Microscopic and ultramicroscopic changes of the gastric mucosa chief exocrinocytes under the influence of sublethal general dehydration of the organism

Authors

  • V. I. Hula Sumy State University, Sumy, Ukraine,
  • V. Z. Sikora Sumy State University, Sumy, Ukraine,
  • O. S. Yarmolenko Sumy State University, Sumy, Ukraine,
  • V. I. Bumeister Sumy State University, Sumy, Ukraine,
  • M. S. Pernakov Sumy State University, Sumy, Ukraine,
  • V. O. Boiko Sumy State University, Sumy, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2018.02.124948

Keywords:

stomach, dehydration, exocrine cells

Abstract

The functional state of the gastric mucosa chief cells reflects the ability to break down proteins and fully absorb the substances which are vital for the body under the influence of severe general dehydration of the organism.

The aim To reveal morphological and ultramicroscopic changes of the gastric fundus chief exocrinocytes of rats under the influence of sublethal general dehydration of organism.

Materials and methods. The influence of sublethal general dehydration was modeled on mature laboratory rats of the experimental series that was achieved on the 9th day of the experiment. During the experiment the animals were kept based on a complete waterless regime and fed dry granulated food. Samples for microscopic and ultramicroscopic studies were prepared according to generally accepted techniques. The results were evaluated using light microscope "Olympus" with a photographic registration of the morphological picture by a Baumer / optronic video camera. Type: CX05c and by electron microscope EM-125. Morphometric studies were carried out using the program «Digimizer».

Results. The average values of the cross-sectional area of the chief cells cytoplasm and nuclei, their microscopic and ultramicroscopic changes under influence of severe general dehydration of the organism were studied.

Conclusions. Severe general dehydration of the organism is accompanied by a decrease in the total number of the oxyntic cell in gastric glands by 21.22 % (P < 0.0001) and in the number of chief exocrinocytes per one gland by 19.43 % (P < 0.0001), relative to the control group. Percentage of the chief exocrinocytes in the gastric glands on the 9th day of the experiment is 37.66 %. A morphometric study has revealed a decrease in the cross-sectional area of the cells cytoplasm by 37.73 % (P < 0.0001), of nuclei by 21.97 % (P = 0.0110).

Ultrastructural transformations of the chief exocrinocytes are signs of a sharp decrease in synthetic processes intensity in these cells, as well as a violation of not only the secret synthesis, but also the process of its secretion from the cytoplasm. The revealed ultrastructural changes in the cell organelles of the gastric fundic glands chief exocrinocytes are results of hypoxic alteration and metabolic disturbances (as general dehydration manifestations) and are destructive-dystrophic with the transformation into necrobiotic nature.

References

Dudnyk, S. V., & Koshelia, I. I. (2016). Tendentsii stanu zdorovia naselennia Ukrainy [Health’ state tendencies of population in Ukraine]. Ukraina. Zdorovia natsii, 4, 67–77. [in Ukrainian].

Shafranskyi, V. V. (Ed.) (2016). Shchorichna dopovid pro stan zdorovia naselennia, sanitarno-epidemichnu sytuatsiiu ta rezultaty diialnosti systemy okhorony zdorovia Ukrainy. 2015 rik [Annual report of the health status of the population, the sanitary and epidemiological situation and the results of the health care system of Ukraine. 2015 year]. Kyiv. [in Ukrainian].

Prykhodko, O. O., Hula, V. I., Yarmolenko, O. S., Pernakov, M. S., Sulim, L. G., Bumeister, V. I., et al. (2016). Mikroskopicheskie izmeneniya organov krys v usloviyakh obshhej degidratacii organizma [Microscopic changes in rat organs under conditions of total dehydration]. Azerbaijan Medical Journal (AТJ), 4, 95–100. [in Russian].

Hula, V. I. (2016). Strukturna perebudova fundalnoho viddilu shlunku shchuriv za umov klitynnoho znevodnennia orhanizmu [Structural alteration of rats' stomach fundus under condition of intracellular dehydration of the body]. Zhurnal klinichnykh ta eksperymentalnykh medychnykh doslidzhen, 4(4), 537–545. [in Ukrainian].

