Assessment of leptin gene (Arg223Gln) genotypes polymorphism in patients with coronary heart disease and obesity

Authors

  • P. G. Kravchun Kharkiv National Medical University,
  • O. I. Kadykova Kharkiv National Medical University,
  • N. G. Ryndina Kharkiv National Medical University,
  • S. O. Krapivko Kharkiv National Medical University,
  • R. V. Pashtiani Kharkiv National Medical University,

DOI:

https://doi.org/10.14739/2310-1210.2017.2.95611

Keywords:

genetic polymorphism, leptin, coronary heart disease, obesity

Abstract

Aim of study – to estimate leptin gene (Arg223Gln) genotypes polymorphism in patients with coronary heart disease and obesity.

Materials and Methods. 222 patients with the coronary heart disease (CHD) and obesity have been comprehensively examined. All patients were on treatment in cardiologic unit of KUZ of the Kharkiv city hospital № 27, which is the basic clinic of Department of Internal Medicine № 2 and Clinical Immunology and Allergology of the Kharkiv National Medical University of MH of Ukraine. The comparison group consisted of 115 ischemic heart disease patients with normal body weight. 35 practically healthy persons formed the control group. Groups were comparable in age and sex. The study didn't include the patients with the serious accompanying pathology of respiratory organs, digestion, kidneys and persons with oncological diseases.

Results. In the control group there was the following distribution of leptin gene (Arg223Gln) alleles and genotypes polymorphism frequency: 15 people were allele A that made 42.86 %, allele G – 20 people (57.14 %); genotypes G/G, A/A and G/G had 15 (42.86 %), 7 (20 %) and 13 (37.14 %) persons respectively. 42 patients with CHD were allele A, which made 36.52 %, allele G – 73 patients (63.48 %). Genotypes G/G, A/A and G/G had 47 (40.87 %), 20 (17.39 %) and 48 (41.74%) patients with CHD respectively. In group of patients with combination of CHD and obesity 71 patients (31.98 %) were allele A, allele G – 151 people (68.02 %); genotypes G/G, A/A and G/G – 81 (36.49 %), 33 (14.87 %) and 108 (48.64 %) respectively. Comparison of leptin gene (Arg223Gln) alleles and genotypes polymorphism frequency of detection between groups showed the reliable differences of alleles A, G and a genotype G/G. In patients with CHD and obesity allele G was 10.88 % more and genotype G/G was 11.5 % more, than in the control group. Allele A was 10.88 % more in the control group, than in patients with CHD and obesity (p < 0.05). Reliable differences between group of CHD patients and the control group in distribution of detected alleles and genotypes frequency of leptin gene (Arg223Gln) polymorphism were not found (p < 0.05).

Conclusions. By results of the study allele G and a genotype G/G of leptin gene (Arg223Gln) polymorphism in patients with CHD has been associated with obesity.

References

Serkova, V. K., Kobrinchuk, Yu. L., & Romanova, V. А. (2011) Leptin u bol'nykh ishemicheskoj bolezn'yu serdca v sochetanii s sakharnym diabetom [Leptin in patients with coronary heart disease in combination with diabetes mellitus]. Ukrainskyi kardiolohichnyi zhurnal, 3, 19–23. [in Ukrainian].

Karbowska, J., & Kochan, Z. (2012) Leptin as a mediator between obesity and cardiac dysfunction. Postepy Hig Med Dosw, 66, 267–274. doi: 10.5604/17322693.997817.

Yiannakouris, N., Yannakoulia, M., Melistas, L., Chan, J. L., Klimis-Zacas, D., & Mantzoros, C. S. (2001) The Q223R polymor-phism of the leptin receptor gene is significantly associated with obesity and pre-dicts a small percentage of body weight and body composition variability. J. Clin. Endocrinol. Metab, 86(9), 4434–4439. doi: 10.1210/jcem.86.9.7842.

Ragin, C. C., Dallal, C., Okobia, M., Modugno, F., Chen, J., Garte, S., & Taioli, E. (2009) Leptin levels and leptin receptor polymorphism frequency in healthy populations. Infect Agent Cancer, 10(4), 1, S13. doi: 10.1186/1750-9378-4-S1-S13.

National Center for Biotechnology Information, dbSNP database. Retrieved from http://www.ncbi.nlm.nih.gov/SNP/.

Matsuoka, N., Ogawa, Y., Hosoda, K., Matsuda, J., Masuzaki, H., Miyawaki, T., et al. (1997). Human leptin receptor gene in obese Japanese subjects: evidence against either obesity-causing mutations or as-sociation of sequence variants with obesity. Diabetologia, 40(10), 1204–1210. doi: 10.1007/s001250050808.

van Rossum, C. T., Hoebee, B., Seidell, J. C., Bouchard, C., van Baak, M. A., de Groot, C. P., et al. (2002) Genetic factors as predic-tors of weight gain in young adult Dutch men and women. Int. J. Obes. Relat. Metab. Disord, 26(4), 517–528. doi: 10.1038/sj.ijo.0801964.

Quinton, N. D., Lee, A. J., Ross, R. J., Eastell, R., & Blakemore, A. I. (2001) A single nucleotide polymorphism (SNP) in the leptin receptor is associated with BMI, fat mass and leptin levels in postmenopausal Caucasian women. Hum. Genet., 108(3), 233–236. doi: 10.1007/s004390100468.

Constantin, A., Costache, G., Sima, A. V., Glavce, C. S., Vladica, M., & Popov, D. L. (2010) Leptin G-2548A and leptin receptor Q223R gene polymorphisms are not associated with obesity in Romanian subjects. Biochem Biophys Res Commun, 391(1), 282–286. doi: 10.1016/j.bbrc.2009.11.050.

Rustemoglu, A., Sahin, S., Tasliyurt, T., Kutluturk, F., & Guven, H. (2012) Relationship between obesity and Leptin (G-2548A) and Leptin receptor (668A>G (Q223R)) gene polymorphisms in Turkish population. Endocrine Abstracts, 29, 1273.

How to Cite

1.
Kravchun PG, Kadykova OI, Ryndina NG, Krapivko SO, Pashtiani RV. Assessment of leptin gene (Arg223Gln) genotypes polymorphism in patients with coronary heart disease and obesity. Zaporozhye Medical Journal [Internet]. 2017Mar.14 [cited 2024Dec.23];19(2). Available from: http://zmj.zsmu.edu.ua/article/view/95611

Issue

Section

Original research