Population level of main microbiota and yeast-like Candida fungi in women with vulvovaginal candidiasis

Authors

  • N. D. Yakovychuk Bukovinian state medical university, Chernivtsi, Ukraine,
  • S. Ye. Dejneka Bukovinian state medical university, Chernivtsi, Ukraine,
  • I. I. Sydorchuk Bukovinian state medical university, Chernivtsi, Ukraine,
  • L. I. Sydorchuk Bukovinian state medical university, Chernivtsi, Ukraine,
  • A. V. Humenna Bukovinian state medical university, Chernivtsi, Ukraine,
  • V. V. Bendas Bukovinian state medical university, Chernivtsi, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2017.3.100867

Keywords:

microbiota, Candida, vulvovaginal candidiasis

Abstract

Aim - to determine population levels of main microbiota and yeast-like fungi of the genus Candida in healthy women and in women with vulvovaginal candidiasis.

Materials and methods. For 10 years we have been surveyed 270 women in reproductive age from 19 to 34 years old with vulvovaginal candidiasis and 73 healthy women. In all patients population level of main microflora and yeast-like fungi of the genus Candida in vulvovaginal content has been determined. The isolation of yeast-like fungi of the genus Candida has been performed by seeding of vulvovaginal content on a solid Saboaraud nutrient medium and on Saboaraud medium with the addition of cycloheximide, relatively with pure culture. "Seedlings test" and identification by species with establishment of population level had been performed in each individual case.

Results. Coexistence of representatives of the ecosystem "microorganism - microbiota" and compared the changes of microecology of vulvovaginal content in healthy women and women with vulvovaginal candidiasis were defined. In the study of population level of yeast-like fungi of the genus Candida in vulvovaginal content in women with vulvovaginal candidiasis it was found that the dominant species was C. albicans, of population level of which were different from other species, such as C. guillіermondii by 1.77 %, C. krusei - by 4.82 %, C. glabrata - by 5.78 %, C. kefyr – by 8.19 %, C. tropicalisby 8,35 %, C. inconspicua and C. zeylanoides by 15.08 %. Thus, in the vulvovaginal content of women with vulvovaginal candidiasis yeast-like fungi of the genus Candida of 8 different taxons were at the population level over 5,00 lg CFU/ml, which can not only persist but they were suspected to be agents of inflammation. The results of the study of population levels of autochtonous obligate and facultative bacteria of vulvovaginal content of women with vulvovaginal candidiasis indicated that the level of Lactobacteria decreased by 67.87 %, Bifidobacteria - by 67.27 % compared to the practically healthy women and Propionibacterium were not generally found in minimal (<4.00 lg CFU/ml) quantities. Thus, vulvovaginal candidiasis develops with impaired self-regulating functions of bacteria in the biotope.

Conclusions. Definition of indicators of microbiota of vulvovaginal content in women with vulvovaginal candidiasis made it possible to detect high population levels of yeast-like fungi of the genus Candida, such as C. albicans and violation of indigenous self-regulating functions of obligate bacteria that calls for decontamination of Candida and correction of normal flora.

 

References

Ng, K. P., Kuan, C. S., Kaur, H., Na, S. L., Atiya, N., & Velayuthan, R. D. (2015). Candida species epidemiology 2000-2013: a laboratory-based report. Trop Med Int Health., 20(11), 1447–1453, doi: 10.1111/tmi.12577.

Hani, U., Shivakumar, H.G., Vaghela, R., Osmani, R.A., & Shrivastava, A. (2015). Candidiasis: a fungal infection-current challenges and progress in prevention and treatment. Infect. Disord. Drug. Targets., 15(1), 42–52.

Macklaim, J. M., Clemente, J. C., Knight, R., Gloor, G. B., & Reid, G. (2015). Changes in vaginal microbiota following antimicrobial and probiotic therapy. Microb. Ecol. Health. Dis., 26, 277–299. doi: 10.3402/mehd.v26.27799.

Nagashima, M., Yamagishi, Y., & Mikamo, H. (2016). Antifungal susceptibilities of Candida species isolated from the patients with vaginal candidiasis. J. Infect. Chemother., 22(2), 124–126. doi: 10.1016/j.jiac.2015.08.008.

Nenadić, D., & Pavlović, M. D. (2015). Value of bacterial culture of vaginal swabs in diagnosis of vaginal infections. Vojnosanit Pregl., 72(6), 523–528. doi: 10.2298/VSP140602061N.

Hanson, L., VandeVusse, L., Jermé, M., Abad, C. L., & Safdar, N. (2016). Probiotics for Treatment and Prevention of Urogenital Infections in Women: A Systematic Review. J. Midwifery Womens Health., 61(3), 339–355, doi: 10.1111/jmwh.12472.

Dou, N., Li, W., Zhao, E., Wang, C., Xiao, Z., & Zhou, H. (2014). Risk factors for candida infection of the genital tract in the tropics. Afr. Health. Sci., 14(4), 835–839, doi: 10.4314/ahs.v14i4.10.

Bernardis, F. De., Arancia, S., Sandini, S., Graziani, S., & Norelli, S. (2015). Studies of Immune Responses in Candida vaginitis. Pathogens., 4(4), 697–707, doi: 10.3390/pathogens4040697.

Ugwa, E. A. (2015). Vulvovaginal Candidiasis in Aminu Kano Teaching Hospital, North-West Nigeria: Hospital-Based Epidemiological Study. Ann. Med. Health Sci. Res., 5(4), 274–278. doi: 10.4103/2141-9248.160185.

Schalkwyk, J., & Yudin, M. H. (2015). Vulvovaginitis: screening for and management of trichomoniasis, vulvovaginal candidiasis, and bacterial vaginosis. J Obstet Gynaecol Can., 37(3), 266–274. doi: 10.1016/S1701-2163(15)30316-9.

How to Cite

1.
Yakovychuk ND, Dejneka SY, Sydorchuk II, Sydorchuk LI, Humenna AV, Bendas VV. Population level of main microbiota and yeast-like Candida fungi in women with vulvovaginal candidiasis. Zaporozhye Medical Journal [Internet]. 2017May5 [cited 2024Nov.2];(3). Available from: http://zmj.zsmu.edu.ua/article/view/100867

Issue

Section

Original research