Embryo culture in step single and sequential media: influence on morphology and implantation potential of blastocysts
DOI:
https://doi.org/10.14739/2310-1210.2019.5.179426Keywords:
embryos, frequency implantation, embryotransferAbstract
Purpose. To assess the impact of step single and sequential culture media on the morphology and frequency of blastocyst implantation.
Materials and methods. In total, 514 embryos obtained from patients aged 30 to 35 years and fertilized by ICSI were studied (2017 to 2018). The pool of embryos from each woman was randomly divided into two groups: the first group was cultured in the sequential media and the second - in the single ones. Evaluation of the effect was carried out by comparing the obtained blastocysts quality cultured in the single and sequential media. Blastocyst morphology was assessed using the modified Gardner D. K. scale. Statistical analysis was performed by Mann–Whitney U-test and Pearson's chi-squared test with Yates' correction. Statistically significant differences between the compared groups were considered at P < 0.05.
Results. The studies found that the use of single Origio® 1-Step medium at embryo culture phase improves the morphological quality of the resulting blastocyst compared to embryos cultured in sequential Origio® Sequential Cleav™ / Sequential Blast™ media. No statistically significant difference in the pregnancy and implantation rate of blastocysts obtained after embryo cultivation in the step single or sequential media was detected.
Conclusions. The priority in terms of obtaining a large number of high quality blastocysts with high implantation potential is to use a step single medium for the embryo cultivation.
References
Stamenov, G. S., Parvanov, D. A., Chaushev, T. A., Baltadzhieva, D., Iliev, I. G., & Dzhambazov, B. M. (2013). Approaches for prediction of the implantation potential of human embryos. Journal of Bioscience and Biotechnology, 2(2), 79–88. doi: 10.3897/http://www.jbb.uni-plovdiv.bg/2013-2-2-79-88?inheritRedirect=true
Hatırnaz, S., & Pektas, M. K. (2017). Day 3 embryo transfer versus day 5 blastocyst transfers: A prospective randomized controlled trial. Turkish Journal Of Obstetrics And Gynecology, 14(2), 82–88. doi: 10.4274/tjod.99076
Lee, J. W., Cha, J. H., Shin, S. H., Kim, Y. J., Lee, S. K., Park, Ch. K., et al. (2017). Efficacy of embryo transfer on day 2 versus day 3 according to maternal age in patients with normal ovarian response. Clinical and Experimental Reproductive Medicine, 44(3), 141–145. doi: 10.5653/cerm.2017.44.3.141
Li, R.-S., Hwu, Y.-M., Lee, R. K.-K, Li, S.-H., & Lin, M.-H. (2018). Day 4 good morula embryo transfer provided compatible live birth rate with day 5 blastocyst embryo in fresh IVF/ET cycles. Taiwanese Journal of Obstetrics & Gynecology, 57(1), 52–57. doi: 10.1016/j.tjog.2017.12.008
Schwarzler, P., Zech, H., Auer, M., Pfau, K., Göbel, G., Vanderzwalmen, P., & Zech, N. (2004). Pregnancy outcome after blastocyst transfer as compared to early cleavage stage embryo transfer. Human Reproduction, 19(9), 2097–2102. doi: 10.1093/humrep/deh398
Kang, S. M., Lee, S. W., Jeong, H. J., Yoon, S. H., Koh, M. W., Lim, J. H., & Lee, S. G. (2012). Clinical outcomes of elective single morula embryo transfer versus elective single blastocyst embryo transfer in IVF-ET. Journal of Assisted Reproduction and Genetics, 29(5), 423–428. doi: 10.1007/s10815-012-9736-8
Yao, T., & Asayama, Y. (2016). Human preimplantation embryo culture media: past, present, and future. Journal of Mammalian Ova Research, 33(1), 17–34. doi: 10.1274/jmor.33.17
Sfontouris, I. A., Kolibianakis, E. M., Lainas, G. T., Petsas, G. K., Tarlatzis, B. C., & Lainas, T. G. (2017) Blastocyst development in a single medium compared to sequential media: a prospective study with sibling oocytes. Reproductive Sciences, 24(9), 1312–1318. doi: 10.1177/1933719116687653
Takashima, A., Kimura, F., Kishida, K., Izuno, M., Hirata, K., Takebayashi, A., et al. (2017). Comparison of embryo development and pregnancy rates in continuous single and sequential media cultures of sibling embryos. Journal of Advanced Medical Sciences and Applied Technologies, 3(3), 147–154.
