Association analysis between rs1899663 HOTAIR gene polymorphism and bladder cancer development in Ukrainian population

Authors

  • A. D. Volkohon Sumy State University, Ukraine,
  • Ya. D. Chumachenko Sumy State University, Ukraine,
  • V. Yu. Harbuzova Sumy State University, Ukraine,
  • O. V. Ataman Sumy State University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2019.6.186498

Keywords:

long noncoding RNAs, single nucleotide polymorphism, HOX antisense intergenic RNA (HOTAIR), cell bladder cancer

Abstract

 

HOX antisense intergenic RNA (HOTAIR), the well-known long noncoding RNA (lnRNA), performs epigenetic regulation of cell cycle genes expression. Based on this fact it can be assumed that HOTAIR single nucleotide polymorphisms (SNPs) affect the development of different oncological processes, in particular, bladder cancer.

The aim of the study: to analyze the association between HOTAIR rs1899663 single nucleotide polymorphism and bladder cancer emergence as well as metastasis potential in Ukrainian population.

Materials and methods. Venous whole blood from 241 patients was used for genotyping. The studied group was formed from 141 patients with diagnosed bladder cancer and the control group was consisted of 100 healthy subjects. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) was used for genotyping. The statistical processing of obtained results was done by SPSS 17.0 software.

Results. There was no association between HOTAIR rs1899663 SNP and bladder cancer emergence according to the binary logistic regression analysis as well as after adjustment for age, sex and smoking (Pc > 0.05 and Pa > 0.05 for all hereditary models). In contrast, it was shown that the TT-genotype carriers have decreased risk of bladder cancer metastasis compared to G-allele carriers in the recessive (Рс = 0.047; ORс = 0.334, 95 % СІ = 0.113–0.986) model. Moreover, TT-genotype carriers have decreased risk of bladder cancer metastasis in comparison with GG-genotype carriers according to the additive (Рс = 0.04; ORс = 0.301, СІ = 0.096–0.944) model. But after the adjustment for age, sex, smoking and alcohol consumption, the statistically significant association was lost both in recessive (Рa = 0.09; ORa = 0.386; 95 % СІ = 0.129–1.159) and additive (Рa = 0.076; ORa = 0.348; СІ = 0.108–1.118) models.

Conclusions. There is no association between HOTAIR rs1899663 SNP and bladder cancer emergence as well as metastasis potential in Ukrainian population.

 

References

Vos, T., Allen, C., Arora, M., Barber, R., Bhutta, Z., & Brown, A. et al. (2016). Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015. The Lancet, 388(10053), 1545-1602. doi: 10.1016/s0140-6736(16)31678-6

Fitzmaurice, C., Allen, C., Barber, R., Barregard, L., Bhutta, Z., & Brenner, H. et al. (2017). Global, Regional, and National Cancer Incidence, Mortality, Years of Life Lost, Years Lived With Disability, and Disability-Adjusted Life-years for 32 Cancer Groups, 1990 to 2015: A Systematic Analysis for the Global Burden of Disease Study. JAMA Oncology, 3(4), 524-548. doi: 10.1001/jamaoncol.2016.5688

Motofei, I. (2018). Biology of Cancer; From Cellular Cancerogenesis to Supracellular Evolution of Malignant Phenotype. Cancer Investigation, 36(5), 309-317. doi: 10.1080/07357907.2018.1477955

Dawson, M., & Kouzarides, T. (2012). Cancer Epigenetics: From Mechanism to Therapy. Cell, 150(1), 12-27. doi: 10.1016/j.cell.2012.06.013

Li, E., & Zhang, Y. (2014). DNA Methylation in Mammals. Cold Spring Harbor Perspectives In Biology, 6(5), a019133-a019133. doi: 10.1101/cshperspect.a019133

Martens-Uzunova, E., Böttcher, R., Croce, C., Jenster, G., Visakorpi, T., & Calin, G. (2014). Long Noncoding RNA in Prostate, Bladder, and Kidney Cancer. European Urology, 65(6), 1140-1151. doi: 10.1016/j.eururo.2013.12.003

Jalali, S., Kapoor, S., Sivadas, A., Bhartiya, D., & Scaria, V. (2015). Computational approaches towards understanding human long non-coding RNA biology. Bioinformatics, 31(14), 2241-2251. doi: 10.1093/bioinformatics/btv148

Hajjari, M., & Salavaty, A. (2015). HOTAIR: an oncogenic long non-coding RNA in different cancers. Cancer Biology & Medicine. 12(1), 1-9. doi: 10.7497/j.issn.2095-3941.2015.0006.

Li, J., Wang, J., Zhong, Y., Guo, R., Chu, D., Qiu, H., & Yuan, Z. (2017). HOTAIR: a key regulator in gynecologic cancers. Cancer Cell International, 17(65). doi: 10.1186/s12935-017-0434-6

Cai, B., Song, X., Cai, J., & Zhang, S. (2014). HOTAIR: a cancer-related long non-coding RNA. Neoplasma, 61(4), 379-391. doi: 10.4149/neo_2014_075

Yu, X., & Li, Z. (2015). Long non-coding RNA HOTAIR: A novel oncogene (Review). Molecular Medicine Reports, 12(4), 5611-5618. doi: 10.3892/mmr.2015.4161

Martínez-Fernández, M., Feber, A., Dueñas, M., Segovia, C., Rubio, C., & Fernandez, M. et al. (2015). Analysis of the Polycomb-related lncRNAs HOTAIR and ANRIL in bladder cancer. Clinical Epigenetics, 7(109). doi: 10.1186/s13148-015-0141-x