Hula, V. I., & Sikora, V. Z. (2016). Histomorfometrychnyi analiz zmin stinky shlunku za umov zahalnoi dehidratatsii orhanizmu [Histomorphometric analysis of stomach walls alternation under conditions of general body dehydration]. Morphologia, 10(4), 23–28. [in Ukrainian].

Merkulov, G. A. (1969). Kurs patogistologicheskoj tekhniki [Course of pathogistological technique]. Leningrad: Medicina. [in Russian].

Kuo, J. (Ed.) (2007) Electron microscopy: methods and protocols. Methods in molecular biology. Totowa: Humana Press Inc. Publ.

Davydova, L. M., Muravskyi, D. V., & Maksymova, O. S. (2016) Ultramorfolohichna perebudova miazovykh volokon yazyka shchuriv pry dehidratsiinykh porushenniakh orhanizmu [Ultramorphological reconstruction of muscle fibers of the tongue rats with dehydration disorders of the body]. Aktualni pytannia teoretychnoi ta praktychnoi medytsyny. (Vol. 1). (P. 27–28). Sumy [in Ukrainian].

Bochkarnikova, N. V., & Alfonsova, E. V. (2013) Strukturnaya organizaciya zheludka pri metabolicheskom acidoze [The structural organization of gaster in metabolic acidosis]. Sovremennye problemy nauki i obrazovanyia, 6, 680. [in Russian].

Soboleva, A. D. (1975) Reakciya kletok i tkanej na obezvozhivanie [Reaction of cells and tissues to dehydration]. Moscow: Nauka. [in Russian].

Myslyaeva, T. G. (Ed.) (1981) Patofizioloiya obezvozhivaniya organizma [Pathophysiology of dehydration of an organism]. Moscow [in Russian].

Berkhin, E.B., & Ivanov, Yu. I. (1972) Metody e'ksperimental'nogo issledovaniya pochek i vodno-solevogo obmena [Methods of experimental study of kidneys and water-salt metabolism]. Barnaul. [in Russian].

Pogorelov, M. V., Bumeyster, V. I., Tkach, G. F., Bolotna, I. V., & Bonchev, S. D. (2009) Suchasni uiavlennia pro vodno-solovyi obmin (ohliad literatury ta metody vlasnykh doslidzhen) [Modern imagines about aqua-mineral balans (literature review and methods of proper reserches)]. Visnyk problem biolohii ta medytsyny, 2, 8–14. [in Ukrainian].

Bakes, K., Haukoos, J. S., Deakyne, J. S., Hopkins, E., Easter, J., McFann, K., et al. (2016) Effect of Volume of Fluid Resuscitation on Metabolic Normalization in Children Presenting in Diabetic Ketoacidosis: A Randomized Controlled Trial. The Journal of Emergency Medicine, 50(4), 551–9. doi: 10.1016/j.jemermed.2015.12.003.

Singhi, S. C., Shah R., Bansal A., & Jayashree M. (2013) Management of a child with vomiting. Indian J Pediatr, 80(4), 318–25. doi: 10.1007/s12098-012-0959-6.

Dvorshchenko, K. O., Savko, U. V., Vakal, S. Ye., Todor, I. M., & Ostapchenko,L. I. (2008) Strukturno-funktsionalnyi stan mitokhondrii pechinky shchuriv za umov vyrazkovykh urazhen shlunka [Structural-functional state of rats liver`s mitochondrias under conditions of gastric ulcer]. Fizyka zhyvoho, 16(2), 112–115. [in Ukrainian].

De Luca, Jr. L. A., Vendramini, R. C., Pereira D. T., Colombari, D. A., David, R. B., de Paula, P. M., et al. (2007) Water deprivation and the double depletion hypothesis: common neural mechanisms underlie thirst and salt appetite. Brazilian Journal of Medical and Biological Research, 40, 707–712.

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1.
Hula VI, Sikora VZ, Yarmolenko OS, Bumeister VI, Pernakov MS, Boiko VO. Microscopic and ultramicroscopic changes of the gastric mucosa chief exocrinocytes under the influence of sublethal general dehydration of the organism. Zaporozhye Medical Journal [Internet]. 2018Mar.7 [cited 2024Nov.23];(2). Available from: http://zmj.zsmu.edu.ua/article/view/124948

Issue

No. 2 (2018)

Section

Original research

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