Formation of biofilms by representatives of the oral microflora on the surfaces of basic materials
DOI:
https://doi.org/10.14739/2310-1210.2021.4.229814Keywords:
oral microflora, dental materials, biofilms, denture partial removable, integral coefficientsAbstract
The aim of the work is to conduct a comparative analysis of the biofilm formation by representatives of the oral microflora on the surfaces of basic materials.
Materials and methods. The process of biofilm formation was examined on 7 types of basic plastic samples: Polyan, Breflex, Nylon, Protakryl, Vinakryl, Biocryl, which were used for the manufacture of removable prosthetic basis constructions, and SYNMA, which was used for comparison. Biofilm formation was analyzed by the method Y. Zhang (2017) with minor modifications. The test sample was placed in a test tube with 2.0 ml of nutrient broth Brain Heart Infusion to model the biofilm growth of microorganisms (HiMedia Laboratories Pvt. Ltd., India) supplemented with 1 % glucose, pre-inoculated with test strains at a final concentration of 1 × 104 CFU/ml. The strains were cultivated for 24 hours at a temperature of 37 °C under continuous stirring in a shaker MR-1 (SIA BIOS AN, Latvia) at 20 rpm. Evaluation of the biofilm massiveness was performed after gentian violet staining followed by elution of the stain with ethanol and registration of the eluent optical density (OD). The OD was measured with a Synergy™ HTX S1LFTA microplate multimode photometer (BioTek Instruments, Inc., USA) at 595 nm wavelength using Gen5™ Data Analysis Software. The number of viable bacterial cells in the formed biofilms was determined by the method of ten-fold serial dilutions. The obtained results were converted per unit area of the sample tested.
Processing of the results was performed using a two-sample t-test with the software package Statistica 13.0 and Microsoft Office Excel, the differences were considered statistically significant at a P value of < 0.05. Statistical analysis of the obtained data was presented as mean values of measurements ± standard deviation for three independent experiments.
Results. According to the microbiological analysis results it was found that α-hemolytic streptococci S. oralis and S. sanguinis showed the ability to form biofilms on the surfaces of basic materials, namely Protacryl and Vinacryl, the total biomass of S. sanguinis biofilms was 47.7 % (P < 0.01) and 14.7 % (P > 0.05) greater, respectively, in comparison to a glass slide. Inhibition of biofilm formation processes was observed on the surfaces of Nylon and Biocryl basic materials. S. oralis and S. gordonii showed the highest ability to survive in biofilms. The intensity of C. albicans biofilms formation on Biocryl basic materials, comparative plastics SINMA and Breflex basic materials was greater than on glass slides by 48.3 %, 43.0 % and 34.9 % (P < 0.01), respectively. The least massive C. albicans biofilms were formed on Breflex surfaces and SINMA comparative plastics in comparison to glass slides by 33.6 % and 24.8 % (P < 0.01), respectively. Both Candida strains had the highest level of fungal viability in biofilms on Breflex, Polyan and Protacryl basic materials (P < 0.01), and C. tropicalis biofilms on Biocryl and Vinacryl basic materials (P < 0.05). Integral coefficients indicated the inhibition of the oral microflora ability to form biofilms on the surfaces of basic materials.
Conclusions. Oral α-hemolytic and β-hemolytic streptococci have the ability to intensive biofilm growth on the surfaces of the basic materials Protacryl and Vinacryl. Oral Candida albicans form massive biofilms on the surfaces of Biocryl and Vinacryl basic materials and comparative SYNMA plastics. The basic materials Breflex, Nylon and comparative plastics SYNMA are the most inert to biofilm formation by the oral microflora representatives.
References
Rozhko, S., & Paliichuk, I. (2019). Study of Complications in Patients Using RemovableDentures Over Different Periods. Archive of Clinical Medicine, 25(2), Article E201923. https://doi.org/10.21802/acm.2019.2.3
Dmytryshyn, T. M. (2019). Diagnosis of oral hygiene status in people of different age groups and with different duration of removable dentures use with the help of a new computer program. Zaporozhye medical journal, 21(3), 382-385. https://doi.org/10.14739/2310-1210.2019.3.169196
Dmytryshyn, T. M. (2018). Analiz vzaiemozv’iazkiv mizh mikrobiolohichnymy ta biokhimichnymy, biofizychnymy pokaznykamy u patsiientiv, yaki korystuiutsia znimnymy protezamy. [Analysis of correlations between microbiological and biochemical, biophysical parameters in patients who use removable dentures]. Svit medytsyny ta biolohii, (2), 44-48. https://doi.org/10.26724/2079-8334-2018-2-64-44-48 [in Ukrainian].
