The effect of estrogen deficiency and its combination with chronic stress on the condition of periodontal in old rats

Authors

  • N. M. Savielieva Kharkiv National Medical University, Ukraine,
  • O. V. Honcharenko Odesa National Medical University, Ukraine,
  • S. A. Schnaider SI “Institute of Dentistry and Maxillofacial Surgery of the National Academy of Medical Sciences of Ukraine”, Odesa,
  • Ye. K. Tkachenko SI “Institute of Dentistry and Maxillofacial Surgery of the National Academy of Medical Sciences of Ukraine”, Odesa,

DOI:

https://doi.org/10.14739/2310-1210.2019.1.155832

Keywords:

estrogens, ovariectomy, stress, lipid peroxidation, histomorphometry, periodontal diseases, rats

Abstract

The aim of the study was to investigate the effect of ovariectomy and its combination with stress (femoral fracture) on the periodontal condition in old rats.

Materials and methods. A total of 24 white female rats were used in the experiment. The first group consisted of intact rats. Rats of the second group were subjected to an ovariectomy (OE) at the age of 2 months. Rats of the third group underwent a fracture of the one femur 1 month before they were euthanized. The animals were sacrificed at 15 months of age.

Results. The level of LPO was increased in the liver, salivary glands and alveolar bone process after experimental ovariectomy; the recession defects on molars were significantly increased. Stress (femoral fracture) aggravated the intensification of peroxidation processes in the salivary glands and liver of rats. The levels of nucleic and higher polyene fatty acids in the liver lipids were decreased in the combination of ovariectomy and stress. The level of LPO in the alveolar bone process was not changed significantly. Histomorphometric study of periodontium in rats revealed a decrease in the upper margin of epithelial attachment.

Conclusions. Experimental estrogen deficiency caused the most significant pathogenic effect on the periodontium in old rats. The obtained data demonstrate that female sex hormone deficiency is one of the mechanisms in periodontitis pathogenesis.

 

References

Baclum, V., & Lopez, R. (2013) Periodontal disease epidemiology-learned and unlearned? Periodontal, 62(1), 37–58. doi: 10.1111/j.1600-0757.2012.00449.x.

Smetnik, V. P., & Smetnik, A. A. (2013) E'strogeny i kostnaya tkan' [Estrogens and bone tissue]. Farmateki, 12, 17–21. [in Russian].

Suri, V. (2014) Menopause and oral health. J. Midlife Health, 5(3), 115–120. doi: 10.4103/0976-7800.141187.

Sherbakov, I. V., Oreshaka, O. V., & Dementyeva, E. A. (2015) Izmeneniya sostoyaniya tkanej parodonta u zhenshchin pri vyrazhennom deficite e'strogenov [The state alteration of women's periodontal tissues in the presence of the frank estrogen deficiency]. Institut stomatologii, 1(66), 70–71. [in Russian].

Кorenevskaya, N. A. (2009) Vozdejstvie stressa na sostoyanie tkanej polosti rta. Aktual'nye problemy ehksperimental'noj i klinicheskoj mediciny [The impact of stress on the condition of the tissues of the mouth. Actual problems of experimental and clinical medicine]. Aktual'nye problemy e'ksperimental'noj i klinicheskoj mediciny Proceedings of the 67th Scientific and Practical Conference. Volgograd: Izd-vo VolGMU. [in Russian].

Kindlova M., Mrklas L., Maricova Z., Broukal Z. (1981) Age-dependent changes in the rat periodontium, J. of Periodont. res.16, 497-503.

Vladimirov, Yu. A., & Archakov, A. I. (1972) Perekisnoe okislenie lipidov v biologicheskikh membranakh [Peroxide oxidation of lipids in biological membranes]. Мoscow : Nauka. [in Russian].

Stal'naya, I. D., & Garishvili, T. (1977) Metod opredeleniya dienovykh konyugacij nenasyschennyh vysshikh zhirnykh kislot [The method for determining diene conjugations of unsaturated higher fatty acids]. Sovremennye metody biokhimi, (Orekhovich, V. N., ed), (P. 63–64). Moscow. [in Russian].

Putilina, E. F. (1982) Opredelenie aktivnosti glutation-reduktazy [Determination of glutathione reductase activity]. Metody biokhimicheskikh issledovanij. Moscow. [in Russian].

Pahomova, V., Kozlyanina, N., & Kryukova, G. (1982) Аvtorskoe svidetel'stvo 922637 SSSR. МКИ 01 33/48. Sposob opredeleniya aktivnosti glutation-peroksidazy v biologicheskikh tkanyakh [The method for determining the activity of glutathione peroxidase in biological tissues]. Biulleten, 15. [in Russian].

Trudolyubova, M. G. (1977) Kolichestvennoe opredelenie RNK i DNK v subkletochnykh frakciyakh kletok zhivotnykh [Quantification of RNK and DNK in subcellular fractions of animal cells]. Sovremennye metody v biokhimii. Moscow: Medicina. [in Russian].

Matusis, L. I. (1988) Issledovanie zhirno kislotnogo sostava lipidov kozhi metodom gazo-zhidkostnoj khromatografii [Study of fatty acid composition of skin lipids by gas-liquid chromatography]. Laboratornoe delo, 6, 26–30. [in Russian].

Maksimovskij, Yu. M., Motil' A. I., & Novikov, V. E. (1991) Sostoyanie parodonta pri generalizovannom osteoporoze u zhenshchin s nedostatochnoj funkciej yaichnikov [Condition of periodontal disease in generalized osteoporosis in women with insufficient ovarian function]. Stomatologiya, 1, 24–26. [in Russian].

Tarasenko, L. M. (1985) Patogenez povrezhdeniya parodonta pri stresse. (Avtoref. dis… dokt. med. nauk) [Pathogenesis of periodontal damage during stress. Dr. med. sci. diss.]. Moscow. [in Russian].

Koronevskaya, N. A., & Gorodeckaya, I. V. (2009) Vliyanie stressa na sostoyanie tkanej chelyustno-licevoj oblasti [The influence of stress on the state of the tissues of the maxillofacial region]. Vestnik Vitebskogo gosudarstvennogo medicinskogo universiteta, 8(3), 1–20. [in Russian].

Antonova, J. N. (2008) Changes in the masticatory muscles periodontal tissues, and the pharyngeal ring in Wistar rats in chronic psychophysical stress. Neurosci. Behav. Phisiol, 38(2), 891–896. doi: 10.1007/s11055-008-9068-4.

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How to Cite

1.
Savielieva NM, Honcharenko OV, Schnaider SA, Tkachenko YK. The effect of estrogen deficiency and its combination with chronic stress on the condition of periodontal in old rats. Zaporozhye Medical Journal [Internet]. 2019Feb.8 [cited 2024Nov.22];(1). Available from: http://zmj.zsmu.edu.ua/article/view/155832

Issue

No. 1 (2019)

Section

Original research

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