Comparative immunohistochemical characteristics of Ki-67, p53, caspase-3 expression in gastric hyperplastic polyps and non-invasive intestinal-type gastric adenocarcinoma
DOI:
https://doi.org/10.14739/2310-1210.2016.4.79773Keywords:
polyps, gastric cancer, cell proliferation, apoptosisAbstract
Aim. The problem of early diagnostic of gastric cancer has a tight connection with the issue of premalignant lesions, especially gastric polyps. The aim of this research was to compare the expression levels of Ki-67, p53 and caspase-3 in epithelial cells and stromal cells of gastric hyperplastic polyps on the one hand and tumor cells and stromal cells of non-invasive intestinal-type gastric adenocarcinoma on the other hand.
Materials and methods. For this purpose, pathohistological and immunohistochemical studies were used. The biopsies of 10 patients with hyperplastic polyps (the age of the patients ranged from 52 to 73 years) and surgical material of 10 patients with non-invasive (pTis, pT1) intestinal-type gastric adenocarcinoma (the age of patients ranged from 45 to 83 years) were analyzed.
Results. It was established that epithelial cells of gastric hyperplastic polyps are characterized by low expression level of Ki-67, p53 and caspase-3, just as stromal cells of hyperplastic polyps are characterized by low expression level of Ki-67 and the medium expression level of caspase-3. Moreover, it has been established that tumor cells of non-invasive intestinal-type gastric adenocarcinoma are characterized by medium expression level of Ki-67, caspase-3 and also low expression level of p53, just as stromal cells of non-invasive adenocarcinoma are characterized by low expression level of Ki-67 and low expression level of caspase-3. The differences between the expression levels of Ki-67, p53 and caspase-3, that were established for hyperplastic polyps on the one hand and non-invasive adenocarcinoma on the other hand, are statistically significant (p<0,05).
Conclusion. These data demonstrate, that proliferative activity of non-invasive intestinal-type gastric adenocarcinoma tumor cells is higher than proliferative activity of gastric hyperplastic polyps epithelial cells. Moreover, non-invasive intestinal-type gastric adenocarcinoma differs by higher activity of p53 accumulation, higher level of tumor cells apoptosis and lower level of stromal cells apoptosis.
References
Jemal, A., Siegel, R., & Torre, L. (2015) Global Cancer Facts & Figures. Atlanta: American Cancer Society.
Fedorenko, Z. P., Gulak, L. O., Mykhailovych, Yu. Y., Horokh, E. L., Ryzhov, A. U., Sumkina, O. V., & Kutsenko, L.B. (2016). Rak v Ukraine, 2014–2015 [Cancer in Ukraine, 2014–2015]. Biulleten nacional'noho kantser-reiestru Ukrainy, 17, 37. [in Ukrainian].
Barannikov, K. V., Shvydkyi, Ya. B., Tumak, I. M., & Lukavetskyi, N. O. (2013) Polipy shlunka: suchasna klasyfikatsiia, endoskopichna diahnostyka, vybir taktyky [Gastric polyps: current classification, endoscopic diagnosis, choice of tactics]. Lvivskyi medychnyi chasopys, 19(4), 67–75. [in Ukrainian].
Tumanskij, V. A., & Zubko, M. D. (2015) E´kspressiya markerov progressirovaniya opukholi v gepato- i kholangiotsellyulyarnom rake pecheni raznoj velichiny [The expression of tumor progression markers in hepatocellular and cholangiocellular liver carcinomas of different sizes]. Aktualni pytannia farmatsevtychnoi i medychnoi nauky ta praktyky, 2(18), 93–97. [in Ukrainian]. DOI: http://dx.doi.org/10.14739/2409-2932.2015.2.46615
Tumanskij, V. A., & Chepec, A. V. (2016) Sravnitel´naya immunogistokhimicheskaya kharakteristika e´kspressii receptorov e´strogena-α i progesterona, p16 i p53, Ki-67 i kaspazy-3 v invazivnoj e´ndometrioidnoj adenokarcinome tela matki i v proliferativnom e´ndometrii [Comparative immunohistochemical characteristics of expression of estrogen receptors-alpha, progesterone receptors, p16, p53, Ki-67 and caspase 3 in invasive endometrial adenocarcinoma and normal proliferative endometrium]. Pathologia, 1(36), 22–28. [in Ukrainian].DOI: http://dx.doi.org/10.14739/2310-1237.2016.1.72165
Tumanskyi, V. O., Baudarbekova, M. M., & Chepets, O. V. (2014) Osoblyvosti protsesiv proliferatsii ta apoptozu v neinvazyvnii adenokartsynomi endometriiu ta adenokartsynomi z invaziieiu v miometrii [Specific features of proliferation and apoptosis in non-invasive adenocarcinoma of endometrium and adenocarcinoma with invasion into myometrium]. Pathologia, 1(30), 63–67. [in Ukrainian]. DOI: http://dx.doi.org/10.14739/2310-1237.2014.1.25957
Engels, C. C., Ruberta, F., Kruijf, E. M., Pelt, G. W., Smit, V. T., Liefers, G. J., et al. (2013) The prognostic value of apoptotic and proliferative markers in breast cancer. Breast Cancer Research and Treatment, 2(142), 323–39. doi: 10.1007/s10549-013-2748-y.
