Application of sorption technologies for infected burn wounds treatment in the experiment
DOI:
https://doi.org/10.14739/2310-1210.2017.6.115097Keywords:
burns, sorbent, P. aeruginosaAbstract
The frequency of infectious complications in patients with burn is still an actual problem of modern medicine. Endogenous and exogenous intoxication leads to disruption organs and systems functions in general. That's why more attention should be paid to such a direction of medicine as application sorption.
Purpose: The aim of this study was the evaluation of antibacterial activity and wound-healing properties of the sorbent applicator based on highly dispersed nanosilica with immobilized antimicrobial mixture for the treatment of experimental Pseudomonas infection.
Materials and мethods: The research was carried out on 90 6-month male mice NMRI line weight 18–20 grams. All animals were divided into 5 groups. We used a model of the contact thermal burn. After burning experimental animals were infected by daily agar culture of multidrug resistant clinical strain of P. aeruginosa 3101, obtained from a patient with burns. Microbiological studies of burn wounds were carried out. The rate of wound healing was carried out using the following parameters: the average rate of wound surface decrease (cm² per day) and reduction of wound surface area (percent per day – Popova’s index).
Results. It has been found that the application of new original applicator sorbent for 3 days decrease the number of microbial cells in the wound below the critical level (105). In this experimental group the amount of purulent discharge from the wounds, swelling, redness and infiltration of tissues were reduced in comparison with the other groups. Earlier cleansing of wounds and acceleration of reparative processes were observed.
Conclusions. The findings suggest that the applicator sorbent exhibits antibacterial and wound healing properties and can be a prospective complex drug for the topical treatment of Pseudomonas infected burns.
References
Bowler, P. G., Duerden, B. I., & Armstrong, D. G. (2001) Wound microbiology and associated approaches to wound management. Clinical Microbiology Reviews, 14(2), 244–269. doi: 10.1128/CMR.14.2.244-269.2001.
Chernyshenko, T. І. (2000) Vplyv enterosheliu na vnutrіshnoklіtynnu reheneratsіiu neironіv kory holovnoho mozku pry tiazhkіi opіkovіi travmі [Influence of enterosgel on intracellular regeneration of neurons of the cerebral cortex in severe burn injury]. Medychna khіmіia, 2(3), 57–58. [in Ukrainian].
Levashov P.A., Afanasieva O.I., Dmitrieva O.A., Klesareva, E. V., Adamova, I. Yu., Afanasieva, M. I., et al.(2010) Preparation of affinity sorbents with immobilized synthetic ligands for therapeutic apheresis. Biochemistry, 4(3), 303–307. doi: 10.1134/S1990750810030133.
Baklanova, O. N., P'yanova, L. G., Talzi, V. P., Knyazheva, O. A., Sedanova, A. V., Likholobov, V. A., & Dolgikh, T. I. (2012) Modificirovanye poverkhnosti uglerodnogo sorbenta poli-N-vinilpirrolidonom dlya applikacionnoj mediciny [Modification of carbon sorbent surface by poly-N-vinylpyrrolidone in application medicine]. Fizikokhimiya poverkhnosti i zaschita materialov, 4(48), 363–369.
Belik, E. V., Brykalov, A. V., Bostanova, F. A., Gryadskikh, D. A., & Golovkina, E. M. (2008) Fabrication and study of biologically active organosilica polymer composites used for application sorption. Fibre Chemistry, 40(5), 445–446. doi: 10.1007/s10692-009-9080-7.
Chujko, A. A. (2001) Medicinskaya khimiya i klinicheskoe primenenie dioksida kremniya [Medical сhemistry and сlinical application of Silica]. Kyiv : Naukova dumka. [in Russian].
Patent 51424 Ukraina. №2002032181. Prystrii dlia modeliuvannia opikovoi khvoroby [Device for modeling of burn disease]. Biuleten, 11. [in Ukrainian].
Kochetygov, N. I. (1973) Ozhogovaya bolezn´: ocherki po patologicheskoj fiziologii [Burn disease: essays on pathophysiology]. Leningrad: Medicina. [in Russian].
Volyanskij, Yu. L., et al. (2010) Biologicheskaya kharakteristika i mikrobiologicheskaya identifikaciya nefermentiruyuschikh gramotricatel'nykh bakterij [Biological characteristics and microbiological identification of non-fermenting gram-negative bacteria]. Harkiv. [in Russian].
Chadaev, A. P., & Klimiashvili, A. D. (2002) Sovremennye metodiki mestnogo medikamentoznogo lecheniya inficirovannykh ran [Modern methods of topical treatment for infected wounds]. Russkij medicinskij zhurnal, 26, 1211. [in Russian].
Belyaeva, O. A., Neshta, V. V., Procyuk, R. R., et al. (2007) Primenenie applikacionnykh sorbentov novogo pokoleniya v gnojnoj khirurgii [Application of new generation sorbents in purulent surgery]. Klinichna khirurhiia, 11/12, 5–6. [in Russian].
Geraschenko, I. I. (2009) Membranotropnyie svoystva nanorazmernogo kremnezema [Membranotropic properties of nano-sized silica]. Poverkhnost´, 1(16), 288–306. [in Russian].
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