Bouillon, C., Leandri, R., Desch L., Ernst, A., Bruno, C., Cerf, C., et al. (2016). Does Embryo Culture Medium Influence the Health and Development of Children Born after In Vitro Fertilization? PLOS ONE, 11(3), 1–14. doi: 10.1371/journal.pone.0150857
Werner, M. D., Hong, K. H., Franasiak, J. M., Forman, E. J., Reda, C. V., Molinaro, T. A., et al. (2016). Sequential versus Monophasic Media Impact Trial (SuMMIT): a paired randomized controlled trial comparing a sequential media system to a monophasic medium. Fertility and Sterility, 105(5), 1215–1221. doi: 10.1016/j.fertnstert.2016.01.005
Gardner, D. K., Schoolcraft, W. B., Wagley, L., Schlenker, T., Stevens, J., & Hesla, J. (1998). A prospective randomized trial of sequential blastocyst culture and transfer in in-vitro sequential fertilization. Human Reproduction, 13(12), 3434–3440. doi: 10.1093/humrep/13.12.3434
Ivec, M., Kovacic, B., & Vlaisavljevic, V. (2011). Prediction of human blastocyst development from morulas with delayed and/or incomplete compaction. Fertility and Sterility, 96(6), 1473–1478. doi: 10.1016/j.fertnstert.2011.09.015
Qi, S.T., Liang, L. F., Xian, Y. X., Liu, J. Q., & Wang, W. (2014). Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age. Journal of Ovarian Research, 7, 65. doi: 10.1186/1757-2215-7-65
Desai, N., Goldberg, J. M., Austin, C., & Falcone, T. (2018) Are cleavage anomalies, multinucleation, or specific cell cycle kinetics observed with time-lapse imaging predictive of embryo developmental capacity or ploidy? Fertility and Sterility, 109(4), 665–674. doi: 10.1016/j.fertnstert.2017.12.025
Lee, M.-J., Lee, R. K.-K., Lin, M.-H., & Hwu, Y.-M. (2012). Cleavage speed and implantation potential of early-cleavage embryos in IVF or ICSI cycles. Journal of Assisted Reproduction and Genetics, 29(8), 745–750. doi: 10.1007/s10815-012-9777-z
Hashimoto, S., Nishihara, T., Murata, Y., Oku, H., Nakaoka, Y., Fukuda, A., & Morimoto, Y. (2008). Medium without Ammonium Accumulation Supports the Developmental Competence of Human Embryos. Journal of Reproduction and Development, 54(5), 370–374. doi: 10.1262/jrd.20012
Li, Q., Wang, W. J., Zhang, N. F., Ouyang, N. Y., Li, R. Q., Mai, M. Q., et al. (2013). Ammonium Concentration of Spent Medium Provides a Noninvasive Assessment of Embryonic Developmental Potential in IVF. Reproductive Sciences, 20(11), 1316–1320. doi: 10.1177/1933719113483016
Lambers, M. J., Mager, E., Goutbeek, J., McDonnell, J., Homburg, R., Schats, R., et al. (2006). Factors determining early pregnancy loss in singleton and multiple implantations. Human Reproduction, 22(1), 275–279. doi: 10.1093/humrep/del367
Aghajanova, L., Hamilton, A. E., & Giudice, L. C. (2008). Uterine receptivity to human embryonic implantation: Histology, biomarkers, and transcriptomics. Seminars in Cell & Developmental Biology, 19(2), 204–211. doi: 10.1016/j.semcdb.2007.10.008
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