Bascones-Martínez, A., Rodríguez-Gutierrez, C., Rodríguez-Gómez, E., Gil-Montoya, J.A., Gómez-Font, R., & González-Moles, M. Á. (2013) Evaluation of p53, caspase-3, Bcl-2, and Ki-67 markers in oral squamous cell carcinoma and premalignant epithelium in a sample from Alava Province (Spain). Medicina Oral Patologia Oral Y Cirugia Bucal, 18(6), 846–50. doi: 10.4317/medoral.18901.
Yao, T., Kajiwara, M., Kuroiwa, S., Iwashita, A., Oya, M., Kabashima, A., & Tsuneyoshi, M. (2002) Malignant transformation of gastric hyperplastic polyps: Alteration of phenotypes, proliferative activity, and p53 expression. Human Pathology, 33(10), 1016–22.
Tumanskij, V. A., & Khrystenko, T. A. (2014) Giperplasticheskie polipy zheludka: immunogistokhimicheskaya kharakteristika proliferacii i apoptoza [Gastric hyperplastic polyps: immunohistochemical characteristic of proliferation and apoptosis]. Patolohiia, 3(32), 96–99. [in Ukrainian].DOI: http://dx.doi.org/10.14739/2310-1237.2014.3.37045
Murakami, K., Mitomi, H., Yamashita, K., Tanabe, S., Saigenji, K., & Okayasu, I. (2001) P 53, but not c-Ki-ras, mutation and down-regulation of p 21 WAF1/CIP1 and cyclin D1 are associated with malignant transformation in gastric hyperplastic polyps. American Journal of Clinical Pathology, (115)2, 224–34. doi: 10.1309/VLF5-UCNH-XQM2-X410. •
Joo, Y. E., Chung, I. J., Park, Y. K., Koh, Y. S., Lee, J. H., Park, C. H., et al. (2006) Expression of Cyclooxygenase-2, p53 and Ki-67 in Gastric Cancer. Journal of Korean Medical Science, 21(5), 871–76. doi: 10.3346/jkms.2006.21.5.871.
Uhlén, M., Fagerberg, L., Hallström, B., Lindskog, C., Oksvold, P., Mardinoglu, A., Sivertsson, A., Kampf, C., Sjöstedt, E., Asplund, A., Olsson, I., Edlund, K., Lundberg, E., Navani, S., Szigyarto, C., Odeberg, J., Djureinovic, D., Takanen, O., J. (2015) Tissue-based map of the human proteome. Science, 6220(347), 563–72. doi: 10.1126/science.1260419.
Tumanskyi, V. A., Yevsieiev, A. V., Kovalenko, I.S., & Zubko, M. D. (patentee) (2015) Patent 99314 Ukrainy МПК 2015.01 G01N 21/00 G06K 9/00 Sposib fototsyfrovoii morfometrii imunohistokhimichnykh preparativ [Patent of Ukraine 99314 МПК 2015.01 G01N 21/00 G06K 9/00 The technique of digital morphometry of immunohistochemical slides]. Biulleten, 10. [in Ukrainian].
Johji, I., Shinichi, H., Kazuhito, I., Shigeharu, M., Takahiko, N., Kazuhiro, N., et al. (2014) Malignant transformation of hyperplastic gastric polyps: An immunohistochemical and pathological study of the changes of neoplastic phenotype. Oncology letters, 7(5), 1459–63. doi: 10.3892/ol.2014.1932.
Shomori, K., Nishihara, K., Tamura, T., Tatebe, S., Horie, Y., Nosaka, K., et al. (2010) Geminin, Ki67, and minichromosome maintenance 2 in gastric hyperplastic polyps, adenomas, and intestinal-type carcinomas: pathobiological significance. Gastric Cancer, 13, 177–85. doi: 10.1007/s10120-010-0558-z.
Tiwari, E., Pallipady, A., Misra, R., & Mishra, S. (2015) P53 and ki67 Immunostaining in Gastric Biopsies: A Histopathological Study. International Journal of Scientific Study, 11(2), 96–101. doi: 10.17354/ijss/2015/60.
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