The level of serum and vaginal immunoglobulins in experimental vaginitis and its pharmacological correction


  • O. V. Dolzhykova National University of Pharmacy, Kharkiv, Ukraine,
  • R. F. Yeromenko National University of Pharmacy, Kharkiv, Ukraine,
  • V. V. Kozar National University of Pharmacy, Kharkiv, Ukraine,
  • O. M. Lytvynova National University of Pharmacy, Kharkiv, Ukraine,



vaginitis, immunoglobulins A, M, G, secretory Ig A, vaginal washings, suppositories Melanizol and Klimedeks



The problem of searching effective drugs for the treatment of infectious and inflammatory diseases of the female genital tract, which are accompanied by changes in the immune system, remains relevant.

The purpose of the study to investigate the level of immunoglobulins A, M, G and secretory immunoglobulin A in blood serum and vaginal washings in experimental vaginitis and in conditions of using new vaginal suppositories Melanizol and Klimedeks.

Materials and methods. The study was conducted using non-linear sexually mature female rats weighing 190 ±10 g. Vaginitis was modeled by a single intravaginal application of an irritant, presented by the mixture of terebenthine oil with dimethyl sulfoxide. We studied the effect of the vaginal suppositories Melanizol and Klimedeks and the reference drugs: suppositories Gravagin, vaginal tablets Micogynax and Suppositories with Hippophaes oleum. At the end of the 8-day experimental period, the concentration of immunoglobulins IgA, IgM, IgG and secretory immunoglobulin A (sIgA) in blood serum and vaginal washings were studied.

Results. The absence of significant changes in the concentration of Ig A, M, G in the serum of rats with experimental vaginitis and the tendency to increase in levels of Ig A, M, G in vaginal washings compared with indicators in intact animals. The serum and vaginal sIg A values were 2.5 and 1.4 times decreased in rats with vaginitis, respectively, compared with the intact controls. All studied drugs increased the concentration of the secretory immunoglobulin, and suppositories Klimedeks had a more significant effect.

Conclusions. Experimental vaginitis is characterized by a tendency to an increase in the concentration of Ig A, M, G in the vaginal washings and a significant decrease in the levels of serum and vaginal sIg A. The studied suppositories Melanizole and Klimedeks are as effective as Gravagin, Micogynax and Suppositories with Hippophaes oleum.


Turula, H., & Wobus, C. (2018). The Role of the Polymeric Immunoglobulin Receptor and Secretory Immunoglobulins during Mucosal Infection and Immunity. Viruses, 10(5), Article 237.

Kumar, B. V., Ma, W., Miron, M., Granot, T., Guyer, R. S., Carpenter, D. J., Senda, T., Sun, X., Ho, S.-H., Lerner, H., Friedman, A. L., Shen, Y., & Farber, D. L. (2017). Human Tissue-Resident Memory T Cells Are Defined by Core Transcriptional and Functional Signatures in Lymphoid and Mucosal Sites. Cell Reports, 20(12), 2921–2934.

Wang, Y., Sui, Y., Kato, S., Hogg, A. E., Steel, J. C., Morris, J. C., & Berzofsky, J. A. (2015). Vaginal type-II mucosa is an inductive site for primary CD8+ T-cell mucosal immunity. Nature Communications, 6, Article 6100.

Wira, C. R., Rodriguez-Garcia, M., & Patel, M. V. (2015). The role of sex hormones in immune protection of the female reproductive tract. Nature Reviews Immunology, 15, 217–230.

Lorenzen, E., Follmann, F., Jungersen, G., & Agerholm, J. S. (2015). A review of the human vs. porcine female genital tract and associated immune system in the perspective of using minipigs as a model of human genital Chlamydia infection. Veterinary Research, 46, Article 116.

Amjadi, F., Salehi, E., Mehdizadeh, M., & Aflatoonian, R. (2014). Role of the innate immunity in female reproductive tract. Advanced biomedical research, 3, 1.

Oh, J. E., Iijima, N., Song, E., Lu, P., Klein, J., Jiang, R., Kleinstein, S. H., & Iwasaki, A. (2019). Migrant memory B cells secrete luminal antibody in the vagina. Nature, 571(7763), 122–126.

Kalia, N., Singh, J., & Kaur, M. (2019). Immunopathology of Recurrent Vulvovaginal Infections: New Aspects and Research Directions. Frontiers in Immunology, 10, Article 2034.

Herbst-Kralovetz, M. M., Pyles, R. B., Ratner, A. J., Sycuro, L. K., & Mitchell, C. (2016). New Systems for Studying Intercellular Interactions in Bacterial Vaginosis. The Journal of Infectious Diseases, 214(Suppl. 1), S6–S13.

Bradshaw, C. S., & Brotman, R. M. (2015). Making inroads into improving treatment of bacterial vaginosis – striving for long-term cure. BMC Infectious Diseases, 15, 292.

Thinkhamrop, J., Hofmeyr, G. J., Adetoro, O., Lumbiganon, P., & Ota, E. (2015). Antibiotic prophylaxis during the second and third trimester to reduce adverse pregnancy outcomes and morbidity. Cochrane Database Of Systematic Reviews, (1), Article CD002250.

European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes, March 18, 1986,

Rudko, A. I., Solntseva, A. V., Usoeva, L. A., & Moruhina, L. V. (2011). Farmatsevtychna kompozytsiia dlia likuvannia zakhvoriuvan urohenitalnykh orhaniv [Pharmaceutical composition for the treatment of urogenital organs] (Ukraina. Patent No. 95741). Ukraina. Derzhavna sluzhba intelektualnoi vlasnosti Ukrainy. [in Ukrainian].

Rybolovlev, Yu. R., & Rybolovlev, R. S. (1979). Dozirovanie veshchestv dlya mlekopitayushchikh po konstantam biologicheskoi aktivnosti [The substance dosing for mammals according to biological activity constants]. Doklady Akademii Nauk SSSR, 247(6), 1513–1516. [in Russian].

Vlachou, M., Geraniou, E., & Siamidi, A. (2020). Modified release of furosemide from Eudragits® and poly(ethylene oxide)-based matrices and dry-coated tablets. Acta Pharmaceutica, 70(1), 49–61.

Chen, A., McKinley, S. A., Shi, F., Wang, S., Mucha, P. J., Harit, D., Forest, M. G., & Lai, S. K. (2015). Modeling of Virion Collisions in Cervicovaginal Mucus Reveals Limits on Agglutination as the Protective Mechanism of Secretory Immunoglobulin A. PLOS ONE, 10(7), Article e0131351.

How to Cite

Dolzhykova OV, Yeromenko RF, Kozar VV, Lytvynova OM. The level of serum and vaginal immunoglobulins in experimental vaginitis and its pharmacological correction. Zaporozhye Medical Journal [Internet]. 2020Apr.13 [cited 2024Jul.17];22(2). Available from:



Original research