Structural changes in the wall of the abdominal aorta in modeling of long-term cannabinoid receptors CB1 blockade in laboratory animals
DOI:
https://doi.org/10.14739/2310-1210.2019.3.169186Keywords:
abdominal aorta, cannabinoid receptorsAbstract
The purpose of the work is to detect the effect of long-term cannabinoid receptors CB1 blockade on the abdominal aorta wall structure in experimental rats.
Materials and methods. The study was performed on two groups of 100-day-old male Wistar rats. The first group was made up of intact animals. The second group consisted of animals that were administrated with rimonabant hydrochloride solution (10 mg/kg-1 of body weight) per os with drinking water per day. The experiment duration was 10 days, and then the abdominal aorta of each animal was isolated for the histological measurements. Computer morphometry, the study of the subendothelial layer thickness with the internal elastic lamina and media, the ratio of the lumen diameter to the vessel wall were conducted in the abdominal aorta wall structure assessment.
Results. It has been established that long-term cannabinoid CB1 receptors blockade in 100-day-old male Wistar rats resulted in morphological changes in all layers of the abdominal aorta wall that manifested in alterations of endothelial cells structure and their desquamation in the intima. The inner elastic lamina became thin and discontinuous. The thickness of the media was not uniform and there was a decrease in the number of elastic laminae as well as an increase in the number of chaotically oriented smooth muscle cells. Changes in adventitia manifested in swelling and loosely packed fibers with their increased dispersion. The number of collapsed vasa vasorum was decreased to single and hypertrophy of the nerve ganglia was observed. There was a thinning of the internal lamina and a decreased percentage of the lumen and vessel components of the wall at the expense of other tissues.
Conclusions. The study results indicate that the long-term cannabinoid CB1 receptors blockade is a factor that damages endothelium of the abdominal aorta and leads to a decrease in elastin and proliferation of smooth muscle cells in the media, as well as a decrease in the percentage of the lumen and wall of the abdominal aorta at the expense of other tissues.
References
Costantino, S., Paneni, F., Cosentino, F. (2016) Ageing, metabolism and cardiovascular disease. J Physiol. 594(8), 2061–2073. doi: 10.1113/JP270538
Oleĭnikov, V. É., Matrosova, I. B., Gusakovskaia, L. I., & Sergatskaia, N. V. (2014) Rol' opredeleniya aortal'nogo davleniya i rigidnosti aorty u pacientov s serdechno-sosudistymi zabolevaniyami [Role of the determination of aortic pressure and rigidity aortic in patients with cardiovascular diseases]. Terapevticheskij arkhiv, 86(4), 91–95. [in Russian].
Mikael, L. R., Paiva, A. M. G., Gomes, M. M., Sousa, A. L. L., Jardim, P. C. B. V., Vitorino, P. V. O., et al. (2017) Vascular Aging and Arterial Stiffness. Arq Bras Cardiol., 109(3), 253–258. doi: 10.5935/abc.20170091
Scioli, M. G., Bielli, A., Arcuri, G., Ferlosio, A., & Orlandi, A. (2014) Ageing and microvasculature. Vasc Cell., 6, 19. doi: 10.1186/2045-824X-6-19
Toczek, M., Baranowska-Kuczko, M., Grzęda, E., Pędzińska-Betiuk, A., Weresa, J., & Malinowska, B. (2016) Age-specific influences of chronic administration of the fatty acid amide hydrolase inhibitor URB597 on cardiovascular parameters and organ hypertrophy in DOCA-salt hypertensive rats. Pharmacol Rep., 68(2), 363–369. doi: 10.1016/j.pharep.2015.10.004
Karpińska, O., Baranowska-Kuczko, M., Kloza, M., & Kozłowska, H. (2018) Endocannabinoids modulate Gq/11 protein-coupled receptor agonist-induced vasoconstriction via a negative feedback mechanism. J Pharm Pharmacol., 70(2), 214–222. doi: 10.1111/jphp.12854