Yu, D., Zhang, C., & Gui, J. (2017). RNA-binding protein HuR promotes bladder cancer progression by competitively binding to the long noncoding HOTAIR with miR-1. Oncotargets And Therapy, 10, 2609-2619. doi: 10.2147/ott.s132728

Sun, X., Du, P., Yuan, W., Du, Z., Yu, M., Yu, X., & Hu, T. (2015). Long non-coding RNA HOTAIR regulates cyclin J via inhibition of microRNA-205 expression in bladder cancer. Cell Death & Disease, 6(10), e1907. doi: 10.1038/cddis.2015.269

A global reference for human genetic variation. (2015). Nature, 526, 68-74. doi: 10.1038/nature15393

Gong, W., Yin, J., Li, X., Fang, C., Xiao, D., & Zhang, W. et al. (2016). Association of well-characterized lung cancer lncRNA polymorphisms with lung cancer susceptibility and platinum-based chemotherapy response. Tumor Biology, 37(6), 8349-8358. doi: 10.1007/s13277-015-4497-5

Taheri, M., Habibi, M., Noroozi, R., Rakhshan, A., Sarrafzadeh, S., & Sayad, A. et al. (2017). HOTAIR genetic variants are associated with prostate cancer and benign prostate hyperplasia in an Iranian population. Gene, 613, 20-24. doi: 10.1016/j.gene.2017.02.031

Korpal, M., Puyang, X., Jeremy Wu, Z., Seiler, R., Furman, C., & Oo, H. et al. (2017). Evasion of immunosurveillance by genomic alterations of PPARγ/RXRα in bladder cancer. Nature Communications, 8(103). doi: 10.1038/s41467-017-00147-w

Okegawa, T., Ushio, K., Imai, M., Morimoto, M., & Hara, T. (2013). Orphan nuclear receptor HNF4G promotes bladder cancer growth and invasion through the regulation of the hyaluronan synthase 2 gene. Oncogenesis, 2(7), e58. doi: 10.1038/oncsis.2013.25

Min, L., Mu, X., Tong, A., Qian, Y., Ling, C., Yi, T., & Zhao, X. (2018). The association between HOTAIR polymorphisms and cancer susceptibility: an updated systemic review and meta-analysis. Oncotargets And Therapy, 11, 791-800. doi: 10.2147/ott.s151454

Li, J., Cui, Z., Li, H., Lv, X., Gao, M., & Yang, Z. et al. (2018). Long non-coding RNA HOTAIR polymorphism and susceptibility to cancer: an updated meta-analysis. Environmental Health And Preventive Medicine, 23(8). doi: 10.1186/s12199-018-0697-0

Hassanzarei, S., Hashemi, M., Sattarifard, H., Hashemi, S., Bahari, G., & Ghavami, S. (2017). Genetic polymorphisms of HOTAIR gene are associated with the risk of breast cancer in a sample of southeast Iranian population. Tumor Biology, 39(10), 1-8. doi: 10.1177/1010428317727539

Yan, R., Cao, J., Song, C., Chen, Y., Wu, Z., Wang, K., & Dai, L. (2015). Polymorphisms in lncRNA HOTAIR and susceptibility to breast cancer in a Chinese population. Cancer Epidemiology, 39(6), 978-985. doi: 10.1016/j.canep.2015.10.025

Weng, S., Wu, W., Hsiao, Y., Yang, S., Hsu, C., & Wang, P. (2018). Significant association of long non-coding RNAs HOTAIR genetic polymorphisms with cancer recurrence and patient survival in patients with uterine cervical cancer. International Journal Of Medical Sciences, 15(12), 1312-1319. doi: 10.7150/ijms.27505

Guo, L., Lu, X., Zheng, L., Liu, X., & Hu, M. (2016). Association of Long Non-Coding RNA HOTAIR Polymorphisms with Cervical Cancer Risk in a Chinese Population. PLoS One, 11(7), e0160039. doi: 10.1371/journal.pone.0160039

Li, H., Tang, X., Liu, Y., Li, W., Chen, Q., & Pan, Y. (2017). Association of Functional Genetic Variants of HOTAIR with Hepatocellular Carcinoma (HCC) Susceptibility in a Chinese Population. Cellular Physiology And Biochemistry, 44(2), 447-454. doi: 10.1159/000485011

Su, S., Hsieh, M., Lin, C., Chuang, C., Liu, Y., Yeh, C., & Yang, S. (2018). Impact of HOTAIR Gene Polymorphism and Environmental Risk on Oral Cancer. Journal Of Dental Research, 97(6), 717-724. doi: 10.1177/0022034517749451

Wang, C., Li, Y., Li, Y., Zhang, H., Gong, H., & Yuan, Y. et al. (2018). HOTAIR lncRNA SNPs rs920778 and rs1899663 are associated with smoking, male gender, and squamous cell carcinoma in a Chinese lung cancer population. Acta Pharmacologica Sinica, 39(11), 1797-1803. doi: 10.1038/s41401-018-0083-x

Yang, X., He, J., Chang, Y., Luo, A., Luo, A., & Zhang, J. et al. (2018). HOTAIRgene polymorphisms contribute to increased neuroblastoma susceptibility in Chinese children. Cancer, 124(12), 2599-2606. doi: 10.1002/cncr.31353

How to Cite

1.
Volkohon AD, Chumachenko YD, Harbuzova VY, Ataman OV. Association analysis between rs1899663 HOTAIR gene polymorphism and bladder cancer development in Ukrainian population. Zaporozhye Medical Journal [Internet]. 2019Dec.11 [cited 2024Dec.23];(6). Available from: http://zmj.zsmu.edu.ua/article/view/186498

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Original research