Association analysis between rs1899663 HOTAIR gene polymorphism and bladder cancer development in Ukrainian population
DOI:
https://doi.org/10.14739/2310-1210.2019.6.186498Keywords:
long noncoding RNAs, single nucleotide polymorphism, HOX antisense intergenic RNA (HOTAIR), cell bladder cancerAbstract
HOX antisense intergenic RNA (HOTAIR), the well-known long noncoding RNA (lnRNA), performs epigenetic regulation of cell cycle genes expression. Based on this fact it can be assumed that HOTAIR single nucleotide polymorphisms (SNPs) affect the development of different oncological processes, in particular, bladder cancer.
The aim of the study: to analyze the association between HOTAIR rs1899663 single nucleotide polymorphism and bladder cancer emergence as well as metastasis potential in Ukrainian population.
Materials and methods. Venous whole blood from 241 patients was used for genotyping. The studied group was formed from 141 patients with diagnosed bladder cancer and the control group was consisted of 100 healthy subjects. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) was used for genotyping. The statistical processing of obtained results was done by SPSS 17.0 software.
Results. There was no association between HOTAIR rs1899663 SNP and bladder cancer emergence according to the binary logistic regression analysis as well as after adjustment for age, sex and smoking (Pc > 0.05 and Pa > 0.05 for all hereditary models). In contrast, it was shown that the TT-genotype carriers have decreased risk of bladder cancer metastasis compared to G-allele carriers in the recessive (Рс = 0.047; ORс = 0.334, 95 % СІ = 0.113–0.986) model. Moreover, TT-genotype carriers have decreased risk of bladder cancer metastasis in comparison with GG-genotype carriers according to the additive (Рс = 0.04; ORс = 0.301, СІ = 0.096–0.944) model. But after the adjustment for age, sex, smoking and alcohol consumption, the statistically significant association was lost both in recessive (Рa = 0.09; ORa = 0.386; 95 % СІ = 0.129–1.159) and additive (Рa = 0.076; ORa = 0.348; СІ = 0.108–1.118) models.
Conclusions. There is no association between HOTAIR rs1899663 SNP and bladder cancer emergence as well as metastasis potential in Ukrainian population.
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