Lipina, C., & Hundal, H. S. (2017) The endocannabinoid system: 'NO' longer anonymous in the control of nitrergic signalling? J Mol Cell Biol., 9(2), 91–103. doi: 10.1093/jmcb/mjx008
Szekeres, M., Nadasy, G. L., Turu, G., Soltesz-Katona, E., Toth, Z. E., Balla, A., et al. (2012) Angiotensin II induces vascular endocannabinoid release, which attenuates its vasoconstrictor effect via CB1 cannabinoid receptors. J Biol Chem., 287(37), 31540–31550. doi: 10.1074 / jbc.M112.346296
Schaich, C. L., Shaltout, H. A., Brosnihan, K. B., Howlett, A. C., & Diz, D. I. (2014) Acute and chronic systemic CB1 cannabinoid receptor blockade improves blood pressureregulation and metabolic profile in hypertensive (mRen2)27 rats. Physiol Rep., 2(8), e12108. doi: 10.14814/phy2.12108
O'Leary, D. H., Reuwer, A. Q., Nissen, S. E., Després, J. P., Deanfield, J. E., Brown, M. W., et al. (2011) Effect of rimonabant on carotid intima-media thickness (CIMT) progression in patients with abdominal obesity and metabolic syndrome: the AUDITOR Trial. Heart., 97(14), 1143–1150. doi: 10.1136 / hrt.2011.223446
Gavreliuk, S. V. (2017) Vliyanie blokady SV1 receptorov kannabinoidov na parametry gemodinamiki i funkciyu e'ndoteliya pri immobilizacionnom stresse v e'ksperimente [Effect of cannabinoids CB1 receptors blockade on hemodynamic parameters and endothelial function at the immobilization stress in the experiment]. Zaporozhye medical journal, 6, 743–47. doi: 10.14739/2310-1210.2017.6.114704 [in Russian].
Gavreliuk, S. V. (2017) Izmenenie ul'trazvukovykh kharakteristik gemodinamicheskikh parametrov i funkcii e'ndoteliya bryushnoj aorty v e'ksperimente s dlitel'noj blokadoj SV1 receptorov kannabinoidov [Changes the ultrasonic characteristics of hemodynamic parameters and abdominal aorta endothelial function in experiment with a long-term blockade of CB1 cannabinoid receptors]. Visnyk problem biolohii i medytsyny, 1(135), 113–17. [in Russian].
Gavreliuk, S. V., & Chykina, I. V. (2017) Vplyv khronichnoho immobilizatsiinoho stresu na rozvytok dysfunktsii endoteliiu v eksperymenti [Effect of chronic immobilization stress on the development of endothelial dysfunction in rats]. Fiziolohichnyi zhurnal, 63(2), 56–64. [in Ukrainian].
Malkov, P. G., & Frank, G. A. (Eds.) (2011). Osnovy obespecheniya kachestva v gistologicheskoj laboratornoj tekhnike: Rukovodstvo [Fundamentals of quality assurance in histological laboratory equipment: Management]. Mosсow. [in Russian].
Avtandilov, G. G. (1990). Medicinskaya Morfometriya. Rukovodstvo [Medical Morphometry. Management] (384). Mosсow: Medicina. [in Russian].
Mozaffarian, D., Benjamin, E. J., Go, A. S., Arnett, D. K., Blaha, M. J., Cushman, M., et al. (2015) Heart disease and stroke statistics–2015 update: a report from the American heart association. Circulation, 131, 434–441. doi: 10.1161/CIR.0000000000000157
van Varik, B. J., Rennenberg, R. J., Reutelingsperger, C. P., Kroon, A. A., de Leeuw, P. W., & Schurgers, L. J. (2012) Mechanisms of arterial remodeling: lessons from genetic diseases. Front Genet., 3, 290. doi: 10.3389/ fgene.2012.00290
Stenmark, K. R., Yeager, M. E., El Kasmi, K. C., Nozik-Grayck, E., Gerasimovskaya, E. V., Li, M., et al. (2013) The adventitia: essential regulator of vascular wall structure and function. Annu Rev Physiol., 75, 23–47. doi: 10.1146/annurev-physiol-030212-183802
Hu, Y., & Xu, Q. (2011) Adventitial Biology. Differentiation and Function. Arteriosclerosis, Thrombosis, and Vascular Biology., 31, 1523–1529. doi: 10.1161/ATVBAHA.110.221176
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)