in the regulation of metabolic processes]. Vestnik Voronezhskogo gosudarstvennogo tehnicheskogo universiteta, 7(2), 126–129. [in Russian].
Kaminskaja, G. O., Abdullaev, R. Yu., Martynova, E. V., Serebryanaya, B. A., & Komissarova, O. G. (2009). Sindrom sistemnogo vospalitel'nogo otveta pri tuberkuleze legkikh [Systemic inflammatory response syndrome at a pulmonary tuberculosis]. Problemyi tuberkuleza i boleznej legkikh, 11, 40–48. [in Russian].
Danilova, L. A. (2003). Spravochnik po laboratornym metodam issledovaniya [Handbook of Laboratory Methods]. Saint Petersburg: Piter. [in Russian].
Titov, V. N. (2008). S-reaktivnyj belok – vektor perenosa zhirnykh kislot k kletkam, kotorye neposredstvenno realizuyut sindrom sistemnogo vospalitel'nogo otveta [C-reactive protein – fatty acid transfer vector to cells that directly implement the systemic inflammatory response syndrome]. Klinicheskaya laboratornaya diagnostika, 6, 3–13. [in Russian].
Mitinskaya, L. A., Elufimova, V. F., Jukhimenko, N. V., Kaminskyja, G. O., & Abdullaev, R. Yu. (2004). Fenotipy gaptoglobina i uroven' ostrofaznykh belkov v syvorotke krovi u detej s lokal'nymi formami vnutrigrudnogo tuberkuleza [Haptoglobin phenotypes and the level of serum acute phase proteins in children with intrathoracic TB local forms]. Problemy tuberkuleza i boleznjy legkikh, 7, 35–39. [in Russian].
Chernushenko, E. F., & Protsiuk, R. G. (2011). Protivotuberkuleznyj immunitet. Chast' 2 [TB immunity. Part 2]. Ukrainskyi pulmonolohichnyi zhurnal, 1, 29–32. [in Ukrainian].
Davalos, D., & Akassoglou, K. (2012). Fibrinogen as a key regulator of inflammation in disease. Seminars in immunopathology, 34(1), 43–62. doi: 10.1007/s00281-011-0290-8.
Drain, P. K., Mayeza, L., Bartman, P., Hurtado, R., Moodley, P., Varghese, S., et al. (2014). Diagnostic accuracy and clinical role of rapid C-reactive protein testing in HIV-infected individuals with presumed tuberculosis in South Africa. The international journal of tuberculosis and lung disease, 18(1), 20–26. doi: 10.5588/ijtld.13.0519.
Suresh, D. R., Annam, V., Pratibha, K., & Hamsaveena. (2010). Immunological correlation of oxidative stress markers in tuberculosis patients. International journal of biological and medical research, 1(4), 185–187.
Robertson, C. M., & Coopersmith, C. M. (2006). The systemic inflammatory response syndrome. Microbes and Infection, 8(5), 1382–1389.
Serapinas, D. (2012). Alpha-1 antitrypsin, inflammation and quality of life. Biologija, 58 (2), 79–86.
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