Assessment of alcohol odor intensity as an indicator of ethanol concentration in cadavers without signs of decomposition: a correlational analysis
DOI:
https://doi.org/10.14739/2310-1210.2026.2.345990Keywords:
forensic medical examination, forensic toxicology, alcohol odour, ethanol concentration, odour intensity, correlational analysisAbstract
Aim of the study. To evaluate the reliability of assessing characteristic odor intensity, specifically alcohol, during forensic medical examinations of cadavers without signs of decomposition.
Materials and methods. A total of 43 cadavers of various ages and sexes were examined at the Department of Forensic Medical Examination of the State Specialized Institution “Kharkiv Regional Bureau of Forensic Medical Examination” between December 2024 and March 2025. Inclusion criteria: the presence of a perceptible alcohol odor, the absence of putrefactive changes, and a detectable concentration of ethyl alcohol in the blood or urine confirmed by gas chromatography. A preliminary blind test involving 12 participants was conducted to validate the observers’ ability to recognize odors from alcoholic beverages of different concentrations. During autopsy, an empirical conditional scale (ranging from 0 ‰ to 5 ‰) was utilized to quantify odor intensity. Statistical analysis was performed using Microsoft Excel and included Pearson (r) and Spearman (ρ) correlation coefficients, with significance testing performed using the χ2 test and p-values (p < 0.05). The null hypothesis assumed no significant correlation between objective ethanol concentration and perceived odor intensity.
Results. In the overall sample, a weak positive correlation was identified between odor intensity and blood alcohol concentration (r = 0.195, ρ = 0.215). Subgroup analysis based on odor localization revealed correlations ranging from moderate negative (abdominal cavity) to moderate positive (thoracic cavity, cranial cavity, and combined localizations). Analysis of urine ethanol levels showed a consistent pattern: a weak positive correlation in the general cohort and moderate positive or negative correlations depending on the site of odor assessment. Statistically significant results (p < 0.05) were predominantly observed in larger subgroups.
Conclusions. The identified correlations suggest that alcohol odor intensity serves only as a subjective and approximate indicator. It cannot replace the quantitative laboratory determination of ethanol in biological fluids due to the significant number of confounding factors influencing olfactory perception. These findings underscore the necessity for further research to determine the standardized value of olfactory evaluation in forensic practice.
References
Guryev SO, Kushnir VA, Satsyk SP, Grebenyuk VI. [Road traffic injury associated with alcohol intoxication (clinical and epidemiological analysis based on traffic participation)]. Trauma. 2021;22(2):21-7. Ukrainian. doi: https://doi.org/10.22141/1608-1706.2.22.2021.231954
Kirstukaitė B, Paškauskienė A, Chmieliauskas S, Laima S, Vasiljevaitė D, Stasiūnienė J. Forensic Assessment of Alcohol Intoxication in Cases of Fatal Road Traffic Accidents in Lithuania. Acta Med Litu. 2024;31(1):169-76. doi: https://doi.org/10.15388/Amed.2024.31.1.22
Chikritzhs T, Livingston M. Alcohol and the Risk of Injury. Nutrients. 2021;13(8):2777. doi: https://doi.org/10.3390/nu13082777
Ognev VA, Zinchuk AM, Chukhno IA. Analysis of the relationship between the studied characteristics of statistical aggregates. Kharkiv, Ukraine: KhNMU; 2020. 23 p. Ukrainian.
P-value calculator, chi-square [Internet]. Illinois.edu. [cited 2025 Dec 10]. Available from: http://courses.atlas.illinois.edu/fall2020/STAT200/pchisq.html
Okulevičiūtė A, Chmieliauskas S, Laubner-Sakalauskienė G, Badaras R, Laima S, Vasiljevaitė D, et al. Postmortem Analysis of Ethyl Alcohol Concentration in Blood, Urine, Muscle and Bile. Acta Med Litu. 2024;31(2):207-16. doi: https://doi.org/10.15388/Amed.2024.31.2.6
Jones AW. Evidence-based survey of the elimination rates of ethanol from blood with applications in forensic casework. Forensic Sci Int. 2010;200(1-3):1-20. doi: https://doi.org/10.1016/j.forsciint.2010.02.021
Gunathilake TB, Vidanapathirana M. Medicolegal significance of using smell of liquor as an indicator of alcohol consumption. Medico-Legal Journal of Sri Lanka. 2019;7(1):8. doi: https://doi.org/10.4038/mljsl.v7i1.7388
Kodikara S, Wijerathne S, Kudagama KM, Jayasooriya RP, Dayapala A, Subasinghe D. Legislation made four decades ago on "smelling of liquor": erroneous in the context of current scientific knowledge. Sri Lanka J Forensic Med Sci Law [Internet]. 2020;11(2):47. doi: https://doi.org/10.4038/sljfmsl.v11i2.7861
Marti V, Augsburger M, Widmer C, Lardi C. Significant postmortem diffusion of ethanol: A case report. Sci Int. 2021;328:111046. doi: https://doi.org/10.1016/j.forsciint.2021.111046
Patel D, Burr WS, Daoust B, Forbes S. Identifying the transition from ante-mortem to post-mortem odor in cadavers in an outdoor environment. Forensic Sci Int Synerg. 2025;11:100616. doi: https://doi.org/10.1016/j.fsisyn.2025.100616
Boumba VA, Ziavrou KS, Vougiouklakis T. Biochemical pathways generating post-mortem volatile compounds co-detected during forensic ethanol analyses. Forensic Sci Int. 2008;174(2-3):133-51. doi: https://doi.org/10.1016/j.forsciint.2007.03.018
Kugelberg FC, Jones AW. Interpreting results of ethanol analysis in postmortem specimens: a review of the literature. Forensic Sci Int. 2007;165(1):10-29. doi: https://doi.org/10.1016/j.forsciint.2006.05.004
Chun HJ, Poklis JL, Poklis A, Wolf CE. Development and Validation of a Method for Alcohol Analysis in Brain Tissue by Headspace Gas Chromatography with Flame Ionization Detector. J Anal Toxicol. 2016;40(8):653-8. doi: https://doi.org/10.1093/jat/bkw075
Wachholz P, Skowronek R, Pawlas N. Assessing the applicability of cerebrospinal fluid collected from the spinal cord for the determination of ethyl alcohol in post-mortem toxicology. Forensic Sci Med Pathol. 2023;19(1):44-9. doi: https://doi.org/10.1007/s12024-022-00560-8
Szeremeta M, Mironiuk E, Janica M, Drobuliakova P, Lomperta K, Szczypek M, et al. Vitreous humour as an alternative material for the determination of alcohol concentration in human corpses. Arch Med Sadowej Kryminol. 2018;68(2):108-18. English. doi: https://doi.org/10.5114/amsik.2018.77923
Andresen-Streichert H, Müller A, Glahn A, Skopp G, Sterneck M. Alcohol Biomarkers in Clinical and Forensic Contexts. Dtsch Arztebl Int. 2018;115(18):309-15. doi: https://doi.org/10.3238/arztebl.2018.0309
Jones AW. Pharmacokinetics of Ethanol - Issues of Forensic Importance. Forensic Sci Rev. 2011;23(2):91-136.
Downloads
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 O. V. Shcherbak, D. O. Kuzmina, A. V. Kis, D. O. Mokhniuk, P. O. Leontiev, L. V. Pershyna

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. 



