UV spectrophotometry of the Metronidazole
DOI:
https://doi.org/10.14739/2310-1210.2013.5.19031Keywords:
Metronidazole, UV-spectrum, solutes, quantitative determinationAbstract
Objective. The purpose of this research was to study the UV spectrum of Metronidazole in different polarity solvents (water, 95 % ethanol, 0,1 M sodium hydroxide, 0,1 M hydrochloric acid and 5 M sulfuric acid solution), to establish the relationship between chemical structure of the analyzed compounds and the nature of their electronic spectrum. In addition, a detailed study of the ultraviolet spectra of Metronidazole in different solutions enables to select the optimal wavelength and a thinner design for Metronidazole in substance and dosage forms.
Materials and methods. Spectrophotometer SPECORD 200-222U214 has been used for study of the UV spectra of Metronidazole, the quantitative methods were used.
Measuring of the absorption of Metronidazole solutions has been carried out in quartz cuvettes with a layer thickness of 10 mm. Due to the fact that the test material shows selective absorption in the ultraviolet spectra, model compounds were studied at a concentration of 1 mg%. Study of electronic spectra was performed in the range of 200 to 400 nm, and the curve has been constructed in coordinates A = f (λ). It has been used water, 95% ethanol, 0,1 M NaOH, 0,1 M HCl and 5 M H2SO4 as solvents.
The content of Metronidazole in substance and dosage forms calculated with the equations according to SPU.
The results. Study of the UV spectra of Metronidazole and its model compounds showed that in short-wave part of spectrum due to absorption and excitation electrons of imidazole ring were transferred, and in long-wave – conjugation chromophore imidazole cycle and nitro group as electron acceptor occurred. Analysis of the UV spectra of Metronidazole enables to determine a maximum of the analytical quantification of the medicine.
Conclusions.
Metronidazole UV spectra in water, 95% ethanol, 0,1 M solution of NaOH, 0,1 M solution of HCl and 5 M solution of H2SO4 have been studied.
Metronidazole UV spectra are characterized by two absorption bands in the range 228-230 nm and 276-332 nm in specifying solvents.
Quantitative determination methods of Metronidazole in the substance (water and 95% ethanol at 230 nm), 0,5% solution for infusion (water at 230 nm) and vaginal suppositories of 0,1 g (water at 230 nm) have been developed and proposed. The error in the determination of metronidazole in the substance did not exceed ± 0,03, and in dosage forms - ± 0,05.
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