Redushko, Yu., Dmytryshyn, T., & Rozhko, О. (2020). Klinichnyi stan tkanyn proteznoho lozha v patsiientiv, yaki korystuiutsia riznymy adhezyvnymy zasobamy dlia pokrashchennia fiksatsii znimnykh proteziv [Clinical condition of prosthetic bed tissues in patients who use different adhesive means to improve fixation of removable dentures]. Suchasna stomatolohiia, (1), 96-99. https://doi.org/10.33295/1992-576X-2020-1-96 [in Ukrainian].
Verbovska, R. I. (2019). Zastosuvannia likuvalno-profilaktychnoho kompleksu pry ortopedychnomu likuvanni patsiientiv, yaki zastosovuiut adhezyvni serednyky dlia pokrashchennia mikrobiolohichnoho statusu rotovoi porozhnyny [The use of therapeutic and prophylactic complex in orthopedic patients using adhesive means to improve the microbiological status of the oral cavity]. Svit medytsyny ta biolohii, (3), 23-28. https://doi.org/10.26724/2079-8334-2019-3-69-23-28 [in Ukrainian].
Redanz, S., Cheng, X., Giacaman, R. A., Pfeifer, C. S., Merritt, J., & Kreth, J. (2018). Live and let die: Hydrogen peroxide production by the commensal flora and its role in maintaining a symbiotic microbiome. Molecular Oral Microbiology, 33(5), 337-352. https://doi.org/10.1111/omi.12231
Abranches, J., Zeng, L., Kajfasz, J. K., Palmer, S. R., Chakraborty, B., Wen, Z. T., Richards, V. P., Brady, L. J., & Lemos, J. A. (2018). Biology of Oral Streptococci. Microbiology Spectrum, 6(5), Article 10.1128/microbiolspec.GPP3-0042-2018. https://doi.org/10.1128/microbiolspec.GPP3-0042-2018
Okahashi, N., Nakata, M., Sumitomo, T., Terao, Y., & Kawabata, S. (2013). Hydrogen Peroxide Produced by Oral Streptococci Induces Macrophage Cell Death. PLOS ONE, 8(5), Article e62563. https://doi.org/10.1371/journal.pone.0062563
Okahashi, N., Nakata, M., Kuwata, H., & Kawabata, S. (2016). Streptococcus oralis Induces Lysosomal Impairment of Macrophages via Bacterial Hydrogen Peroxide. Infection and Immunity, 84(7), 2042-2050. https://doi.org/10.1128/IAI.00134-16
Okahashi, N., Sumitomo, T., Nakata, M., Sakurai, A., Kuwata, H., & Kawabata, S. (2014). Hydrogen Peroxide Contributes to the Epithelial Cell Death Induced by the Oral Mitis Group of Streptococci. PLOS ONE, 9(1), Article e88136. https://doi.org/10.1371/journal.pone.0088136
Zhang, Y., Wang, Y., Zhu, X., Cao, P., Wei, S., & Lu, Y. (2017). Antibacterial and antibiofilm activities of eugenol from essential oil of Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf against periodontal pathogen Porphyromonas gingivalis. Microbial Pathogenesis, 113, 396-402. https://doi.org/10.1016/j.micpath.2017.10.054
Rozhko, S., & Kutsyk, R. (2019). Study of Early Adhesion of Some Oral Microflora Representatives to Basic Materials of Removable Dentures. Galician Medical Journal, 26(3), Article E201939. https://doi.org/10.21802/gmj.2019.3.9
Rozhko, S., & Kutsyk, R. (2019). The influence of base resin of removable dentures on the planktonic growth of individual representatives of oral microflora. Postępy Nauk Medycznych, (4), 131-135. https://doi.org/10.25121/PNM.2019.32.4.131
Maldonado, K., Xu, D., Wang, Y., Zhang, J. F., Hamdan, S., Wen, Z. T., Fidel, P. L., Noverr, M. C., & Xu, X. (2018). Mechanical and Physical Properties of Experimental Antifungal Denture Base Resins. Oral Health and Dental Studies, 1(1), Article 5. https://doi.org/10.31532/oralhealthdentstud.1.1.005
Budală, D. G., Bosînceanu, D. N., Surlari, Z., Virvescu, D. I., Baciu, R., Bida, F. C., Balcoș, C., & Țănculescu, O. (2020). Denture base resins and microbial adhesion-current trends. Romanian Journal of Medical and Dental Education, 9(5), 15-20. http://journal.adre.ro/denture-base-resins-and-microbial-adhesion-current-trends/
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