Pharmacologic and toxicologic properties of lyophilic extract Achillea setacea Waldst. et Kit.

Authors

  • G. P. Smoylovska Zaporizhzhia State Medical University, Ukraine,
  • O. V. Mazulin Zaporizhzhia State Medical University, Ukraine,
  • A. V. Abramov Zaporizhzhia State Medical University, Ukraine,
  • N. V. Bukhtiyarova Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2017.6.115308

Keywords:

Achillea, hemostatic activity, phyllochinone

Abstract

Infusions based on Achillea setacea Waldst. et Kit. are traditionally used as antiinflammatory, hepatic, appetizing, spasmolytic agents, they are used as well in hemorrhages of different etiology, thrombophlebitis and varicose ulcers. Diverse pharmacologic properties are due to the presence of essential oils, proazulene, sesquiterpenic lactones, hydroxycinnamomic acids, flavonoids, vitamin K1 in medicinal raw materials. In using infusion phylloquinone, ascorbinic acid, flavonoids, hydroxycinnamomic acids, Calcium salts, tannins, achileine contribute to regulation of blood coagulation. It is very timely to assess hemostatic activity for lyophilic plant extracts which are perfectly soluble in water and have high biologic availability.


The aim of research is to assess the degree of acute toxicity and hemostatic activity for lyophilic extract of herb Achillea setacea Waldst. et Kit.


Materials and methods. Lyophilic extracts based on infusion of herb Achillea setacea Waldst. et Kit. obtained by dry sublimation have been used for the research. Lyophilic extracts obtained have been standardized for available vitamin K1 and have been studied for acute toxicity and hemostatic activity. Studying blood coagulation system has been carried out in animals with experimental toxic hepatitis for the following indices: blood coagulation time, prothrombin time, fibrin concentration in the blood.

Results. Lyophilic extract based on Achillea setacea Waldst. et Kit. is friable amorphous light yellow mass with bitter taste, containing phylloquinone in concentration 5.79 ± 0.05 %. When studying acute toxicity median lethal dose for lyophilic extracts of Achillea setacea Waldst. et Kit. was above 2000 mg/ml (practically nontoxic ones). Course therapy of extract Achillea setacea Waldst. et Kit. and solution of “Vikasol” for animals with toxic hepatitis led to decreasing hypocoagulation: decreasing prothrombin time and clotting time, increasing fibrin concentration in the blood up to 14.2 ± 1.2 mg/ml.

Conclusions. Studying lyophilic extracts of Achillea setacea Waldst. et Kit. standardized on vitamin K1 for acute toxicity allows to attribute them to VI class of toxicity. Administration of lyophilic extract Achillea setacea Waldst. et Kit. in dose 100 mg/ml resulted in decreasing hypocoagulation displaying in decreasing clotting time, in decreasing prothrombin time and increasing available fibrin in the blood. These indices exceeded by its activity reference medicine “Vikasol” (6 mg/kg).

References

Benedek, B., Gjoncaj, N., Saukel, J., & Kopp, B. (2007). Distribution of phenolic compounds in Middleeuropean taxa of the Achillea millefolium L. aggregate. Chem. Boidivers, 4(5), 849–857. doi: 10.1002/cbdv.200790072.

Taşkın, D., & Dölen, E. (2016). Antioxidant Capacity, Total Phenolic and Flavonoid Contents in flowers of Achillea setacea. International Journal of Pharmaceutical Science and Health Care, 6(3), 32–38.

Canan Karaalp, Ayse Nur Yurtman, N. Ulku Karabay Yavasoglu (2008). Evaluation of antimicrobial properties of Achillea L. flower head extracts. Pharmaceutical Biology, 47(1), 86–91.doi: 10.1080/13880200802448682.

Kyslychenko, O. А. (2014). Flavonoids determination in the aboveground part of Achillea millefolium. Ukrainskyi medychnyi almanakh, 17(3), 46–48.

Smoilovskaya, L. G. P., Mazulin, A. V., & Svetashov, O. M. (2014). Bakteriostatychna aktyvnist efirnykh olii deiakykh predstavnykiv sektsii Millefolium Koch. rodu Achillea [The bacteriostatic activity of essential oils for some specimen Millefolium Koch. genus Achillea]. Current issues in pharmacy and medicine: science and practice, 3(16), 40–45. [in Ukrainian]. doi: http://dx.doi.org/10.14739/2409-2932.2014.3.32852.

Smoylovska, H. P., & Mazulin, O. V. (2007). Spektrofotometrychne vyznachennia vitaminu K u travi vydiv rodu Achillea L. [Spectrophotometric determination of vitamin K in the herb of Achillea L. species]. Farmatsevtychnyi chasopys, 1(1), 101–103. [in Ukrainian]. doi: http://dx.doi.org/10.11603/2312-0967.2007.1.3127.

Stefanov, A. V. (2002). Doklinicheskie issledovaniya lekarstvennykh sredstv [Preclinical studies of medicinal products]. Kyiv: Avicenna [in Russian].

Kozlov, A. A., Natrus, L. V., Chernovol, P. A., Melkumian, A. L., Konoplianko, V. A., Snehir, M. A., & Prostakova, T. M. (2011). Laboratornaya diagnostika sistemy gomeostaza [Laboratory diagnostics of homeostasis]. Мoscow: Littera [in Russian].

Downloads

How to Cite

1.
Smoylovska GP, Mazulin OV, Abramov AV, Bukhtiyarova NV. Pharmacologic and toxicologic properties of lyophilic extract Achillea setacea Waldst. et Kit. Zaporozhye Medical Journal [Internet]. 2017Nov.15 [cited 2025Feb.3];(6). Available from: http://zmj.zsmu.edu.ua/article/view/115308

Issue

No. 6 (2017)

Section

Problems of pharmacy

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)