Підбір оптимальних умов аналізу штучної суміші ізоніазиду та тіотріазоліну методом високоефективної рідинної хроматографії

L. I. Kucherenko; O. V. Khromylyova; Z. B. Moryak; H. I. Tkachenko

doi:10.14739/2310-1210.2014.2.25524

Authors

L. I. Kucherenko
O. V. Khromylyova
Z. B. Moryak
H. I. Tkachenko

DOI:

https://doi.org/10.14739/2310-1210.2014.2.25524

Keywords:

Thiotriazolin, Isoniazid, mixture, chromatography

Abstract

At present a great attention is paid to the novel physicochemical methods of drugs analysis, notably high-performance liquid chromatography. Our investigation resulted in the development of simultaneous detection of Isoniazid and Thiotriazolin content in artificial mixture by high-performance liquid chromatography method, notably choice of mobile and immobile phase. It is not easy because both components have different acid-base nature: Thiotriazolin has acid character and Isoniazid is rather strong base. Besides, during our research we chose optimal conditions of analysis: column, eluent, rate of mobile phase, analytical wavelength of detector and volume of input probe. Received chromatograms satisfy all requirements of standards and will be used for the development of standardization methods for «IT» tablets.

References

Georgievskii G.V. The development of the complex of physicochemical techniques providing creation and quality control of original domestic drugs, derivatives of 1,2,4-triazole. Zaporozhskii meditsynskii zhurnal 2011; 1(13): 58-69.

Derzhavna Farmakopeia Ukrainy[State Pharmacopeia of Ukraine]. State enterprise “Scientific and Expert Pharmacopoeial Center”. Kharkov, RIREG, 2004. 520 p.

MazurI.A.,VoloshynN.A., ChekmanI.S. Tiotriazolin[Thiotriazolin]. Zaporozhye, Lvov, Nautilus, 2005. 156 p.

Noreiko B.V. Chemotherapy of tuberculosis. Novosti meditsyny I farmatsii 2008; 19: 261.

SaprykinL.V. Praktika I metodicheskie osnovy vysokoeffektivnoi zhidkostnoi khromatografii [Practice and methodological grounds of high-performance liquid chromatography]. Krasnodar, 2006. 151 p.

ShatsV.D. Vysokoeffektivnaia zhidkostnaia khromatografiia[High-performance liquid chromatography]. Riga, Zinatne, 1988. 390 p.

FeshchenkoYu.I. Tuberculosis situation in Ukraine. Doktor 2002; 4:11-14.

Adamovics J.A. Chromatographic analysis of pharmaceuticals. - New York: Marsel Dekker, 1997.

Global tuberculosis control: WHO Report 2002. – Geneva, 2002. – 295 p.

Tuberculosis recurrences. Reinfectionplays a rolein a population whose clinical/epidemiological characteristics do not favor reinfection / deViedma D.G., Marin M., Hernangomez S. etal.// Arch. Intern. Med. – 2002. – Vol. 162, № 16. – P. 1873 – 1879.